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Application of tartary buckwheat flavone in preparation of medicine for treating pancreatic cancer

A technology for tartary buckwheat flavonoids and pancreatic cancer, which is applied in the field of pharmaceutical industry, can solve problems such as large side effects and cannot be completely cured, and achieve the effects of low toxicity and side effects, reduction of diagnosis and treatment costs, and strong operability.

Pending Publication Date: 2022-01-04
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, chemotherapy and radiotherapy are mainly used for conservative treatment of pancreatic cancer, but they cannot be completely cured, and the side effects are large.

Method used

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  • Application of tartary buckwheat flavone in preparation of medicine for treating pancreatic cancer
  • Application of tartary buckwheat flavone in preparation of medicine for treating pancreatic cancer
  • Application of tartary buckwheat flavone in preparation of medicine for treating pancreatic cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of Tartary Buckwheat Flavonoids

[0053] Extract tartary buckwheat flour in ethanol with a concentration of 63% for 2 hours at a temperature of 70°C, filter with a multi-purpose vacuum pump for circulating water, collect the supernatant, and concentrate using a rotary evaporator for 30 minutes at a temperature of 60°C to obtain the extract, and then use 28% ethanol to dissolve the extract;

[0054] At room temperature, wash the AB-8 macroporous adsorption resin with 98% ethanol and put it on the column, inject the extract solution dissolved in ethanol into the column, and let it stand for 2 hours. After the end, slowly elute with water, discard Remove the washing liquid, then elute with 60% ethanol, collect the eluent, recover the ethanol under reduced pressure, concentrate and dry the tartary buckwheat flavonoids, and the obtained powder is the extracted tartary buckwheat flavonoids, which is analyzed by HPLC to obtain The content of total flavones in the ...

Embodiment 2

[0056] cell culture

[0057] Take out the complete cell medium (DMEM medium, purchased from Gibco, USA) 30 minutes in advance and return to room temperature, inoculate 10% fetal bovine serum and 0.1% penicillin-streptomycin in the DMEM medium to obtain 10% fetal bovine serum and DMEM medium with 0.1% penicillin-streptomycin double antibody. Afterwards, PANC-1 cells (pancreatic carcinoma ductal cells, purchased from American Type Culture Collection (ATCC, Manassas, VA, U.S.) in DMEM medium at 37°C, 5% CO 2 Cultured in a cell culture incubator. The medium was changed every 24 hours, and the PANC-1 cell culture operation was completed when the PANC-1 cells were in the logarithmic growth phase, and the cultured PANC-1 cells were collected for use.

Embodiment 3

[0059] Western Blot western blot detection

[0060] Get the PANC-1 cell that embodiment 2 cultivates and obtain, be divided into 3 groups on average, each group is inoculated in the 96-well plate after being digested with 0.25% trypsin, and each group has 6 duplicate holes, until the PANC-1 cell grows to 50~ When the density is 60%, perform Western Blot detection according to the following steps:

[0061] The first group: PANC-1 cells at the end of growth were not treated with any drugs, the total protein was extracted with RIPA cell protein lysate (purchased from Beyond Biotechnology Co., Ltd.), and the BCA detection kit (purchased from Beyond Biotechnology Co., Ltd.) company) to detect protein concentration. After the protein sample was quantified, it was mixed with Loading Buffer (5X) (purchased from Beyontian Biotechnology Co., Ltd.), boiled at 99°C for 10 minutes, and then used. SDS-PAGE electrophoresis, after the electrophoresis, put it into the transfer tank, store it...

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Abstract

The invention discloses application of tartary buckwheat flavone in preparation of a medicine for treating pancreatic cancer, and finds that the tartary buckwheat flavone can obviously promote apoptosis of pancreatic cancer cells, inhibit proliferation of the pancreatic cancer cells and break DNA (deoxyribonucleic acid) in the aspect of researching the activity effect of the tartary buckwheat flavone on the pancreatic cancer cells, and has huge potential in the aspect of exerting tumor resistance. The tartary buckwheat flavone is prepared from tartary buckwheat powder through alcohol extraction and resin purification, the preparation method is simple, when the tartary buckwheat flavone is used for preparing the medicine composition for preventing, treating or improving pancreatic tumors, the tartary buckwheat flavones can promote the expression of apoptosis protein Caspase-3, Caspase-8, Cleave-Caspase-3, Cleave-Casspse8, Cleave-caspase9 and Bax and inhibit expression of anti-apoptosis protein Bcl-2, it is proved that the tartary buckwheat flavone has the activity function of promoting apoptosis of the pancreatic cancer cells, and then the activity effect of resisting pancreatic cancer is achieved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical industry, and relates to the medicinal use of tartary buckwheat flavonoids, in particular to the use of tartary buckwheat flavonoids in preparing medicines for treating pancreatic cancer. Background technique [0002] Pancreatic cancer is a malignant tumor mainly derived from the pancreatic ductal epithelium. It is one of the common malignant tumors of the digestive system. Its incidence accounts for 1% to 2% of common malignant tumors. in women (1.7:1). In recent years, the incidence of pancreatic cancer has been on the rise worldwide. In the United States, there are about 27,000 new cases of pancreatic cancer each year, and 26,000 patients died of pancreatic cancer in the same year. Its case fatality rate ranks fifth in the United States. bit. The incidence of pancreatic cancer in my country is also increasing year by year. [0003] Pancreatic cancer is one of the malignant tumors with the worst...

Claims

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Application Information

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IPC IPC(8): A61K36/70A61P35/00A61P1/18
CPCA61K36/70A61P35/00A61P1/18
Inventor 廖志勇程文文蔡慈峰周美亮
Owner WENZHOU UNIVERSITY
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