239 results about "Amino acid fermentation" patented technology

The invention belongs to the field of fermented waste liquid treatment in the biological fermentation industry and discloses a fertilizer prepared by utilizing threonine fermented wastes. The fertilizer is prepared by the following steps: (1) preparing threonine fermentation liquor; (2) filtering and discoloring; (3) separating crystals and a mother solution; (4) hydrolyzing mycoprotein; (5) preparing feed liquid A; and (6) preparing the fertilizer. According to the fertilizer, amino acid fermented wastes are effectively utilized, the production cost of the fertilizer is reduced, the wastes are changed into valuable materials, the fertilizer efficiency is lasting, and the fertilizer is superior to like products in the market.

A fermented organic amino acid fertilizer contains agricultural amino acid powder containing K (5-15%), fermented organic substance prepared from mud, animal's droppings, beer dregs, plant cake and peat (60-80%), and inrorganic fertilizer contains trace elements (15-40%). Its advantages are high utilization rate, high effect on improving soil and quality of crops and low cost.

The invention discloses an amino acid fermentation bacteria utilization method, which belongs to the technical field of amino acid production. The method comprises the steps that by taking glutamic acid fermentation as an example and adopting the isoelectric point of fermentation broth, a disc separator is utilized to separate mycoprotein in the fermentation broth, and a moderate amount of compound enzyme is added into the mycoprotein serum, so that the walls of bacteria are broken and the bacteria are enzymetically hydrolyzed. The enzymatic hydrolysate is separated by the high-speed disc separator, and the cell walls are removed, the obtained clarified enzymatic hydrolysate is concentrated under low temperature to produce the enzymetically hydrolyzed mycoprotein extract, or the clarified enzymatic hydrolysate is dried by a gunite granulation fluidized bed drier, a spray granulation drier and the like to produce the enzymetically hydrolyzed mycoprotein powder. In the method, the utilization value of the mycoprotein is greatly increased, so that the mycoprotein is changed from cheap feed into high value-added yeast extract and yeast powder substitute, the product can be used as fermentation ingredient and in other fields, the resources can be sufficiently utilized in the whole process, the purposes of energy saving and consumption reduction are achieved, and the invention has a broad application prospect.

The invention discloses a soil improving synergistic saline-alkali resistant water soluble fertilizer and a preparation method thereof. The waste liquid generated in the amino acid fermentation production process is taken as a carrier, and added with active humic acid prepared from decomposed coal or brown coal or peat rich in humic acid, and then the mixture is thoroughly and proportionally mixed with water soluble nitrogen fertilizer, water soluble phosphatic fertilizer, water soluble potassic fertilizer, and macroelements and microelements such as copper sulfate, borax, zinc sulfate and ferrous sulfate; and then the final mixture is subjected to spray drying, separation and collection treatment, so that a powdery material is obtained; and as a result, the soil improving synergistic saline-alkali resistant water soluble fertilizer is formed. The soil improving synergistic saline-alkali resistant water soluble fertilizer provided by the invention is low in cost, low in investment, low in energy consumption, small in floor area, energy-saving, environment-friendly and free of pollution; zero emission of amino acid waste liquid and deep processing of the decomposed coal, the brown coal and the peat are realized and pollution on the environment is reduced; the waste is well utilized and changed into things of value; besides, the soil improving synergistic saline-alkali resistant water soluble fertilizer is good in water solubility, free of residue, rich in nutrition, high in active organic matter content and high in safety; and furthermore, the soil improving synergistic saline-alkali resistant water soluble fertilizer achieves the effects or improving the soil and resisting saline and alkali, and is obvious in synergia and wide in uses.

The invention belongs to the field of treatment of fermentation waste liquid in biological fermentation industry and discloses a biological bacterial fertilizer prepared from glutamic acid fermentation wastes. The fertilizer is prepared by the following steps: S1) filtering the fermentation liquid; S2) decoloring, concentrating and crystallizing; S3) hydrolyzing; S4) preparing slurry B; and S5) preparing the biological bacterial fertilizer. The biological bacterial fertilizer disclosed by the invention effectively utilizes amino acid fermentation wastes, thereby lowering the production cost of the fertilizer and turning wastes into wealth. Moreover, the fertilizer which is durable is superior to similar products on the market.

