Fertilizer prepared by utilizing threonine fermented wastes
A technology for fermenting waste and threonine fermentation liquid, which is applied in the directions of ammonium orthophosphate fertilizer, alkaline orthophosphate fertilizer, organic fertilizer, etc. The effect of reducing production cost, reducing raw material cost and improving utilization rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] A kind of fertilizer that utilizes threonine to ferment waste to prepare, it comprises the following steps:
[0025] Step 1) preparation of threonine fermentation broth, step 2) filtration and decolorization, step 3) separation of crystals and mother liquor, step 4) hydrolysis of bacterial protein, step 5) preparation of feed liquid A, and step 6) preparation of fertilizer;
[0026] 1) Preparation of threonine fermentation broth: Escherichia coli engineering bacteria K12△dapA (see "Optimization of E. (Refer to documents such as CN1305009A and CN1284560A) The mixed bacterial solution is put into the seed tank according to 10% inoculation amount for cultivation, at a temperature of 32°C and a shaker speed of 180r / min, cultivated for 12 hours to obtain liquid A; then according to the liquid A: The fermentor culture medium is that the volume ratio of 1: 10 is transferred in the fermentor and cultivated, and temperature is 32 ℃, cultivates 5 days, obtains fermented liquid; T...
Embodiment 2
[0034] A kind of fertilizer that utilizes threonine to ferment waste to prepare, it comprises the following steps:
[0035] Step 1) preparation of threonine fermentation broth, step 2) filtration and decolorization, step 3) separation of crystals and mother liquor, step 4) hydrolysis of bacterial protein, step 5) preparation of feed liquid A, and step 6) preparation of fertilizer;
[0036] 1) Preparation of threonine fermentation broth: Escherichia coli engineering bacteria K12△dapA (see "Optimization of E. (Refer to documents such as CN1305009A and CN1284560A) The mixed bacterial solution is put into the seed tank according to the inoculum amount of 5% for cultivation, at a temperature of 32°C, with a shaker speed of 200r / min, and cultivation for 16 hours to obtain liquid A; then, according to the liquid A: the fermentor culture medium is that the volume ratio of 1: 10 is transferred in the fermentor and cultivated, and the temperature is 32 ℃, cultivates for 5 days, obtains fe...
Embodiment 3
[0044] Fertilizer effect test of fertilizer of the present invention
[0045] 1. Fertilizer experiment for planting sorghum:
[0046] The control group used compound fertilizer (nitrogen-phosphorus-potassium=23-7-6); the experimental group used the fertilizer prepared in Example 1 of the present invention; the soil quality and planting conditions of the test field were exactly the same, and the area was one mu; the amount of fertilizer applied was 80kg / mu. The specific test results are shown in Table 1:
[0047] Table 1
[0048] group Yield per mu (Kg) Production increase (%) Fertilization input (yuan) control group 487.9 / 208 test group 523.1 7.21 168
[0049] Note: The fertilizer for the control group is calculated at 2.6 yuan / kg, and the fertilizer for the experimental group is calculated at 2.1 yuan / kg.
[0050] Second, planting sweet potato fertilizer experiment:
[0051] Set up two treatment test fields, each with an area of on...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com