Method of measuring residual quantity of amitraz and metabolites in honey by means of QuEChERS-liquid chromatogram-mass spectrum/mass spectrum method

Abstract

The invention relates to a method of measuring residual quantity of amitraz and three metabolites, in particular to a method of measuring residual quantity of amitraz and metabolites in honey by meansof a QuEChERS-liquid chromatogram-mass spectrum / mass spectrum method. The method comprises the following steps: adding a buffer solution, the pH of which is 7, into a test sample; carrying out extraction and salting out with acetonitrile and sodium chloride; carrying out dispersive solid-phase extraction on an adsorbent containing N-primary secondary amine (PSA), anhydrous magnesium sulfate and C18 powder to purify an extraction solution; carrying out detection by a liquid chromatogram-mass spectrum / mass spectrometer; and carrying out quantification by means of a isotope interior label diluted internal standard method. The method is green and environmentally friendly, simple and rapid, small in consumed resource and low in detection cost, and the sensitivity of the method can meet the detection and confirmation requirements on the residual quantity of amitraz and the three metabolites in honey.

Description

technical field [0001] The present invention relates to a method for determining the residues of amitraz and its three metabolites in honey, in particular to a QuEChERS-liquid chromatography-mass spectrometry / mass spectrometry method for the simultaneous determination of amitraz and monoformamidine 2,4-dimethazine in honey Method for residues of methylphenylformamide and 2,4-dimethylaniline. Background technique [0002] Honey itself is rich in more than 200 types of nutrients such as multivitamins, minerals and amino acids, as well as simple sugars (glucose, fructose) that can be directly absorbed by the human body, and it has good effects on constipation, gastric and duodenal ulcers, etc. It can assist medical treatment and improve human immunity, so it is more and more accepted by consumers as a nutritious food. With the improvement of people's living standards, the demand for honey is also increasing, and its quality and safety are directly related to human health. Amo...

AI Technical Summary

Problems solved by technology

2,4-Dimethylaniline is usually used as a raw material for the synthesis of monoformamidine and amitraz in chemical synthesis, but studies have shown that 2,4-Dimethylaniline has toxic side effects and mutagenicity, and is harmful to the liver. Larger damage

This patent is applicable to the detection of amitraz and its metabolite residues in royal jelly, and needs to be improved in terms of detection speed, green detection and cost saving

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