34 results about "Aequorea aequorea" patented technology

Isoforms of apoaequorin and isoforms of aequorin are isolated and purified from recombinant apoaequorin and a solution containing regenerated aequorin, respectively, by gradient elution chromatography. As a result, aequorin can be isolated and purified.

The present invention includes a fluorescent compound that can detect an activity, such as an enzymatic activity, and exhibits quenching. The fluorescent compound can include a fluorescent protein, such as an Aequorea-related green fluorescent protein. The fluorescent compound can include a substrate site for an enzymatic activity such as a kinase activity, a phosphatase activity, a protease activity, and a glycosylase activity. The fluorescent compound of the present invention can be used to detect such enzymatic activities in samples, such as biological samples, including cells. The present invention also includes nucleic acids that encode the fluorescent compounds of the present inventions, and cells that include such nucleic acids or fluorescent compounds.

The present invention relates to a method for separating collagen from jellyfish by using radiation. More precisely, acid-soluble collagen and attelo collagen were prepared in this invention by using the method combining irradiation technique and chemical treatment. This method of the invention is expected to be useful for the separation of collagen from jellyfish with low costs but high yield.

The invention relates to an anti-wrinkle moisturizing eye mask and a preparation method thereof, and belongs to the technical field of eye masks. The anti-wrinkle moisturizing eye mask comprises an eye mask liquid, wherein the eye mask liquid is composed of 1.5-4.5 parts by weight of a radix ginseng extract, 1-3 parts by weight of retinol, 3-7 parts by weight of resveratrol, 1.5-4.5 parts by weight of a jellyfish extract, 1.5-4.5 parts by weight of a citrus reticulata fruit extract, 1-3 parts by weight of arbutin, 3-7 parts by weight of papain, 0.5-1.5 parts of a polypeptide-1, 1-3 parts of trehalose, 3-7 parts by weight of a green tea extract, 3-7 parts by weight of a pearl powder, 1.5-4.5 parts by weight of a herba menthae heplocalycis extract, 1-3 parts by weight of an edible bird nestextract, 1-3 parts by weight of a radix scutellariae extract, and 1.5-4.5 parts by weight of propolis. The eye mask liquid of the anti-wrinkle moisturizing eye mask has good permeability, is easy to absorb and makes the eye mask have anti-wrinkle and moisturizing effects; and the pure natural traditional Chinese medicinal herbs are adopted, and thus the anti-wrinkle moisturizing eye mask has no heavy metals and no toxic and side effects.

The present invention provides mutants of the Green Fluorescent Protein (GFP) of Aequorea victoria. Specifically provided by the present invention are nucleic acid molecules encoding mutant GFPs, the mutant GFPs encoded by these nucleic acid molecules, vectors and host cells comprising these nucleic acid molecules, and kits comprising one or more of the above as components. The invention also provides methods for producing these mutant GFPs. The fluorescence of these mutants is observable using fluorescein optics, making the mutant proteins of the present invention available for use in techniques such as fluorescence microscopy and flow cytometry using standard FITC filter sets. In addition, certain of these mutant proteins fluoresce when illuminated by white light, particularly when expressed at high levels in prokaryotic or eukaryotic host cells or when present in solution or in purified form at high concentrations. The mutant GFP sequences and peptides of the present invention are useful in the detection of transfection, in fluorescent labeling of proteins, in construction of fusion proteins allowing examination of intracellular protein expression, biochemistry and trafficking, and in other applications requiring the use of reporter genes.

The invention discloses a preparation process of a jellyfish droplet ceramic product, which comprises the following steps: preparing blank mud and glaze slip, and carrying out throwing molding, fettling, biscuiting and cooling on the blank mud to obtain a blank body; pouring the glaze slip into the green body, staying for 25-30 seconds, pouring out the glaze slip, airing, reversely buckling the green body into the glaze slip, soaking for 25-30 seconds, and not glazing and airing the outer bottom of the green body; and putting the glazed and air-dried green body into an electric kiln for roasting, and taking out of the kiln. A reasonable formula is obtained through scientific proportioning, an innovative firing mode is adopted, finally the jellyfish droplet ceramic product is obtained, theglaze bottom color of the ceramic product is black, oil drops are silver blue jellyfish drops, and the Jellyfish droplet ceramic product is hidden, transparent, different in size, flexible, fat and smooth in glaze and small in number of needle holes.

