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Jellyfish active protein as well as preparation method and application thereof

A technology of active protein and jellyfish, which is applied in the field of jellyfish active protein and its preparation, can solve the difficult problems of jellyfish collagen quality control, uncontrolled degradation of collagen, and uncontrollable, so as to promote adherent growth and improve skin moisturizing Sexuality, the effect of repairing the skin barrier

Active Publication Date: 2021-11-02
MELLGEN SHENZHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the commonly used separation procedures, there are also some disadvantages that cannot be ignored: acid treatment will cause partial hydrolysis during the extraction process, which is largely uncontrollable, and the collagen produced is collagen that has undergone hydrolysis / degradation to varying degrees A mixture of protein peptides and intact collagen; the extracted collagen also degrades uncontrolled during pepsin treatment and extraction because the tissue itself cannot be completely removed by washing the tissue prior to extraction to remove its own enzymes
Therefore, it is difficult to control the quality of jellyfish collagen in aqueous solution
Conventional collagen can only be used in freeze-dried form and stored for a long time

Method used

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  • Jellyfish active protein as well as preparation method and application thereof
  • Jellyfish active protein as well as preparation method and application thereof
  • Jellyfish active protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 urea pepsin method

[0043] 1. Soak the sample in urea solution

[0044] Jellyfish skin (Rhopilema esculentum, jellyfish genus) was purchased from the aquatic market. Cut 10kg of pickled jellyfish into small pieces, wash them with tap water, and then soak them in pure water for several hours to remove salt. Add 1 gram of delipase (purchased from Shanghai Sangong), soak at 25° C. for 4 hours, and wash off the delipase. Divide the tissue block into 5 parts, 2kg each, add 4 liters of urea solution with a concentration of 1mol / L, 2mol / L, 3mol / L, 4mol / L, 5mol / L respectively, keep stirring at room temperature and low speed for 2 hours, in order to ensure the subsequent enzymatic To promote the reaction, urea needs to be removed, and at this time filter the tissue block with a filter cloth. The results showed that the tissue blocks melted to varying degrees after soaking in urea above 3mol / L, and the tissue loss was more when washing with urea. After soaking i...

Embodiment 2

[0049] Embodiment 2: pepsin method

[0050] Cut 10kg of pickled jellyfish into small pieces, wash them with tap water several times, and then soak them in pure water for several hours until the salinity is lower than 0.01. Add 1 gram of delipase, keep soaking at 25°C for 4 hours, and wash off the delipase. The following steps are carried out below 20°C. Add 0.5M citric acid to homogenize the material, then transfer to a stirring tank, add 10 mg / g pepsin (purchased from Shanghai Sangon Biology) solution for enzymatic digestion, and keep stirring at 16°C for 12 hours. After digestion, centrifuge at 17,000g for 30 minutes to separate undigested tissue pieces, neutralize the separated supernatant to pH 7.0 with sodium hydroxide, and then centrifuge at 17,000g for 20 minutes to collect the collagen gel precipitate. The collagen was then dissolved with 0.05% citric acid, then the supernatant was adjusted to neutral, and the gel precipitate was collected by centrifugation, and the ...

Embodiment 3

[0051] Embodiment 3: acid extraction process

[0052] Cut 10kg of pickled jellyfish into small pieces, wash them with tap water several times, and then soak them in pure water for several hours until the salinity is lower than 0.01. Add 1 gram of delipase, keep soaking at 25°C for 4 hours, and wash off the delipase. The following steps are carried out below 20°C. Add 0.5M citric acid to homogenize the material, transfer it to a stirring tank, and keep stirring at a slow speed for 12 hours at 16°C. After digestion, centrifuge at 17,000g for 30 minutes to separate undigested tissue pieces, neutralize the separated supernatant to pH 7.0 with sodium hydroxide, and then centrifuge at 17,000g for 20 minutes to collect the collagen gel precipitate. The collagen was then dissolved with 0.05% citric acid, then the supernatant was adjusted to neutral, and the gel precipitate was collected by centrifugation, and the steps from dissolution to neutralization were repeated twice. Swell t...

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Abstract

The invention discloses a preparation method of jellyfish active protein. The preparation method comprises the following steps: 1, preparation of collagen or collagen sponge: extracting collagen or collagen sponge from jellyfish; and 2, dissolving collagen or collagen sponge in a high-concentration urea solution to obtain the collagen sponge. The invention further discloses jellyfish active protein and application thereof. According to the invention, after the collagen sponge is extracted, high-concentration urea is added for denaturation, so that insoluble tissues or cell debris and lipid substances which are co-precipitated can be separated.

Description

technical field [0001] The invention relates to the technical field of jellyfish active protein preparation, in particular to a jellyfish active protein and its preparation method and application. Background technique [0002] Collagen can be extracted from a variety of organisms. Preferred sources of collagen for tissue engineering applications are bovine skin, tendon and porcine skin. However, collagen of bovine origin carries the risk of being infected by diseases such as bovine spongiform encephalopathy (commonly known as mad cow disease). Furthermore, mammalian collagens, especially those of porcine origin, are increasingly rejected for religious reasons. Marine organisms are a natural source of collagen replacement and are supposedly safer compared to mammals. Another attractive marine source for extracting collagen is jellyfish. The increase in global jellyfish populations is causing major problems in the ecological environment, and its potential application in ti...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/30C07K1/14A61K8/64A61Q19/00A61K38/39A61P17/00
CPCC07K14/78C12P21/06A61K8/64A61Q19/00A61P17/00A61K38/00
Inventor 阮仁全王辉徐蕾丁卫平
Owner MELLGEN SHENZHEN BIOTECHNOLOGY CO LTD
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