38results about How to "The detection signal is obvious" patented technology

The invention discloses a mercury ion detection reagent and a detection method. The reagent comprises nano sol, nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral signal and carrying out data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface nucleic acid adaptors are changed, i.e. nano particle aggregation in the silver sol and surface enhanced Roman scattering signals of signal molecules are enhanced. In the invention, the SERS (Surface Enhanced Raman Scattering) technology is adopted, the detection limit to the Hg<2+> is at the nmol / L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and high selectivity and is suitable for the quick detection of trace Hg<2+> in environmental water.

The invention provides a fluorescent probe for distinguishing detection of Cys / Hcy and GSH and a preparation method and application of the fluorescent probe. The English name of the fluorescent probeis 10-(diethylamino)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)oxy)-5,6-dihydrobenzo[c]xanthen-12-ium, and the fluorescent probe is called as NBD-O-1. The invention provides a method for detecting theCys / Hcy and GSH by the fluorescent probe NBD-O-1. In a PBS solution with the pH of 7.4 and v / v of 2 / 8 and containing 20% CH3CN, the qualitative distinguishing detection of the Cys / Hcy and GSH is achieved through an ultraviolet and fluorescent spectrometer without the interference of common amino acids. More importantly, the probe can be applied to the detection of the Cys / Hcy and GSH in animals and plants, the detection process is convenient, sensitive and rapid, and a detection result is accurate.

The invention discloses a Fe-Ga material based magnetostriction liquid-level sensor. The liquid-level sensor is composed of a sensor head, a measuring rod and a floater, the sensor head is connected with the measuring rod in a threaded manner, and the moving floater sleeves the lower portion of the measuring rod. The sensor head is of a cylindrical structure, and a hardware circuit system, a detecting device and a front damping device are arranged in the sensor head. A Waveguide wire is made of Fe-Ga materials and arranged in the measuring rod. The front end of the waveguide wire enters the sensor head through a threaded hole and sequentially penetrates a coil frame in the detecting device and the center of the front damping device till to the hardware circuit system. The tail end of the waveguide wire penetrates the center of a rear damping device to be connected with a return lead, the return lead is connected to a hardware circuit to form a closed loop. The Fe-Ga alloy is used as materials of the waveguide wire of the magnetostriction liquid-level sensor and can be applied to wide-range liquid-level sensors, and the Fe-Ga material based magnetostriction liquid-level sensor can stably work in the severe environment.

The invention provides combination of primers and a CRISPR sequence for detecting novel coronavirus gene comprising two groups of primer pairs for RPA isothermal amplification for respectively detecting virus ORF1ab genes and N genes, and two crRNA sequences capable of respectively detecting RPA amplification products. The ORF1ab gene and the N gene can be detected at the same time through systemdetection, operation is easy and convenient, and each detection sample only needs to be detected through a single tube; the whole reaction process is carried out at the temperature of about 37 DEG C,a fine temperature control element and complex temperature change which are required by PCR amplification are not required, and the combination is suitable for a basic apartment to detect by using relatively cheap instant detection equipment; and the sensitivity reaches 10 copies / uL. Compared with a PCR technology, the advantages are that the speed is higher, an obvious detection signal can be obtained within 30 min, and whether the 2019-nCoV novel coronavirus nucleic acid result of a sample is positive or not can be rapidly judged.

The invention provides a fluorescent probe for distinguishing and detecting mercaptan and monitoring Cys / GSH metabolism as well as a preparation method and application of the fluorescent probe. The Chinese name of fluorescent probe is 10-(diethylamino)-3-((7-(diethylamino)-3-formyl-2-oxo-2H-chromen-4-yl) oxy)-5, 6-dihydrobenzo [c] xanthene-12-onium perchlorate, the English name is 10-(diethylamino)-3-((7-(diethylamino)-3-formyl-2-oxo-2H-chromen-4-yl)oxy)-5,6-dihydrobenzo[c]xanthen-12- ium perchlorate,, which is named as CM-O-AC. The invention provides a red fluorescent probe with multiple binding sites, good water solubility and large Stokes shift. The probe displays three different emission channels for distinguishing and detecting Cys / Hcy and GSH, and is successfully applied to HeLa celland living body imaging. In addition, the probe can realize recognition of the Cys / GSH metabolite SO2 at a cellular level, and realizes reversibility of a fluorescence signal under the action of H2O2. The probe can be used as a visual biological metabolism tool to be applied to physiological and pathological processes. The detection process is simple, sensitive and rapid, and the detection resultis accurate.