The invention discloses a multifunctional compound fertilizer which is prepared from the raw materials including urea, ammonium sulfate, monoammonium phosphate, potassium chloride, silico-calcium fertilizer, ammonium lignosulphonate, amino acid, fermented rape seed cake, zinc sulfate, magnesium sulfate, borax, kieselguhr, magnesia, ammonium molybdate and polyaspartic acid through crushing, mixing and pelletizing. The multifunctional compound fertilizer contains not less than 35 percent of total nutrients of nitrogen, phosphorus and potassium, not less than 15 percent of organic matters, not less than 6 percent of amino acid, not less than 5 percent of humic acid, not less than 6 percent of trace elements and not less than 0.15 percent of synergist. The multifunctional compound fertilizer has functions of resisting insects and diseases, controlling loss, increasing effect and efficiency, maintaining land, and protecting environment.

The invention discloses a liquid bacterial agent for rooting, which comprises the following raw materials by weight parts: 20-80 parts of concentrated amino acid fermented liquid, 15-30 parts of urea, 5-20 parts of potassium fulvic acid, 10-20 parts of alginic acid, 5-15 parts of chitosan, 10-30 parts of bacillus subtilis, 5-20 parts of Alpha-pimacol, 10-35 parts of sodium nitrophenolate, 1-4 parts of indole butyric acid potassium, 5-15 parts of 5-nitrate and 10-20 parts of calcium nitrate. According to the invention, the liquid bacterial agent contains various high-activity strains including bacillus laterospor, bacillus licheniformis, bacillus subtilis and the like and dozens of high-purity nutritional ingredients. The liquid bacterial agent has super strong activity, and can quickly reach nutrient deficiency parts and bacteria infection parts of crops after being used so as to quickly complement the deficient elements, and can split mycelium and spore and remove soil-borne diseases so as to allow the crops to be quickly back to health. The liquid bacterial agent has the advantages of good water solubility, strong absorption ability, non-public hazard, no residue and no volatility after being used, and can not cause poison damage for the crops in the greenhouse.

The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid. To be specific, the present invention provides a process for producing a L-amino acid, comprising culturing a microorganism wherein the activity of the protein described in any one of (1) to (3) below is higher than that of the parent strain in a medium to produce the L-amino acid and accumulate the L-amino acid in the medium, and then collecting the L-amino acid from the medium:(1) a protein comprising the amino acid sequence shown by any one of SEQ ID NOS:2, 4, 6 and 8(2) a protein consisting of the amino acid sequence resulting from deletion, substitution or addition of one or more amino acids in the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity(3) a protein consisting of the amino acid sequence having 80% or more homology to the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity.

The invention discloses a novel process for treating fermentation waste gas of amino acid by utilizing a biofilter. Waste gas of zymotic fluid is subjected to Venturi dedusting and electrostatic dedusting, then fume containing extremely high COD and condensate are removed through a gas-liquid separator, and the condensate enters a sewage treatment system; and the fume enters the biofilter when the temperature is reduced to 35 DEG C through spraying, VOC in the fume is decomposed under the action of microorganisms, and gas which reaches the standard is exhausted from an exhaust funnel. The biofiltration and deodorization of the invention can treat certain specific malodorous gas, can also flexibly treat complicated mixed odor by converting biofiltration bed fillers and microbial strains, and have simple operation and no secondary pollution. The treatment process can greatly reduce the content of pollutants in the fume, is proved that the dehumidification rate and the removal rate of the volatile organic compounds can reach over 80 percent through a laboratory test, a pilot scale test and mass production, improve the living environment of surrounding residents, greatly reduce environment protection pressure, and has remarkable social benefit and environmental protection benefit.

