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Method for preparing L-amino acid

A technology of amino acids and seeds, applied in the field of fermentation, can solve problems such as shortage of raw material resources and rising prices, and achieve the effects of alleviating resource shortage, realizing recycling, and reducing production costs

Active Publication Date: 2013-11-20
MEIHUA BIOTECH LANGFANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to provide a method for preparing L-amino acid in view of the shortage of raw material resources and rising prices faced by the existing amino acid fermentation industry, using cotton stalk hydrolyzate instead of glucose in the fermentation medium for fermentation , can reach the same level as fermenting with pure glucose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Inoculate the activated L-glutamic acid-producing bacterium Brevibacterium flavum ATCC14067 in the sterilized shake flask seed medium, the seed medium formula is: glucose 20g / L, corn steep liquor 10g / L, soybean cake hydrolyzate 10g / L, dipotassium hydrogen phosphate 3g / L, urea 5g / L, pH7.0-7.2; placed in a reciprocating shaker at 32°C and 150rpm for shaking culture, cultured for 12h and then taken out.

[0046] According to the 10% inoculation amount, the seed culture solution was inserted into the sterilized shake flask fermentation medium, and the fermentation medium formula was: glucose 80g / L, corn steep liquor 5g / L, bean cake hydrolyzate 5g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1g / L, molasses 1g / L, pH7.0-7.2. The different shake flasks are calculated according to the glucose content in the cotton stalk hydrolyzate, and the cotton stalk hydrolyzate is used to replace the glucose in the fermentation medium at a ratio of 10% to 100%, and placed in...

Embodiment 2

[0052] The activated L-lysine-producing strain Corynebacterium glutamicum FREM-P1709 was inoculated in the sterilized shake flask seed medium, and the seed medium formula was: glucose 30g / L, corn steep liquor 15g / L, Ammonium sulfate 10g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, pH 7.2; placed in a reciprocating shaker at 30°C and 150rpm for shaking culture, cultured for 12 hours and then taken out.

[0053] According to the 10% inoculation amount, the seed culture solution was inserted into the sterilized shake flask fermentation medium, and the fermentation medium formula was: glucose 160g / L, ammonium sulfate 50g / L, corn steep liquor 15g / L, magnesium sulfate 0.4g / L L. Dipotassium hydrogen phosphate 1g / L, molasses 10-20g / L, soybean meal hydrolyzate 35g / L, calcium carbonate 50g / L, pH7.0-7.2. Wherein in the shaking flask of the replacement group, the glucose in the fermentation medium is replaced by cotton stalk hydrolyzate according to the glucose equiva...

Embodiment 3

[0055] Inoculate the activated L-tryptophan-producing bacterium Corynebacterium glutamicum FREM-P7128 in the sterilized shake flask seed medium, the seed medium formula is: glucose 30g / L, corn steep liquor 40g / L, Ammonium sulfate 5g / L, soybean meal hydrolyzate 20g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.4g / L, manganese sulfate 0.01g / L, ferrous sulfate 0.01g / L, vitamin B 1 300 μg / L, vitamin H 200 μg / L, pH 7.2; placed in a reciprocating shaker at 32°C and 150 rpm for shaking culture, cultured for 16 hours and then taken out.

[0056] According to the 10% inoculation amount, the seed culture solution was inserted into the sterilized shake flask fermentation medium. The fermentation medium formula was: glucose 100g / L, ammonium sulfate 40g / L, corn steep liquor 22g / L, soybean meal hydrolyzate 25g / L. L, magnesium sulfate 0.4g / L, potassium dihydrogen phosphate 10g / L, manganese sulfate 0.01g / L, ferrous sulfate 0.01g / L, vitamin B 1 100μg / L, vitamin H50μg / L, pH7.2. ...

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Abstract

The invention belongs to the technical field of fermentation, particularly relates to a method for preparing L-amino acid, and more particularly to a method for preparing L-amino acid by fermenting a cotton stalk hydrolysate. The method comprises the steps of inoculating fermentation strains for producing L-amino acid into a seed culture medium, and culturing to obtain seed solution, and then transferring the seed solution into a fermentation culture medium using the cotton stalk hydrolysate for replacing glucose as a carbon source, and fermenting. Compared with the prior art, the L-amino acid yield and conversion rate of using the cotton stalk hydrolysate as the carbon source for performing fermentation culture in the fermentation culture medium can achieve a level as the same as the level of fermentation using glucose as the carbon source; on one hand,by using the cotton stalk hydrolysate for replacing glucose as the carbon source, the problem of resource shortage can be alleviated, and moreover, a new way is opened up for resource utilization for cotton stalks, cyclic utilization for waste resources can be realized, and ecological environment can be protected, on the other hand, cotton stalks are agricultural wastes, low in cost, capable of effectively reducing production cost for L-amino acid, and good in industrialized application prospect.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a method for preparing L-amino acid, in particular to a method for preparing L-amino acid by fermentation of cotton stalk hydrolyzate. Background technique [0002] Amino acid refers to carboxylic acid containing amino group, which is the basic unit of biomacromolecular protein synthesis. It endows the protein with a specific molecular structure and makes its molecules biochemically active. Amino acids are closely related to the life activities of organisms. play an important role in the metabolic process. Amino acids are widely used in industries such as food, medicine, feed and cosmetics, and the amino acid industry is one of the pillar industries in the national economy. [0003] There is an amino group on the carbon atom directly connected to the carboxyl group in the amino acid. The groups or atoms connected to the carbon atom are different. It is called a chiral carb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12P13/14C12P13/08C12P13/22C12P13/06C12P13/10C12R1/13C12R1/15
Inventor 李必金于宏艳叶华龚华
Owner MEIHUA BIOTECH LANGFANG CO LTD
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