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Preparation method of feed with low content of L-threonine

A threonine, low-content technology, applied in the field of amino acid fermentation, can solve the problems of high COD, waste COD (high chemical oxygen consumption, environmental pollution, etc.), and achieve the effect of zero discharge of waste liquid.

Active Publication Date: 2012-07-18
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current process will produce retentate and mother liquor in the stage of microfiltration and concentration and crystallization respectively. Among these two waste liquids, the retentate contains a large amount of bacteria, and the mother liquor contains a large amount of organic matter such as pigments and soluble proteins, resulting in The COD (chemical oxygen demand) of the waste liquid is high, and the mother liquor contains a small amount of sulfate, chloride and ammonium salt
However, the existing process generally discharges waste liquid without treatment, causing serious pollution to the environment.
Even if there is an environmental protection process to treat the waste liquid, the effect is not satisfactory, and the COD in the treated waste water is still high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: preparation method of the present invention

[0018] Take L-threonine fermentation broth 60m 3 Microfiltration through a ceramic membrane (0.3μm pore size) at 40°C, the concentration factor is 8 times, and 52.5m 3 Filtrate and 7.5m 3 The retentate; the filtrate was concentrated and crystallized at 60°C to obtain 10.5m 3 Concentrate.

[0019] The concentrated solution was cooled to 10°C and centrifuged to obtain 5.6m 3 Mother liquor and 6200kg L-threonine wet crude product, the wet crude product is further refined into high-content L-threonine feed, the yield is 75%, and the purity is 98.6%.

[0020] Put 7.5m 3 Retentate, 5.6m 3 The mother liquor is mixed and concentrated to 6.5m at 60°C with a vacuum degree of ≤-0.1Mpa using double effects 3 .

[0021] The 6.5m 3 The concentrated solution is spray-dried, and the drying temperature is controlled at 100° C. to obtain 3.5 t of L-threonine feed, the content of L-threonine is 65%, the crude protein is ...

Embodiment 2

[0022] Embodiment 2: preparation method of the present invention

[0023] Take L-threonine fermentation broth 60m 3 Microfiltration through a ceramic membrane (0.5μm pore size) at 40°C, the concentration factor is 9 times, and 53.2m 3 Filtrate and 6.8m 3 The retentate; the filtrate was concentrated and crystallized at 60°C to obtain 9m 3 Concentrate.

[0024] The concentrated solution was cooled to 10°C and centrifuged to obtain 3.4m 3 Mother liquor and 6600kg L-threonine wet crude product, the wet crude product is further refined into high-content L-threonine feed, the yield is 80%, and the purity is 98.2%.

[0025] Put 6.8m 3 Retentate, 3.4m 3 The mother liquor is mixed and concentrated to 4.1m at 60°C with a vacuum degree of less than -0.1Mpa using double effects 3 .

[0026] The 4.1m 3 The concentrated solution is spray-dried, and the drying temperature is controlled at 100° C. to obtain 2.5 t of L-threonine feed, the content of L-threonine is 60%, the crude prote...

Embodiment 3

[0027] Embodiment 3: preparation method of the present invention

[0028] Take L-threonine fermentation broth 60m 3 Microfiltration through a ceramic membrane (0.1 μm pore size) at 40°C, the concentration factor is 8.5 times, and 52.9m 3 Filtrate and 7.1m 3 The retentate; the filtrate was concentrated and crystallized at 65°C to obtain 10.6m 3 Concentrate.

[0029] The concentrated solution was cooled to 10°C and centrifuged to obtain 5.3m 3 Mother liquor and 6400kg L-threonine wet crude product, the wet crude product is further refined into high-content L-threonine feed, the yield is 77%, and the purity is 98.5%.

[0030] Put 53m 3 L-threonine fermentation broth, 7.1m 3 Retentate, 5.3m 3 The mother liquor is mixed (concentrated to 26.2m at 60°C with a vacuum degree of ≤-0.1Mpa using double-effect 3 .

[0031] The 26.2m 3 The concentrate was spray-dried, and the drying temperature was controlled at 100° C. to obtain 13.1 t of L-threonine feed, with an L-threonine con...

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PUM

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Abstract

The invention relates to the field of amino acid fermentation and particularly discloses a preparation method of feed with low content of L-threonine. The preparation method comprises the steps of micro-filtering L-threonine fermentation liquid to obtain trapped liquid and filtration liquid, separating after concentration and crystallization on the filtration liquid so as to obtain mother liquid, mixing the trapped liquid with the mother iquid according to the volume ratio of 4:(2-3), concentrating the mixed liquid to 1 / 3-1 / 2 original volume so as to obtain a concentrated solution, adding corn protein accounting for 5-10% total weight of the concentrated solution, corn fiber accounting for 5-10% total weight of the concentrated solution and light calcium accounting for 1-3% total weight of the concentrated solution, and drying after mixing the mixed liquid to be uniform, thus obtaining the feed. The preparation method takes the trapped liquid and the mother liquid produced in the preparation technology of the feed with high content of L-threonine as materials and feed additives for adjuvant to prepare high-quality feed with low content of L-threonine, thereby solving the problem of environment pollution caused by the trapped liquid and the mother liquid and realizing zero emission of waste liquid.

Description

technical field [0001] The invention relates to the field of amino acid fermentation, and specifically provides a preparation method of low-content L-threonine feed. Background technique [0002] Threonine, whose chemical name is α-amino-β-hydroxybutyric acid, has four stereoisomers, but only the L-form is biologically active. L-threonine is an essential amino acid, mainly used in medicine, chemical reagents, food fortifiers, feed additives, etc., especially the rapid growth of the amount of feed additives, it is often added to the feed of young piglets and poultry Among them, is the second limiting amino acid for pig feed and the third limiting amino acid for poultry feed. Experiments have shown that L-threonine is necessary for animals to maintain growth. Animals cannot synthesize it by themselves and must be supplied by food. L-threonine deficiency can lead to reduced animal feed intake, stunted growth, reduced feed utilization, and immunosuppressive symptoms. Therefor...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/175
Inventor 张学利王海雷彭芳菊党银博钟秀如龚华
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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