A kind of extraction method and application of polysaccharides from Lycium barbarum
The invention relates to a technology of Lycium barbarum nigra and an extraction method, which is applied in the field of polysaccharide extraction, can solve the problems of lowering activation energy, severe extraction conditions, large losses, etc., and achieves the effects of promoting expression, simple method and improved yield.
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Embodiment 1
[0023] Embodiment 1 A kind of extraction method of Lycium barbarum polysaccharide
[0024] Include the following steps:
[0025] (1) After washing the black wolfberry, dry it at 40°C, dehydrate, pulverize, pass through a 300-mesh sieve, and dry to obtain the black wolfberry powder;
[0026] (2) Add Lycium barbarum powder into distilled water, stir evenly, extract at 70° C. for 3 hours, and filter to obtain the extraction solution of Lycium barbarum polysaccharide; The liquid ratio is 1:25g / mL;
[0027] (3) adding ethanol to the extraction solution of Lycium barbarum polysaccharide in step (2), after the alcohol meter detects that the alcohol content is 90%, carry out suction filtration, place the obtained filter cake in an oven and dry at 60° C. for 30 minutes, Obtain polysaccharide powder;
[0028] (4) After adding 50 mL of DMSO to 5 g of polysaccharide powder obtained in step (3), dissolve it, stir it magnetically for 12 hours, add 50 mL of distilled water, continue to st...
experiment example 1
[0030] The drawing of experimental example 1 glucose standard curve:
[0031] (1) Preparation of anthrone-ethyl acetate solution
[0032] Weigh 1g of anthrone into a 50mL volumetric flask, slowly add ethyl acetate to volume, shake well to dissolve, and store in a dark place for later use.
[0033] (2) Determine the maximum absorption wavelength:
[0034] Weigh 0.1g of glucose powder with an analytical balance and place in a 100mL volumetric flask to constant volume, prepare a glucose standard solution with a concentration of 1mg / mL, and pipette 0, 0.1, 0.2, 0.3, 0.4, 0.5mL of glucose standard solutions to 10mL, the final concentration of glucose is 0μg / mL, 10μg / mL, 20μg / mL, 30μg / mL, 40μg / mL, 50μg / mL respectively, take 2mL of the glucose solution of the above concentration and add 0.5mL of anthrone-ethyl acetate, 5mL concentrated sulfuric acid solution, shake well, scan at a wavelength of 500-800nm, and find that the absorption value at 620nm is the largest, so this wavelengt...
Embodiment 2
[0037] Embodiment 2 Different solid-liquid ratios influence polysaccharide extraction yield
[0038] Weigh 5 portions of 10g black wolfberry powder and add different volumes of aqueous solutions to make the solid-liquid ratio (g / mL) 1:10, 1:15, 1:20, 1:25, 1:30. Stir the glass rod to fully soak it, extract at 90°C for 2 hours, filter it with gauze, filter it with a Buchner funnel, take the filtrate, and add anthrone (C 14 h 10 O)-Ethyl acetate (CH 3 COOC 2 h 5 ) solution (0.5mL), concentrated sulfuric acid (H 2 SO 4 ) solution (5mL), put it into boiling water for 1 min, take it out immediately and cool it at room temperature, use distilled water as a blank control, measure the absorbance at 620nm with a UV-visible spectrometer and adjust the concentration so that the absorbance value is between 0.2-0.7, compare with the standard curve , to obtain the concentration of Lycium barbarum polysaccharide, and the results are shown in Table 1.
[0039] Table 1 Effect of solid-l...
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