Preparation method and application of amitraz molecular imprinting monolithic column

Abstract

The invention discloses a preparation method and application of an amitraz molecular imprinting monolithic column. The preparation method comprises the following steps: by taking amitraz as a template molecule, dissolving the template molecule into a mixed solution of acetonitrile and dodecyl alcohol, adding a functional monomer, pre-polymerizing at a low temperature, further adding a cross-linking agent and an initiator, introducing nitrogen, filling into a chromatographic column hollow tube of which the lower end is already sealed, sealing the upper end of the chromatographic column hollow tube, and initializing polymerization, thereby obtaining the molecular imprinting monolithic column. After the functional monomer, a pore-foaming agent and the template molecule which are not polymerized are washed off from the column by using a mixed solution of methanol and acetic acid, the molecular imprinting monolithic column which has a good adsorption effect and an enrichment effect on amitraz is obtained. Compared with a conventional body polymerization method, the preparation method has the characteristics that the preparation method is simple and convenient, the aftertreatment is simple, the recognition property is good, the cost is low, required instruments and reaction conditions are easy to achieve, and the like. The molecular imprinting monolithic column prepared by using the preparation method is capable of separating and detecting residual amitraz in foods.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for preparing an amitraz molecularly imprinted monolithic column and its application in the analysis of amitraz residues in food. Background technique [0002] Amitraz is a broad-spectrum acaricide with moderate toxicity. It mainly inhibits the activity of monoamine oxidase. It has contact killing, antifeedant and repellent effects, and also has certain systemic and fumigation effects. Acaricide-resistant mites are also effective, and the number of harmful mites can be controlled for a long time after treatment. Amitraz is widely used in the prevention and control of harmful mites on fruit trees, vegetables, tea, cotton, soybeans, sugar beets and other crops. systemic and fumigation effects. It also has good medicinal effect on Homoptera pests, and can also be used to control pests such as aphids, cotton bollworms, and red flies. It also has a good control effect o...

AI Technical Summary

Problems solved by technology

Gas chromatography needs to derivatize amitraz, and the sample processing process is cumbersome; gas chromatography-mass spectrometry requires high equipment and is expensive, and the general laboratory cannot bear it; while liquid chromatography pretreatment is relatively simple, but It is necessary to purchase a specific chromatographic column, and the price is relatively high. Although the chromatographic column of biological antibodies can specifically recognize the target molecule, the preparation process of the antibody is complicated, the cost is high, the preparation cycle is long, and the tolerance to environmental conditions poor