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Method for detecting amitraz in food

A detection method, the technology of amitraz, which is applied in the field of gas phase-tandem mass spectrometry detection of amitraz in food, can solve the problems of high detection limit, small response value of SIM mode, unsatisfactory complex matrix, etc., so as to avoid false positives Effect

Inactive Publication Date: 2016-11-23
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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Problems solved by technology

The GC-MS method uses n-hexane to extract the target directly on the machine, and detects in the selected ion scanning (SIM) mode. The pretreatment in the method is simple, but the response value of the SIM mode is small, and the detection limit is still relatively high, which cannot meet the complex matrix ( Such as milk) and the detection of samples with high detection limit requirements

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  • Method for detecting amitraz in food
  • Method for detecting amitraz in food
  • Method for detecting amitraz in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 : Amethyst assay method.

[0036] 1. Reagents and solutions

[0037] Amitraz standard substance: content ≥99%;

[0038] 2,4-Dimethylaniline standard substance: content ≥95%;

[0039] 1mol / L sodium hydroxide solution: weigh 40g of sodium hydroxide, dissolve in water and dilute to 1000ml;

[0040] Alkaline aqueous solution: measure 1000ml of water, adjust the pH to 9.0 with 1mol / l sodium hydroxide solution;

[0041] Saturated sodium bicarbonate solution: Take 40g of sodium bicarbonate, dissolve it in water and dilute to 1000ml;

[0042]1mg / ml standard stock solution of amitraz and 2.4-dimethylaniline: Weigh 10mg each of the standard amitraz and 2,4-dimethylaniline, respectively, in a 10ml volumetric flask, dissolve with n-hexane and dilute to scale;

[0043] Sodium hydroxide: analytical grade, sodium bicarbonate: analytical grade, anhydrous sodium sulfate: analytical grade, heptachlorobutyric anhydride: chromatographic grade, n-hexane: chromatographic ...

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Abstract

The invention provides a method for detecting amitraz in food. The method includes the following steps: extracting to obtain extract; hydrolyzing the extract to obtain hydrolysate; deriving to obtain derivative liquid after hydrolysate and derivatizing agent are reacted; purifying derivative liquid to obtain a purification solution; and an instrument determination step of measuring the purification liquid by gas phase-tandem mass spectrometry. In this method, a parent ion and two daughter ions are selected for accurate characterization by GC-MS, which completely avoids the false positives caused by the matrix effect of the sample.

Description

technical field [0001] The invention relates to a method for detecting amitraz in food, in particular to a gas phase-tandem mass spectrometry method for detecting amitraz in food. Background technique [0002] Amitraz (N,N-bis(2,4-xylbeniminomethyl)methylamine) is an insecticide. In cotton, amitraz is used to control bollworms, whiteflies, and Spodoptera litura; in livestock, it is commonly used to control ticks, mites, lice, and other animal parasites. It is generally believed that amitraz is toxic, so many countries have established the maximum residue limit of amitraz in food. [0003] At present, the detection methods of amitraz include liquid chromatography, gas chromatography, gas chromatography-mass spectrometry and so on. The detection limit of liquid chromatography is high, the resolution is relatively poor, and the influence of interfering peaks is difficult to solve. Gas chromatography (GB 29707-2013 Determination of Amitraz Residue Marker Residues in Milk Gas ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 扎木则仁吴腾常建军魏文平其其格宋晓东赵桂荣
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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