The invention relates to a magnetostriction micro displacement meter, which is characterized in that the displacement meter comprises a displacement meter end part, a displacement meter base, a measuring rod, a waveguide wire, a linear guide rail and a movable terminal, the displacement meter end part is of a cavity structure, the inner wall of the cavity structure is provided with a hardware circuit system, and a cavity body of the cavity structure is internally provided with a front damping device, a detection device and a fixing device; the bottom part at the right side of the displacement meter end part is connected with the displacement meter base; the center at the right side of the displacement meter end part is provided with a threaded hole, and the left end of the measuring rod is in threaded connection with the threaded hole of the displacement meter end part; the movable terminal is arranged on the measuring rod in a sleeved mode, the linear guide rail is fixed on the displacement meter base along the length direction, the linear guide rail is provided with a sliding block, the upper end of the sliding block is connected with the lower end of the movable terminal, and the sliding block is connected with an external mechanical device; and magnets in the movable terminal adopt a halbach magnet array.

The invention provides a benzothiazole derivative and a synthesis method and application thereof. The Chinese name of the derivative is called as (E)-2-(3-(benzo[d]thiazole-2-yl)-2-hydroxy-5-methylstyryl)-3-methylbenzo[d]thiazole-3-iodide, and the structural formula of the derivative is shown in the specification. The English name of the compound is (E)-2-(3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methlyryl)-3-methlylbenzo[d]thiazol-3-iumiodide, and the structural formula of the compound is shown in the specification. The reagent is named as NSSN, and can realize dual-mode detection of hypochlorousacid. According to the detection method, in a buffer solution of PBS / CH3CN (1: 1, v / v, pH=7.4), the content of hypochlorous acid is detected through a fluorescence spectrophotometer ratio type and aturn-on type. The detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention provides an indole salt-coumarin derivative, and a synthesis method and an application thereof. The English name of the derivative is (E)-1-(2-carboxyethyl)-2-(2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)vinyl)-3,3-dimethyl-3H-indol-1-ium, and the reagent is named as Mito-CI. The reagent can be used for efficiently and reversibly detecting sulfur dioxide. A detection method is characterized in that sulfur dioxide is detected through a fluorospectro photometer in a pure PBS buffer solution with the pH value of 7.4. The detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention discloses a magnetostrictive displacement sensor suitable for a large temperature range / high temperature environment. The sensor comprises a pulse generating circuit, a constant elasticdevice, a detecting coil, a control sampling module, a magnetostrictive waveguide wire, a casing, a thermocouple, a waveguide wire insulating sleeve and a material level mark. A left side of the casing is a component bin, a right side of the casing is a hollow pipe, and the component bin and the hollow pine communicate with each other and have the same center axis. The magnetostrictive waveguide wire is Fe-Ga or Fe-Co material. The waveguide wire is fixed in the sensor casing. The detection coil sleeves a left side of the waveguide wire. The material level mark sleeves a right end of the casing. A magnetic material of the sensor obtained by the invention has a higher Curie temperature, and the novel displacement sensor can be applied to a high-temperature environment below 500 DEG C.

The invention provides a method for detecting lime substances in flour with a near-infrared micro-imaging technology. The method comprises the steps of: collecting a near-infrared micro-image of a flour sample; according to the near infrared spectrum characteristics of lime substances and pure flour, subjecting the near-infrared micro-image of the flour sample to an imaging analysis. Without any complex pretreatment in the whole process, the detection of the invention has simple, rapid and pollution-free steps. Compared with traditional methods, the method provided in the invention has more obvious additive detection signals, thus being more conducive to the detection of very small amounts of additives. As near-infrared lights are stronger than mid-infrared lights in terms of penetrability in substances, so they can adapt to rough surfaces. Besides, near-infrared lights not only can collect the information of the sample surface, but also can go deep into the sample to collect information. Therefore, the near-infrared micro-imaging technology is very suitable for detection of lime substances in flour, thus providing a new method for rapid quality safety screening of market flour.

