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Jellyfish mucin separation method and application thereof in repairing cosmetics

A separation method and mucin technology, applied in the direction of cosmetics, peptide preparation methods, cosmetic preparations, etc., can solve the problems of large consumption of organic solvents, crude sample purification, complex process, etc., to improve the purity and protect the gastrointestinal mucosa , Improve the effect of economic value

Inactive Publication Date: 2020-12-25
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This difference may be due to the fact that the patent did not further purify the crude sample, resulting in a large amount of collagen in the mucopolysaccharide
[0008] The process of patent 2013800318499 is complex and requires the consumption of a large amount of organic solvents
In addition, the entire production process consumes a large amount of electric energy to keep the material at a low temperature during the production process

Method used

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  • Jellyfish mucin separation method and application thereof in repairing cosmetics
  • Jellyfish mucin separation method and application thereof in repairing cosmetics
  • Jellyfish mucin separation method and application thereof in repairing cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Take several sheets of fresh white jellyfish (total weight about 10kg), rinse them with clean water, cut them from the top of the umbrella, dig out the middle glue layer, and get about 1.2kg of gel-like substance;

[0038] (2) Homogenize the jelly, put the homogenate into an ultrasonic extraction kettle, add 2L 0.5% trisodium citrate solution and 1.6g sodium dehydroacetate, set the ablation conditions as ultrasonic pulse power 2000W, heating temperature 45°C , the time is 2h;

[0039] (3) Add about 20 mL of 10% citric acid solution to the dissolved mucus, mix well, then add 1.6L of 95% ethanol, mix well, and let stand for 2 hours;

[0040] (4) Centrifuge at 5000g for 30min to collect about 4.5L of supernatant;

[0041] (5) Add 250mL of 10% citric acid to the supernatant, then add 2.5L of 95% ethanol, mix well, and let stand;

[0042] (6) Centrifuge at 5000g for 30min to collect the precipitate, which can be rinsed twice with 50% ethanol containing 0.5% citric acid...

Embodiment 2

[0045] An essence containing mucin, the formulation of which is shown in Table X.

[0046] Table 1 A formula of essence with skin barrier repairing effect

[0047]

[0048]

[0049] In order to prove the effectiveness of the present invention, the inventor carries out following test:

[0050] 1) GPC determination of molecular weight and purity

[0051] experimental method:

[0052]Take 25 mg of lyophilized mucin sample and dissolve it with 10 mL of mobile phase, filter through a 0.45 μm filter head and inject 25 μL into a high performance gel chromatograph. Dextran (580,000Da) was used as the reference substance, and Shimadzu GPC software was used to analyze the molecular weight and molecular weight distribution of the sample.

[0053] Instrument Conditions:

[0054] The chromatographic column is TSK-gel; column temperature: 40°C; mobile phase: 0.05mol / L sodium nitrate; flow rate: 0.6mL / min; Shimadzu RID-20 differential refractive index detector (40°C).

[0055] Exp...

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Abstract

The invention relates to a jellyfish mucin separation method and application thereof in repairing cosmetics. High-purity and high-viscosity mucin is separated from the mesoglea of a jellyfish by utilizing the difference of isoelectric points of the mucin and collagen and combining ethanol fractional precipitation; and clinical tests on human bodies show that the mucin can repair damaged skin barriers and reduce skin sensitivity. The method comprises the following steps of: rinsing a jellyfish, cutting the jellyfish open from the top end of an umbrella part, and digging out mesoglea jelly; homogenizing the jelly, and putting homogenate into an ultrasonic extraction kettle to be ablated to obtain ablated mucus; adjusting the pH value of the ablated mucus to 5-8, and adding ethanol to make the ethanol to reach 10-40%; performing primary centrifugation, and collecting supernatant of the primary centrifugation; adjusting the pH value of the supernatant of the primary centrifugation to 2-4,and adding ethanol to make the ethanol to reach 40-70%; performing secondary centrifugation, collecting precipitate, and rinsing the precipitate with acidic ethanol; and redissolving the precipitate with buffer solution, and performing freeze-drying to obtain the white cotton mucin.

Description

Technical field: [0001] The invention belongs to the technical field of development and utilization of marine active ingredients and the field of cosmetics development, and specifically relates to a separation method of jellyfish mucin and its application in repairing cosmetics. Background technique: [0002] Mucin, also known as mucin, is a kind of highly glycosylated polymer protein, mainly secreted by epithelial cells, with gel-like properties of mucosal barrier, and plays an important role in the immune defense of the respiratory tract and digestive tract . Mucin products are mainly derived from the secretions of mandibular glands or gastric mucosal mucus of cattle, pigs and sheep. Tokio Yamashita of Japan and others applied the mucin secreted by the bovine submandibular gland to cosmetics, and proved through experiments that it has moisturizing and wrinkle-removing effects. In recent years, domestic enterprises have also made some progress in extracting and isolating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/30A61K8/64A61Q19/00
CPCA61K8/64A61Q19/00C07K14/43595
Inventor 潘发伍林晓锋王希丽甘丽娜胡蓉青王文俊
Owner 嘉文丽(福建)化妆品有限公司
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