The invention discloses a preparation method of a compound microbial fertilizer and the compound microbial fertilizer prepared by adopting the method. The preparation method comprises the steps that 30-70 parts of amino acid fermentation liquid waste with 30-70% of water content, 0-10 parts of urea, 3-8 parts of ammonium sulfate, 5-25 parts of monoammonium phosphate, 5-15 parts of potassium sulfate, 15-35 parts of potassium humate, 5-15 parts of stone powder, 10-20 parts of coal cinder, 0.5-2 parts of magnesium sulfate, 0.3-0.5 parts of borax, 0.5-1 part of zinc oxide and 5-10 parts of bentonite are granulated; the surfaces of the material granules are coated with composite microbial agents through a coating agent under the condition of the temperature lower than 60 DEG C. The preparation method utilizes the amino acid fermentation liquid waste containing rich micromolecular organic matter such as free amino acids, the surfaces of the material granules are coated with the composite microbial agents through the coating agent, the raw material costs can be reduced, and more abundant nutrients can be also provided. The survival rate of strains is improved, the problems of powder falling and loss and infection of mixed bacteria are decreased, and a better fertilizer effect is obtained.

The invention provides a liquid compound microbial fertilizer which comprises the following components in parts by weight: 1-10 parts of strain, 10-80 parts of amino acid fermentation waste liquid, 20-60 parts of inorganic fertilizer raw material, 5-10 parts of potassium fulvate, 1-5 parts of borax, 0.5-5 parts of EDTA-zinc, 0.05-0.5 part of aid, 0.1-4 parts of pH regulator, 0.1-1.5 parts of suspended thickener and 5-20 parts of water. The invention also provides a method for preparing the fertilizer. The fertilizer provided by the invention combines the advantages of the liquid fertilizer and the microbial fertilizer, and has comprehensive nutrients. After being applied, the fertilizer provided by the invention can reduce the consumption of the compound fertilizer, increase the fertilizer utilization ratio and enhance the crop yield and quality.

The invention discloses a novel process for treating fermented waste gas of amino acid by utilizing a spray cooling method. The fermented waste gas is conveyed to a gas-liquid separator by a fan, the separated wastewater with high COD enters a mash tank for recycling, gas enters a spray tower, and the condensed low-temperature gas enters the next working procedure through the upper part of the spray tower; the lower part of the spray tower is provided with a water tank, spray water subjected to temperature rise preliminarily filters solid purities obtained by the washing during the spraying in the water tank, then the filtered spray water enters a heat exchanger after the filtration through a filter to perform heat exchange with cooling water produced by a cooling tower, and the spray water is sent into the spray tower after the temperature of the spray water is reduced once again; and the produced condensed water and the spray water are mixed during the temperature reduction, then are drained out of the water tank, and are sent into a wastewater treatment plant. The process is simple, has convenient operation, ensures that the condensed water can be repeatedly used, has no problem of secondary pollution, and solves the technical problems of high water vapor content and large subsequent process load in the gas treated by the prior art.

The invention relates to a method for extracting L-valine by combining membrane separation and industrial chromatography separation, belonging to the field of biochemical engineering. The method comprises the steps: (1) thalli is removed by fermentation liquor; (2) inorganic salt and other amino acids are efficiently separated by industrial chromatography; (3) impurity and pigment are removed by the fermentation liquor; (4) the fermentation liquor is concentrated and crystallized, etc. The method has the characteristics of mild reaction condition, simple and convenient operation, less separating steps, good selectivity, clean production and the like, overcomes the defects of lower yield, large sewage discharge capacity and high production intensity in the prior art, and ensures the yield and quality of the L-valine to be obviously improved.

Methods of preparing a controlled release fertilizer include obtaining an amino acid fermentation byproduct liquor, and converting ammonium in the amino acid fermentation byproduct liquor to magnesium ammonium phosphate to obtain the controlled release fertilizer.

The invention belongs to the field of treatment of amino acid fermentation wastewater and discloses a method for producing liquid fertilizer by using glutamic acid wastewater. The method comprises the following steps: S1) filtering fermentation liquor; S2) decoloring, concentrating and crystallizing; S3) separating ammonium sulfate crystals; S4) hydrolyzing; and S5) preparing the fertilizer. According to the method, glutamic acid fermentation wastewater is utilized, so that the production cost of the fertilizer is greatly lowered, and wastes are turned into wealth. Moreover, the effect of the fertilizer is superior to that of marketed similar products.

