A method for detecting the content of
amitraz includes the following steps that firstly, an
amitraz standard substance is taken, standard solutions of a series of concentrations are prepared, detection is carried out with the
high performance liquid chromatography, high performance liquid chromatograms of the standard solutions of the series of concentrations are obtained, and an
amitraz standard curve is obtained; secondly, a sample to be detected is taken, a sample solution is prepared, detection is carried out with the
high performance liquid chromatography, and a high performance liquid chromatogram of the sample solution is obtained; thirdly, the content of amitraz in the sample to be detected is calculated with the external standard method according to the obtained high performance liquid chromatogram of the sample solution and the obtained amitraz
standard curve. According to the chromatographic conditions of the
high performance liquid chromatography adopted in the first step and the second step, octadecylsilane chemically bonded silica serves as a packed column, an
acetonitrile-
phosphoric acid solution serves as a mobile phase, the flow velocity of the mobile phase is 0.5-1.5 ml / min, the
column temperature is 20-40 DEG C, and the detection
wavelength is 250-350 nm. By the adoption of the high performance liquid
chromatography, the method for detecting the content of amitraz is easy, convenient and fast to implement, high in university, short in peak appearance time, capable of saving time and labor, economical and
environmentally friendly.