Method for extracting and separating L-valine by membrane separation and plant chromatography separation

Abstract

The invention relates to a method for extracting L-valine by combining membrane separation and industrial chromatography separation, belonging to the field of biochemical engineering. The method comprises the steps: (1) thalli is removed by fermentation liquor; (2) inorganic salt and other amino acids are efficiently separated by industrial chromatography; (3) impurity and pigment are removed by the fermentation liquor; (4) the fermentation liquor is concentrated and crystallized, etc. The method has the characteristics of mild reaction condition, simple and convenient operation, less separating steps, good selectivity, clean production and the like, overcomes the defects of lower yield, large sewage discharge capacity and high production intensity in the prior art, and ensures the yield and quality of the L-valine to be obviously improved.

Description

technical field [0001] The invention relates to a method for extracting and separating L-valine, in particular to a method for separating and extracting L-valine from a fermentation liquid by using a combination technology of membrane separation and industrial chromatographic separation. It belongs to the field of biochemical industry. Background technique [0002] As one of the branched-chain amino acids, L-valine is an essential amino acid for the human body and has various physiological functions. Because of its special structure and function, it occupies a particularly important position in the metabolism of human life. It is mainly used to prepare compound amino acid infusions, synthetic polypeptide drugs and food antioxidants, etc., especially in medical research and treatment, and is increasingly valued. . It is used in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic defects, tr...

AI Technical Summary

Problems solved by technology

The general process of this process is: the fermentation broth is filtered to remove the bacteria, and the separation and extraction of L-valine from the fermentation broth using this process has the following problems: one is to use the method of filtration to remove the bacteria, because the bacteria are small, usually the bacteria The removal is not complete; the second is that the color value of the L-valine fermentation liquid is relatively high, and the decolorization of ordinary activated carbon is not complete and the amount is large, which affects the product quality and yield; the third is that there are many impurities and high content in the L-valine fermentation liquid , direct use of ion exchange method, the resin is seriously polluted, it is easy to cause the resin structure to be broken and lose the regeneration ability, which increases the operating cost. major issues

Membrane separation technology has been used in the separation and purification of fermentation products. There have been successful precedents, but there is no report on the separation and extraction of L-isoleucine from fermentation broth using membrane separation and industrial chromatographic separation technology.