The invention relates to a method for producing a microorganism luminous energy organic fertilizer by utilizing table / kitchen wastes. The method comprises the steps of: (1) carrying out solid-liquid separation on the table / kitchen wastes, deoiling the separated liquid, and inputting the separated solid ingredients into a grinder for grinding and then carrying out oxidative degradation; (2) inputting the deoiled liquid into a fermentation tank and inputting lactobacillus into the fermentation tank to carry out amino acid fermentation; (3) inputting the fermented mixture into a membrane filtration concentrator for concentration and then carrying out storage so as to generate a concentrated lactobacillus prepared fertilizer; (4) taking the concentrated lactobacillus and the oxidative degraded product as base materials, then mixing the base materials with an organic matter carrier and carrying out hydrolysis; (5) adding a photocatalyst to carry out nitrogen fixation and granulation; and (6) carrying out low-temperature drying to obtain the microorganism luminous energy organic fertilizer. According to the method provided by the invention, high-energy oxygen and microorganism lactobacillus are used for realizing the rapid hydrolysis, oxidation and degradation of organic biomasses in the table / kitchen wastes so as to generate the lactobacillus prepared fertilizer, so that the aims of harmlessly treating and recycling the organic biomass table / kitchen wastes are achieved.

The invention belongs to the technical field of fermentation, particularly relates to a method for preparing L-amino acid, and more particularly to a method for preparing L-amino acid by fermenting a cotton stalk hydrolysate. The method comprises the steps of inoculating fermentation strains for producing L-amino acid into a seed culture medium, and culturing to obtain seed solution, and then transferring the seed solution into a fermentation culture medium using the cotton stalk hydrolysate for replacing glucose as a carbon source, and fermenting. Compared with the prior art, the L-amino acid yield and conversion rate of using the cotton stalk hydrolysate as the carbon source for performing fermentation culture in the fermentation culture medium can achieve a level as the same as the level of fermentation using glucose as the carbon source; on one hand,by using the cotton stalk hydrolysate for replacing glucose as the carbon source, the problem of resource shortage can be alleviated, and moreover, a new way is opened up for resource utilization for cotton stalks, cyclic utilization for waste resources can be realized, and ecological environment can be protected, on the other hand, cotton stalks are agricultural wastes, low in cost, capable of effectively reducing production cost for L-amino acid, and good in industrialized application prospect.

The invention provides a method for extraction-separating an L-isoleucine by the combination of membrane separation and industrial chromatography separation, and belongs to the field of biochemical engineering. The method consists of the following steps: (1), removing thalli from a fermentation liquor; (2), separating inorganic salts and other amino acids in high efficiency by the industrial chromatography; (3), removing impurities and pigments from the fermentation liquor; and 4, condensing and crystallizing the fermentation liquor, and the like. The method has the characteristics of mild reaction conditions, easy operation, less separation steps, good selectivity, clean production and the like, and overcomes the defects of low yield, large discharge capacity of waste water and high production intensity existing in the prior art and obviously improves the yield and quality of the L-isoleucine.

The invention discloses a foliar preparation for improving crop resistance and a preparation method thereof, wherein the foliar preparation comprises an amino acid fermentation waste liquid, potassiumhumate, alginic acid, a macroelement fertilizer, a medium-trace element fertilizer, a surfactant, a plant growth regulator, a pH regulator and a suspension thickening agent. The foliar preparation ofthe invention adopts the amino acid fermentation waste liquid as the main organic component to realize waste liquid utilization, so that resources are saved, and the foliar preparation is environmentally friendly and safe. In addition, the amino acid fermentation waste liquid is rich in small molecular water-soluble organic matter, can chelate trace elements, can directly provide organic nutrition to plants, and promote nutrient absorption, thereby ensuring the accumulation of carbohydrates and nutrient absorption in plants in continuous rain and haze weather, maintaining healthy growth, andeffectively preventing occurrence of wilting, yellow leaves and the like of the plants, and therefore the cold resistance and disease resistance of crops are improved, and increase of the crop yield is facilitated.

The invention belongs to the technical field of amino acid fermentation extraction, and discloses a novel glutamic acid extracting process. The glutamic acid extracting process comprises the following steps: 1) weighing raw materials of a fermentation medium, 2) preparing the fermentation medium, 3) performing fermentation, 4) performing membrane filtration, and 5) performing evaporation concentration and isoelectric crystallization. By the glutamic acid extracting process provided by the invention, the used fermentation medium is low in cost, the conversion rate is high, the consumption of sulfuric acid and liquid ammonia is relatively low, and the industrial added value is increased.

