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Lactobacillus plantarum dp189 and its application

A technology of Lactobacillus plantarum, DP189, applied in the directions of Lactobacillus, application, bacteria, etc., can solve problems such as unclear mechanism, and achieve the effect of broad application value, reduced content, good preventive and therapeutic effects

Active Publication Date: 2020-01-07
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that probiotics can reduce or prevent the development of intestinal diseases, such as diarrhea, inflammatory bowel disease, irritable bowel syndrome, etc., and 40-50% of patients with irritable bowel syndrome are accompanied by mental disorders such as anxiety and depression. Symptoms, some studies have shown that after the intervention of probiotics in patients with irritable bowel syndrome, the depression behavior is reduced while the intestinal discomfort is relieved, and the depression mood is improved, but the mechanism is still unclear

Method used

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  • Lactobacillus plantarum dp189 and its application
  • Lactobacillus plantarum dp189 and its application
  • Lactobacillus plantarum dp189 and its application

Examples

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preparation example Construction

[0078] The preparation method of fluoxetine hydrochloride solution: get the granule in the fluoxetine hydrochloride capsule, dissolve with 0.5g / 100ml sodium carboxymethylcellulose aqueous solution. The dose of fluoxetine hydrochloride is calculated as particulate matter.

[0079] The preparation method of the corticosterone solution: take the corticosterone powder, add 0.1% DMSO and 0.1% Tween, and dissolve it to a dose of 40 mg / kg with physiological saline.

Embodiment 1

[0080] Embodiment 1, isolation, identification and preservation of plantarum lactobacillus strain

[0081] 1. Isolation of Lactobacillus plantarum strains

[0082] In December 2017, fresh pickled sauerkraut samples were collected from Tonghua City, Jilin Province, put into transport medium, and quickly placed in an ice box and sent to the laboratory for strain isolation. After the sauerkraut sample was ground and serially diluted, the Lactobacillus in the sample was isolated using a Lactobacillus selective medium, cultured at 37°C for 48 hours, and typical colonies on the plate were picked (milky white, round, with neat edges, raised, Opaque, wet and smooth surface), streak culture MRS solid medium, and isolate pure colonies. The purely cultured strains were inoculated into liquid MRS medium for culture, then added with 60% glycerol, and stored in a -80°C refrigerator. One of the Lactobacillus strains was named DP189.

[0083] 2. Identification of strains

[0084] Physiolo...

Embodiment 2

[0090] Example 2. Protection of Lactobacillus plantarum DP189 on hippocampal neurotrophic pathway and inhibition of hippocampal neuron apoptosis

[0091] 1. Preparation of bacterial suspension

[0092] 1. Lactobacillus plantarum DP189 (hereinafter referred to as DP189) was inoculated in liquid MRS medium, cultured at 37°C for 16 hours, centrifuged at 4°C at 6000rpm for 8 minutes, and the bacterial precipitate was collected.

[0093] 2. Take the bacterial precipitate obtained in step 1 and suspend it with sterile physiological saline to obtain a bacterial concentration of 2×10 10 CFU / mL of DP189 bacterial suspension.

[0094] Two, group processing

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Abstract

Lactobacillus plantarum DP189 and its application relate to the field of functional food microorganisms. The strain was deposited in the China Center for Type Culture Collection on March 25, 2019, and the preservation number is: CCTCC NO: M2019199. The strain can improve the learning and memory ability of depressed rats; increase the level of brain-derived neurotrophic factor in depressed rats; reduce the damage of hippocampal neurons and apoptosis of neurons in depressed rats; reduce the apoptosis of brain tissue in depressed rats The content of protein JUK2 and Bcl-2; reduce the abundance of Verrucomicrobiaceae in the intestine, increase the abundance of Lactobacillus in the intestine, increase the diversity of intestinal flora α-diversity and improve the intestinal flora disorder of depressed rats; thus It has good preventive and therapeutic effects on depression / anxiety, autism, and inflammatory bowel disease. The strain has antidepressant function, and can be used as a functional probiotic for the prevention and treatment of depression, anti-autism, and anti-inflammatory enteritis, and provides new ideas for clinical intervention and control of depression and other related mental diseases.

Description

technical field [0001] The invention relates to the technical field of functional food microorganisms, in particular to a plant lactobacillus DP189 and its application. Background technique [0002] According to data released by the World Health Organization in 2017, the number of people affected by depression in the world has increased by 18% in the past 10 years. Depression has become an important disease that plagues human living standards and causes social and economic burdens around the world. With the rapid development of economy in our country, the rhythm of people's life and work has been significantly accelerated, and the accompanying psychological and mental pressure has generally increased, leading to an upward trend in the prevalence of mental disorders. According to statistics from the Ministry of Health, the prevalence of depressive disorder in my country is 3.59%, and more than 80% of depressive patients have not received standardized treatment, resulting in r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/24A61P1/00A23L33/135C12R1/25
CPCA61K35/747A61P25/24A23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2200/322
Inventor 李盛钰赵玉娟赵子健段翠翠王超高磊杨舸
Owner JILIN ACAD OF AGRI SCI
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