45 results about "APOPTOGENIC PROTEIN" patented technology

Lactobacillus plantarum DP189 and its application relate to the field of functional food microorganisms. The strain was deposited in the China Center for Type Culture Collection on March 25, 2019, and the preservation number is: CCTCC NO: M2019199. The strain can improve the learning and memory ability of depressed rats; increase the level of brain-derived neurotrophic factor in depressed rats; reduce the damage of hippocampal neurons and apoptosis of neurons in depressed rats; reduce the apoptosis of brain tissue in depressed rats The content of protein JUK2 and Bcl-2; reduce the abundance of Verrucomicrobiaceae in the intestine, increase the abundance of Lactobacillus in the intestine, increase the diversity of intestinal flora α-diversity and improve the intestinal flora disorder of depressed rats; thus It has good preventive and therapeutic effects on depression / anxiety, autism, and inflammatory bowel disease. The strain has antidepressant function, and can be used as a functional probiotic for the prevention and treatment of depression, anti-autism, and anti-inflammatory enteritis, and provides new ideas for clinical intervention and control of depression and other related mental diseases.

Disclosed are a compound of formula (I) that inhibits the activity of an anti-apoptotic Bcl-xL protein, a composition containing the compound and a method for treating a disease that expresses a Bcl-xL protein during a disease, such as cancer; X is a heteroaryl group; Y1 is phenylene or heteroarylene; L1 is a linking group; Y2 is cycloalkyl, cycloalkenyl, heterocycloalkyl or heterocycloalkenyl; Z1 is C(O)OR9, C(O)N10R11, C (O)R11, NR10C(O)R11, etc.

In the invention, polysaccharides are extracted from wolfberry black fruit, and the content of polysaccharides is determined by using anthrone-ethyl acetate-concentrated sulfuric acid solution. Crushing, soaking, heating, filtering, suction filtration and other treatments are performed on the fruit of Lycium barbarum to obtain a solution containing Lycium barbarum polysaccharide. The extracted Lycium barbarum polysaccharides were extracted with water, alcohol-precipitated, and suction-filtered, and the filter cake was dried in an oven. The polysaccharide powder was taken separately, dissolved after adding DMSO, stirred for 12 hours under a magnetic stirrer, added distilled water, and suction-filtered. The precipitate is washed successively with absolute ethanol, acetone and ether, and dried to obtain the refined Lycium ruthenicum polysaccharide; after the obtained refined Lycium ruthenicum polysaccharide is decolorized by macroporous resin and deproteinized by Sevage method, the black fruit Lycium barbarum polysaccharide. Refining with DMSO greatly increases the yield of polysaccharides. When the Lycium barbarum polysaccharides are applied to K562 cells, they can promote cell apoptosis by changing the expression of apoptotic proteins in the mitochondrial pathway, and have anti-leukemic tumor effects.

The invention relates to an application of a recombinant ganoderma lucidum immunoregulatory protein (rLZ-8) in preparation of medicines for treating chronic cardiac failure. According to the invention, a rat cardiac failure model is established by intraperitoneal injection of adriamycin amycin (ADR) to study the curative effect of rLZ-8 to chronic cardiac failure. Experimental research results show that rLZ-8 can remarkably improve the cardiac function of the rat cardiac failure model, reduce the rate of myocardial apoptosis, reduce expression of relevant apoptin in myocardial cells (caspase3 and caspase9) and increase the survival rate of rats with chronic heart failure.

The invention discloses an apoptosis protein fusion type anti-HER-2 single chain antibody and its preparation method and application. The apoptotic protein is cytochrome C or DNA fragmentation factor 40, which can be conjugated with anti-HER-2 single-chain antibody to form DFF40-scFv fusion single-chain antibody, nCytc (tandem)-scFv fusion single-chain antibody. The apoptotic protein fusion anti-HER-2 single chain antibody is inserted into the upstream of the scFv by the cDNA sequence of the apoptotic protein and cloned into the expression vector to construct a recombinant plasmid, and the recombinant plasmid is transferred into cells to induce the expression of the apoptotic protein fusion anti-HER-2 single chain antibody, the preparation method is easy to construct and express, and the constructed apoptotic protein fusion anti-HER-2 single-chain antibody can specifically target malignant tumors with high expression of HER-2 and mediate apoptosis of cancer cells, and can be used to prepare targeted Drugs to treat cancers with high expression of HER‑2.

The invention discloses a virus-like particle containing CASP8 apoptosis protein, a preparation method and application thereof, and belongs to the technical field of genetic engineering proteins. The virus-like particle containing the CASP8 apoptosis protein is obtained by co-transfecting the pSVCMV-Gag-CASP8 plasmid, the packaging plasmid pCMV-Δ8.2 and the VSV-G plasmid into cells, packaging, releasing, purifying and concentrating; the pSVCMV-Gag- The CASP8 plasmid is constructed by fusing the full-length CASP8 protein to the C-terminus of HIV‑1 Gag (Pr55). The preparation method of the present invention has simple preparation process, easy purification and uniform structure, and the virus-like particle containing CASP8 apoptosis protein prepared by the method has a strong therapeutic effect of inducing tumor cell death and effectively inhibiting tumor cell growth.

The invention discloses a preparation method of a compound PGHG in a medicine for treating parkinson's disease. The method comprises the following steps: performing ultrasonic extraction on Penthorumchinense Pursh. stem dry powder by using 70 percent of ethanol (1: 25), sequentially performing extraction by using petroleum ether and ethyl acetate to obtain extracting solutions; performing decompressing concentration and lyophilization on the extracting solutions to obtain Penthorum chinense Pursh. polyphenol fragments (PSE); separating the PSE through high-speed countercurrent chromatographyto obtain flow fractions A and B; further purifying the flow fraction A by adopting a preparative HPLC (High Performance Liquid Chromatography); manually collecting effluent liquid according to a chromatographic peak; performing decompressing concentration to obtain a monomer compound of which the purity is up to 99 percent; and the monomer compound is identified as PGHG through mass spectrometryand nuclear magnetic resonance spectroscopy. Due to the PSE and the PGHG, reduction of ATP level of 6-hydroxydopamine induced SH-SY5Y cells can be obviously suppressed, and the cell activity is improved. The PGHG can modulate and control expression of apoptosis protein and inflammatory protein through activating Nrf2 / HO-1 antioxidant pathway, and neuroprotection has an obvious effect on preventionand treatment of the parkinson's disease.