Process for fluidized bed granulation of amino acid-containing fermentation broths comprising the stepsIntroduction of a drying gas with a temperature of 100° C.-450° C. into the fluidized bed granulation chamberSpraying of the amino acid-containing fermentation broth into the fluidized bed granulation chamberDischarge of the granules granulated in the fluidized bed granulation chamber with the drying gas stream, anddrying of the discharged granules in a fluidized bed drying step,wherein the discharged granulated granules are a granule mixture with various particle sizes and contains an oversize fraction, wherein the oversize comprises the particle sizes which lie above a desired particle size, an wherein the oversize fraction is removed from the discharged granule mixture and then comminuted and the comminuted oversize and the granule mixture from which the oversize was separated are fed into the fluidized bed drying step.

The invention relates to the field of amino acid fermentation and particularly discloses a preparation method of feed with low content of L-threonine. The preparation method comprises the steps of micro-filtering L-threonine fermentation liquid to obtain trapped liquid and filtration liquid, separating after concentration and crystallization on the filtration liquid so as to obtain mother liquid, mixing the trapped liquid with the mother iquid according to the volume ratio of 4:(2-3), concentrating the mixed liquid to 1 / 3-1 / 2 original volume so as to obtain a concentrated solution, adding corn protein accounting for 5-10% total weight of the concentrated solution, corn fiber accounting for 5-10% total weight of the concentrated solution and light calcium accounting for 1-3% total weight of the concentrated solution, and drying after mixing the mixed liquid to be uniform, thus obtaining the feed. The preparation method takes the trapped liquid and the mother liquid produced in the preparation technology of the feed with high content of L-threonine as materials and feed additives for adjuvant to prepare high-quality feed with low content of L-threonine, thereby solving the problem of environment pollution caused by the trapped liquid and the mother liquid and realizing zero emission of waste liquid.

A process is disclosed for the fermentative preparation of amino acids, in which an L-lysine producing bacterial strain of the species Corynebacterium glutamicum is cultivated in a nutrient medium and the amino acids can be isolated from the culture medium at the end of fermentation. After the vigorous growth phase, the bacterial culture has at its disposal a smaller quantity of assimilable carbon source than it could metabolize on the basis of the structure of the strain and the quanitity of other necessary supplements provided in the nutrient medium.

The invention relates to the field of amino acid fermentation waste water treatment, in particular to a biological agent used for treating high-concentration waste water produced by amino acid fermentation. The preparation method of the biological agent includes 1, preparing composite bacteria solution; 2, preparing composite algae solution; 3, preparing a biological carrier; 4, preparing the biological agent. The biological agent has the advantages of high waste water treating efficiency, low usage and promised application prospect.

The invention discloses a method for constructing a corynebacterium constitutive expression vector promoter based on transcriptome sequence. The invention further discloses the corynebacterium constitutive expression vector promoter constructed based on the transcriptome sequence, an expression vector containing the promoter and a recombination strain obtained by converting host cell corynebacterium glutamicum by the expression vector. According to the expression vector constructed by the promoter obtained by the method, the target gene protein expression quantity of the logarithmic phase is low, but the target gene protein expression quantity of the stable phase is greatly improved relative to the logarithmic phase, and the effect to the host bacteria growth is low, and the stability of the plasmid passage is high. The expression vector is suitable for constructing the vector of the target gene of which the logarithmic phase does not need the expression, but the stable phase needs the high expression, and is particularly suitable for constructing engineered strains for amino acid fermentation.

Up to now, the perfect treatment of amino acid fermenting effluent has become the key for amino acid producing enterprise to develop, and the present invention is one perfect technological process of treating amino acid fermenting effluent. The technological process includes the main steps of concentrating, spraying slurry to pelletize, washing and dedusting, electrostatic treating, biochemical treating, etc. and converts the fermented effluent into valuable compound fertilizer, clean water and gas. For the first time, the technological process adopts a sweeping, catching and vapor chromatography-mass spectrometry method for determining the components of pelletizing tail gas identified as aerosol; develops electrostatic technology and apparatus for treating the slurry spraying second pelletizing tail gas; and develops the electrostatic treatment to enrich high concentration organic effluent.

