A kind of polypeptide extracted from the venom of fire jellyfish cnidaria and its application
A fire jellyfish sting and venom technology, applied in the field of peptides, to achieve the effect of fewer separation steps, rapid separation, and mild separation conditions
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Embodiment 1
[0042] A polypeptide molecule, the amino acid sequence of which is shown in SEQ ID No.1;
[0043] SEQ ID No. 1:
[0044]
[0045]
Embodiment 2
[0047] The extraction of the polypeptide of the present invention:
[0048] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 0°C in a volume ratio of 1:1. After stirring and standing still, take the precipitate and repeat it twice. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;
[0049] Step 2. Mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 5.5 according to the mass volume ratio of 1g: 40mL, and ultrasonically crush it for the second time. Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;
[0050] Step 3, take the venom protein obtained in step 2, dilute it 3 times with a buffer solution of disodium hydrogen phosphate-sodium dihydrogen ...
Embodiment 3
[0054] The extraction of the polypeptide of the present invention:
[0055] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 4°C at a volume ratio of 1:1.5. After stirring and standing still, take the precipitate and repeat it for 4 times. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;
[0056] Step 2, mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 6.5 according to the mass volume ratio of 1g: 60mL, and ultrasonically crush it for the second time Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;
[0057] Step 3, take the venom protein obtained in step 2, dilute it 5 times with the disodium hydrogen phosphate-sodium dihydrogen phosphate ...
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