A method for producing endoglucanase by fermenting roughage with mixed culture of natural symbiosis

Abstract

The invention relates to the field of biotechnology renewable energy, in particular to a method for producing endoglucanase by fermenting roughage substrates with a natural symbiotic mixed culture YakQH5, wherein the mixed culture YakQH5 is composed of anaerobic fungi (Neocallimastix frontalis) Composed of Methanobrevibacter gottschalkii, it can degrade different roughages and produce endoglucanase with high enzymatic activity, among which the activity of endoglucanase produced by degrading wheat hulls can reach 1170mU, which has achieved remarkable results Adding compound antibiotics in the fermentation process can also prevent the mixed culture YakQH5 from being polluted by bacteria during the fermentation process, and further improve the efficiency of anaerobic fermentation. The mixed culture adopted in the present invention can be preserved in vitro for survival and passage, which is convenient for popularization and provides convenience for production.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a method for producing endoglucanase by fermenting roughage with natural symbiotic mixed culture. Background technique [0002] In recent years, with the increase of the population and the improvement of the quality of life in the world, the demand for energy has risen sharply. Traditional energy materials are facing the danger of depletion, and the use of these traditional energy materials produces a large amount of greenhouse gases and pollutes the environment. Alleviating the three major crises of "food, energy and environment" facing mankind today is one of the important ways to realize the sustainable development strategy of agriculture. Rough fodder such as straw, hay and husks has received more and more attention as renewable energy materials. Roughage refers to feed with a natural moisture content below 60%, crude fiber content in dry matter equal to or hig...

AI Technical Summary

Problems solved by technology

The inventor first studied the anaerobic fermentation of the co-culture of grazing yak rumen anaerobic fungi and methanobacteria using wheat straw, corn straw and rice straw as substrates (Wei Yaqin. Research on the diversity of culture and its fiber degradation characteristics [D]. 2016.), by detecting gas production, polysaccharide hydrolase activity, various esterase activities, dry matter degradation rate, phenolic acid release, methane and acetic acid production Evaluate the effectiveness of the co-culture of anaerobic fungi and methanogens in degrading straw, among which the co-cultures of anaerobic fungi and methanogens that efficiently degrade the above three straws are N.frontalis Yak16+M.ruminantium, Piromyces Yak18+M.ruminantium and O.joyonii The endoglucanase activity produced by Yak7+M.ruminantium degrading the three major straws respectively is lower than 200mU, which cannot achieve the effect of producing endoglucanase with high enzyme activity

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