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A virus-like particle comprising casp8 apoptosis protein and its preparation method and application

An apoptotic protein, virus-like technology, applied in the field of virus-like particles containing CASP8 apoptotic protein and its preparation, can solve problems such as development or metastasis, cell apoptosis, tumorigenesis, etc., achieve uniform structure, easy purification and preparation Ease of the process

Active Publication Date: 2021-07-27
遵义医科大学珠海校区
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Problems solved by technology

Some oncogenic mutations disrupt apoptosis, leading to tumor initiation, progression, or metastasis; on the other hand, silencing of several tumor suppressor genes may lead to an increased risk of tumor formation and progression

Method used

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  • A virus-like particle comprising casp8 apoptosis protein and its preparation method and application
  • A virus-like particle comprising casp8 apoptosis protein and its preparation method and application
  • A virus-like particle comprising casp8 apoptosis protein and its preparation method and application

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Embodiment Construction

[0050] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0051] A virus-like particles comprising Casp8 apoptosis protein, the virus-like particles consisting of PSVCMV-Gag-Casp8 plasmid, packaging plasmid PCMV-Δ8.2 and VSV-G (hydrobiobiobitis viral envelope G glycoprotein) plasmid Transfected into the cell post-release release, concentrated purification; the PSVCMV-Gag-Casp8 plasmid was constructed by the Full length Casp8 protein to the C-terminal of the HIV-1 PR55. The amino acid sequence of the virus-like particles is shown in Seq ID NO.1 in the sequence list. The base sequence of the virus-like particles is shown in SEQ ID NO.2 in the sequence list.

[0052] The preparation method of virus-like particles containing Casp8 apoptosis is included as described above includes the following steps:

[0053] Step 1: Plasmid construction:

[0054] A VLPS similar to HIV-1 can be formed only by HIV-1 structural ...

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Abstract

The invention discloses a virus-like particle containing CASP8 apoptosis protein, a preparation method and application thereof, and belongs to the technical field of genetic engineering proteins. The virus-like particle containing the CASP8 apoptosis protein is obtained by co-transfecting the pSVCMV-Gag-CASP8 plasmid, the packaging plasmid pCMV-Δ8.2 and the VSV-G plasmid into cells, packaging, releasing, purifying and concentrating; the pSVCMV-Gag- The CASP8 plasmid is constructed by fusing the full-length CASP8 protein to the C-terminus of HIV‑1 Gag (Pr55). The preparation method of the present invention has simple preparation process, easy purification and uniform structure, and the virus-like particle containing CASP8 apoptosis protein prepared by the method has a strong therapeutic effect of inducing tumor cell death and effectively inhibiting tumor cell growth.

Description

Technical field [0001] The present invention relates to the field of genetic engineering protein, and more particularly to virus-like particles comprising Casp8 apoptotic proteins and preparation methods and applications thereof. Background technique [0002] Breast cancer is the most common cancer worldwide, and is the fifth largest common cause of female cancer related death. In the past two decades, drug treatment of breast cancer has evolved into a more targeted method in addition to cytotoxic chemotherapeutic treatment, which greatly improves the prognosis of patients. However, metastatic breast cancer (MBC) is still unable to cure, estimating that the total survival rate of 5 years is only 23%. An important and unexpected discovery of breast cancer treatment is the emergence of drug-resistant cancer cells. Therefore, in order to further improve the clinical results and survival rates of breast cancer, new potential treatment strategies are still needed. [0003] Cell apopto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/00A61P35/00
CPCA61K39/0011A61K2039/5258A61P35/00C07K14/005C07K2319/00C12N9/6413C12N2740/16022C12N2760/20222
Inventor 敖竹君姚小剑陈伟殷立华薛亮刘振勇
Owner 遵义医科大学珠海校区
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