The invention provides a fertilizer for improving the high temperature and cloudy or rainy day adaptability of plants. Main components of the fertilizer comprise 1-20wt% of elemental nitrogen, 1-20wt% of elemental phosphorus, 1-20wt% of elemental potassium, 40-65wt% of an amino acid fermentation liquid, 1-8wt% of trace elements, 1-5wt% of a plant nutrition regulator and 1-5wt% of a microbial agent. The trace elements comprise 0.1-1% of elemental zinc, 0.8-5% of elemental magnesium and 0.1-2% of elemental manganese. The amino acid fermentation liquid adopted as a main raw material is rich in micro-molecular organic matters, amino acids, plant regulators and other active substances and trace elements, so when crops encounter high temperature, high humidity, cloudy low light and other adversity stress, roots, stems and leaves can rapidly absorb the active substances to supplement carbon sources needed by breathing, reinforce the fixation ability of carbon dioxide and promote photosynthesis. Synergism of the active substances and beneficial microbes makes growth of harmful pathogens in soil be effectively inhibited, soil borne diseases be prevented and treated, damages of the adversity stress to the crops be alleviated and yield reduction and loss of the crops be alleviated.

The invention relates to a special liquid package cotton fertilizer for improving soil, and a preparation method and application method of the liquid package fertilizer. According to the special liquid package cotton fertilizer for improving soil, the special liquid package fertilizer consists of a composite microbial liquid fertilizer and a macroelement water-soluble fertilizer, wherein the composite microbial liquid fertilizer consists of an amino acid fermentation liquid, fulvic acid, microbial bacteria, a nitration inhibitor and polyaspartic acid, and the macroelement water-soluble fertilizer consists of urea, monoammonium phosphate, potassium chloride, magnesium sulfate heptahydrate, EDDHA-iron, EDDHA-zinc, EDDHA-manganese, EDDHA-copper, boric acid, carboxymethyl cellulose, an amino acid fermentation liquid, bentonite and sodium pyrophosphate. The invention also discloses the preparation method and application method of the package fertilizer. According to the special liquid package cotton fertilizer for improving the soil provide by the invention, the requirements on nutrients in different growth and development periods of cotton can be met, and salinization of soil in Xinjiang can be improved. The application method of the special liquid package fertilizer conforms to the growth characteristics and fertilizer requirement laws of the cotton.

The invention relates to the field of amino acid fermentation, and specifically discloses a preparation method of corn steep liquor. According to the preparation method of the invention, corn kernels are soaked in sulphurous acid, concentration is carried out to obtain a corn steep liquor, microfiltration is performed on the corn steep liquor by using a filter membrane having a pore size of 0.05-0.1mum until the concentration factor is 10-12 to obtain a filtrated liquid and a retentate, and the filtrated liquid is concentrated until a baume degree of 8-24 is achieved so as to obtain the corn steep liquor. According to the invention, the microfiltration is performed on the corn steep liquor before the preparation of the corn steep liquor, microorganisms which cause contamination with corn steep liquor fermentation are trapped, the prepared corn steep liquor can be used in the amino acid fermentation after undergoing high temperature sterilization, contamination degree of the corn steep liquor used in the amino acid fermentation is greatly reduced, contamination rate is reduced, and acid yield level of amino acid is increased at the same time.

The invention relates to an environment-friendly snow-melting agent and a method for preparing the snow-melting agent by using an amino acid fermentation liquid. The main components of the snow-melting agent include organic acid ammonium salt, amphoteric amino acid ammonium sulfate salt, ammonium sulfate and urea. The method comprises the following steps: using a residual liquid after extracting amino acid from the amino acid fermentation liquid or whey protein hydrolysate as a raw material; firstly conducting concentrating; then conducting decolorization of the concentrated solution; conducting filtration; adding the urea in the obtained filtrate; neutralizing a product with ammonium hydroxide until pH of the product is 7-8 to obtain a viscous fluid; conducting spraying granulation with a fluidized bed to obtain the snow-melting agent. The snow-melting agent has low snow melting temperature, is easy to dissolve and has zero damage to the environment, the biodegradation is complete, and the problem of soil salinization caused by a conventional inorganic snow-melting agent is avoided. The preparation method is simple to operate and low in cost.