The invention provides a jellyfish growth promoting peptide and a preparation method thereof. The preparation method of the jellyfish growth promoting peptide comprises the following steps: a fermentation step: inoculating a mixed strain into a slurry containing jellyfish tentacle particles, and carrying out fermentation culture to obtain a fermentation broth; a separation step: carrying out separation treatment on the fermentation broth to obtain a separation product containing the jellyfish growth promoting peptide; wherein the mixed strain comprise two or a combination of more than two of lactobacillus acidophilus, lactobacillus bulgaricus, bifidobacterium, pseudoalteromonas, candida utilis, saccharomyces cerevisiae, bacillus natto and bacillus subtilis. The jellyfish growth promoting peptide is more suitable in molecular weight, high in safety, excellent in stability and high in transdermal absorption rate. Furthermore, the jellyfish growth promoting peptide disclosed by the invention has a good promoting effect on growth or proliferation of cells in the skin, and can be used for improving the regeneration capacity of the cells in the skin and improving the elasticity of the skin.

A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).

The invention discloses a preparation method of jellyfish active protein. The preparation method comprises the following steps: 1, preparation of collagen or collagen sponge: extracting collagen or collagen sponge from jellyfish; and 2, dissolving collagen or collagen sponge in a high-concentration urea solution to obtain the collagen sponge. The invention further discloses jellyfish active protein and application thereof. According to the invention, after the collagen sponge is extracted, high-concentration urea is added for denaturation, so that insoluble tissues or cell debris and lipid substances which are co-precipitated can be separated.

The present invention provides a crystal mask prepared with jellyfish water. The crystal mask is made of jellyfish water, a film-forming substrate and a product preservative as raw materials. A preparation method of the mask is as follows: 1) extracting jellyfish water: taking fresh or frozen jellyfish, removing tentacles and endoderm, washing the jellyfish clean with purified water, crushing thewashed jellyfish, adding protease, conducting enzymatic reaction at 30-60 DEG C for 6-10 hours, conducting filtering to remove residues, and collecting a filtrate, namely extracted jellyfish water; and 2) preparing crystal mask: using the jellyfish water extracted in the step 1) as a water agent, adding a film-forming substrate and a product preservative, heating the mixture to 80-100 DEG C, stirring the mixture until completely dissolved, conducting heat-preservation still-putting at 80 DEG C and de-foaming, pouring the mixture into a mold when still hot, and after conducting cooling and molding, conducting de-molding and packaging to form a colorless and transparent jelly-shaped crystal mask. The crystal mask has the following technical effects: the prepared crystal mask has excellent moisturizing and skin-whitening effects.

The present invention relates to the use of a supramolecular system in order to remove particles from a liquid medium containing same. According to the invention, the supramolecular system includes at least one molecule having a low molecular weight and / or an organic compound from living organisms, preferably from jellyfish, said compound being selected from among collagen, a polysaccharide, a proteoglycan or a mixture of two of said organic compounds, and said molecule having a low molecular weight and formula (I) as defined herein. The invention also relates to a method for removing particles from a liquid medium containing same. The invention is particularly suitable for use in water decontamination and biotechnology.

The invention discloses a zooplankter breeding circulating system with a bionic ctenophore structure, and belongs to the field of marine organism breeding equipment. The system comprises a breeding tank, an incubator, water outlet pipes and water inlet pipes; the breeding tank is transparent and spherical; the lower ends of the two water outlet pipes are arranged in the incubator; the water inlet pipes comprise a main water inlet pipe, branch water inlet pipes and secondary branch water inlet pipes; the upper part of the main water inlet pipe is arranged in the breeding tank, the bottom of the main water inlet pipe extends into the middle upper part in the incubator, the upper ends of the four branch water inlet pipes are connected with four branch water outlet holes respectively, the lower end of each branch water inlet pipe is respectively branched to form the two secondary branch water inlet pipes, and the secondary branch water inlet pipes are fixed to the inner side surface of the breeding tank, and a plurality of water outlet holes are formed in the secondary branch water inlet pipes. The system is applied to the aspect of zooplankter breeding, solves the problem that an existing jellyfish breeding device for hydranth attachment cannot be used for breeding zooplankter such as ctenophore and the like, and has the characteristics that bionic and automatic circulation are integrated, the requirement for breeding the zooplankter with weak swimming capacity is met and microscopic display and science and education propaganda are integrated.

The invention discloses a jellyfish toxin polypeptide, belongs to the field of biological chemistry and molecular biology and particularly relates to a jellyfish toxin polypeptide, preparation and application. The amino acid sequence is a bran-new sequence. The polypeptide is synthesized chemically; or a gene recombination method is adopted for expressing polynucleotide capable of coding the polypeptide, and the polypeptide is obtained. The jellyfish toxin polypeptide is used for anesthesia and pain easing in operations. The jellyfish toxin polypeptide, preparation and application have the advantages that the jellyfish toxin polypeptide is used for general anesthesia and pain easing in operations, does not act on an opiate receptor and is free of addiction.

The invention relates to a jellyfish mucin separation method and application thereof in repairing cosmetics. High-purity and high-viscosity mucin is separated from the mesoglea of a jellyfish by utilizing the difference of isoelectric points of the mucin and collagen and combining ethanol fractional precipitation; and clinical tests on human bodies show that the mucin can repair damaged skin barriers and reduce skin sensitivity. The method comprises the following steps of: rinsing a jellyfish, cutting the jellyfish open from the top end of an umbrella part, and digging out mesoglea jelly; homogenizing the jelly, and putting homogenate into an ultrasonic extraction kettle to be ablated to obtain ablated mucus; adjusting the pH value of the ablated mucus to 5-8, and adding ethanol to make the ethanol to reach 10-40%; performing primary centrifugation, and collecting supernatant of the primary centrifugation; adjusting the pH value of the supernatant of the primary centrifugation to 2-4,and adding ethanol to make the ethanol to reach 40-70%; performing secondary centrifugation, collecting precipitate, and rinsing the precipitate with acidic ethanol; and redissolving the precipitate with buffer solution, and performing freeze-drying to obtain the white cotton mucin.

The invention relates to a skin spray based on deep repair and long-lasting moisture preservation. The skin spray is prepared from antarctic ice brown alga extract, auroral alga extract, arctic draparnaldia extract, jellyfish extract, sea spring water, hydrolyzed lupin protein, fragrant citrus fruit extract, 1,3-propanediol, poly-gamma-sodium glutamate, beta-sitosterol sodium sulfate, tocopheryl linoleate, wild soybean seed extract and other raw materials. Compared with the prior art, the skin spray has the advantages that the varieties and use quantities of the raw materials are strictly regulated, so that the technical formula of replenishing water to the surface layer (cuticle), replenishing nutrients (lipopolysaccharide, protein, microelements and the like) to the deep layer and activating cells meets the skin moisture preservation mechanism better, and the optimized formula design of the ingredients with the moisture preservation barrier and repair synergism is achieved.

The invention relates to the field of biological medicines, in particular to application of epigallocatechin gallate (EGCG) in preparation of a medicine for preventing or treating systemic poisoning of jellyfish toxin. The invention provides a new application of EGCG, injection administration of EGCG can play an obvious role in preventing and treating a jellyfish toxin systemic poisoning animal model, and experimental results show that injection administration of EGCG can effectively prevent and treat jellyfish toxin systemic poisoning, so that an effective candidate drug is provided for developing a drug for preventing or treating jellyfish stings systemic poisoning, and the EGCG can be applied to prevention and treatment of jellyfish stings systemic poisoning. And reference is provided for clinical research of new application of the medicine.

The present disclosure relates to the technical field of biomedicine, and provides an anti-physaliatoxin nanobody, and a preparation method and use thereof. The nanobody is a VHH antibody with an amino acid sequence shown in SEQ ID NO. 1. Affinity analysis shows that the nanobody of the present disclosure has prominent affinity. It is proved by small animal experiments that, after mice in an antibody protection group pre-injected with the nanobody of the present disclosure are injected with physaliatoxin, no mice shows toxic symptoms, and during continuous observation for one month, no toxic lethality occurs, indicating that the nanobody of the present disclosure shows excellent anti-physaliatoxin effects, excellent preventive or therapeutic effects on jellyfish stings, and promising clinical application prospects.

The invention discloses a preparation method of a cosmetic additive based on dendrobium extract. According to main points of a technical scheme in the invention, the preparation method comprises the following steps: S11, material preparation: preparing the raw materials consisting of in parts by weight, 5-10 parts of dendrobium nobile extract, 2-5 parts of a secret liquid, 1-3 parts of composite vitamins, 0.5-0.8 part of schizophyllan, 1-3 parts of a jellyfish extract, 1-3 parts of plant polysaccharides and 1-5 parts of deionized water; S12, primary mixing: sequentially adding the plant polysaccharides, the schizophyllan, the composite vitamins and the deionized water into a stirrer, conducting heating to 65-75 DEG C in a water bath, and fully conducting stirring to obtain a mixed solution A; and S13, secondary mixing: putting the dendrobium nobile extract and the jellyfish extract into the stirrer, performing water bath heating to 55-65 DEG C, and performing sufficient stirring to obtain a mixed solution B. The prepared cosmetic additive has the advantages of improving skin quality, repairing cuticle, delaying cell aging, improving skin immunity, removing freckles, inhibiting acnes, deeply supplementing moisture and preserving moisture.

The invention discloses a jellyfish whisker inspired discrete structure color microsphere implanted fiber and a preparation method and application thereof, and the preparation method comprises the following steps: step 1, preparing a co-flow micro-fluidic chip comprising an internal phase capillary tube and an external phase capillary tube; step 2, preparing discrete structural color hydrogel microspheres by using a sacrificial template method, and uniformly dispersing the discrete structural color hydrogel microspheres in an aqueous solution with certain viscosity to serve as an internal phase solution; selecting a hydrogel precursor solution which can be rapidly cured and has elasticity as an external phase solution; the internal phase solution and the external phase solution are simultaneously and respectively introduced into an internal phase capillary tube and an external phase capillary tube of a co-flow micro-fluidic chip, benefited from a laminar flow state in a micro-channel, the external phase solution can wrap the internal phase solution and is cured under ultraviolet, and the discrete structure color microsphere implanted fiber is obtained. The preparation method is simple and easy to operate, mass production can be realized, and the prepared microfiber has the advantages of repeatability, convenience in operation, reliable result and the like.

The invention relates to an in-glaze jellyfish drop Jianjian cup. Specifically, the invention provides the preparation process of the in-glaze jellyfish drop Jianzhan cup, and the process comprises the following steps: preparing a green body and glaze slip, applying the glaze slip to the green body, and roasting the glazed green body to obtain the in-glaze jellyfish drop Jianzhan cup. The jellyfish drops in the glaze prepared by the process disclosed by the invention are black brick bodies, golden edges and rich and graceful breath and charm, blue hair filaments are arranged below the edges, the hair filaments are fine, clear, dense, clear and uniform, the quality of the jellyfish drops is beautiful jade, the layering sense is strong, and the jellyfish drops (oil drops are similar to jellyfish) which are rich in sensitiveness are arranged in the glaze. The jellyfish drops are moist, the mimicry is fresh, the jellyfish has excellent texture, the glaze surface is free of plaques and pores, the texture is good, and therefore the jellyfish has graceful art and collection values.

The invention relates to the technical field of biomedicine, and provides a nanobody KY031 against jellyfish toxin, a preparation method and application. The nanobody is a VHH antibody and has the amino acid sequence shown in SEQ ID NO. 1. The present invention is analyzed by affinity The nanobody has good affinity. It has been proved by small animal experiments that the mice in the antibody protection group pre-injected with the nanobody of the present invention have no symptoms of poisoning after the injection of jellyfish toxin, and no toxin fatality has been observed for one month. The nanobody of the present invention has excellent anti-jellyfish toxin effect, has excellent preventive or therapeutic effect on jellyfish stings, and has broad clinical application prospects.