The invention discloses a method for detecting miRNA-21 by a blood glucose meter based on dnazyme and sucrase. The method comprises the following steps: coupling a substrate chain and a dnazyme chain silenced by a sway chain on a magnetic bead, allowing target miRNA to be hybridized and combined with the sway chain after the target miRNA appears, and separating from the dnazyme chain so as to activate the dnazyme to cut the substrate chain; and allowing the released sucrase to hydrolyze sucrose to glucose, and acquiring the output signal by the detection of a blood glucose meter. According to the method, the detection signal of the miRNA is subjected to double enzyme amplification by introducing the dnazyme and the sucrase, so that the detection sensitivity is improved, a linear relation exists in the range of 100-1000fM, the detection limit is 68.08fM, and the method has the advantages of simplicity, convenience, rapidness, sensitivity, high efficiency, low cost and the like.

The invention discloses a miRNA-21 detection method. According to the method, a substrate chain-sucrase complex is coupled on each magnetic bead, when a target miRNA appears, the target miRNA hybridizes with the substrate chains, double-strand specific nuclease can selectively cleave the DNA in DNA / RNA double strands and keep the activity of RNA, and the released miRNA is subjected to the next round of hybridizing cycle; and finally, the sucrase in supernatant hydrolyzes sucrose into glucose, and an output signal is obtained through the detection using a glucometer. The invention improves detection sensitivity by introducing the double-strand specific nuclease and the sucrase to carry out double-enzyme amplification on the detection signals of the miRNA, a linear relation is realized at 10-200 pM, a limit of detection is 1.8 pM, and the invention has the advantages that the detection method is simple and convenient, fast, sensitive, highly-efficient, etc.

The invention provides a fluorescent probe for specifically responding to hypochlorous acid based on BODIPY dye and preparation and application thereof. The structural formula of the probe is shown in the specification. According to the probe, the BODIPY dye is selected as a fluorophore, and an N, N-dimethylamino thioformate group is selected as a recognition group. The detection mechanism is that the N, N-dimethylamino thioformate group is oxidized and removed by hypochlorous acid to cause electron transfer, and phenate is further subjected to a self-elimination reaction to release the fluorophore so as to cause the change of a fluorescence signal. The probe is used for detecting ClO <-> and is not interfered by other active nitrogen and active oxygen substances, the operation process is simple, convenient, rapid and sensitive, and the detection limit is 1.8 nM. More importantly, the probe can detect ClO <-> in cells and living bodies, and has a good application prospect in the field of biological monitoring.

The invention provides a fluorescent probe for selectively responding to H2S based on BODIPY fluorescent dye targeting lysosome, and preparation and application of the probe. The probe selects BODIPY dye as a fluorophore and 2, 4-dinitrobenzenesulfonyl as a recognition group. A sensing mechanism is based on H2S to induce cracking of 2, 4-dinitrobenzenesulfonyl in the probe, so that a photoinduced electron transfer (PET) process is inhibited. The probe detects H2S through ultraviolet and fluorescence spectrometers and is not interfered by other amino acids, active sulfides, representative anions and the like, the detection process is simple, convenient, rapid and sensitive, and the detection limit is 4.75 nM. More importantly, the probe can target lysosome and detect H2S in cells at the same time, and has a good application prospect in the field of biological monitoring.

The invention provides a BODIPY derivative BDO-N3 and a synthetic method and the usage thereof. The English name of the derivative is 1-(4-azidobenzy1)-4-(5,5-difluoro-1,3,7,9-tetramethy1-5H-4lambda<4>,5lambda<4>-dipyrrolo[1,2-c:2',1'-f][1,3,2]diaz aborinin-10-y1)pyridin-1-ium. The invention further provides a method for detecting hydrogen sulfide through specificity of being sensitive to viscosity, and in DMSO (dimethyl sulfoxide), the content of hydrogen sulfide is detected through a fluorospectrophotometer. In addition, in a methanol and glycerol system, with the increase of viscosity, BDP-N3 is used for detecting viscosity change through the fluorospectrophotometer. The detecting is simple in process, sensitive and quick, and the detecting results are accurate.

The invention provides a fluorescent probe based on phenyl selenide group specific response Cys, and a preparation method and application thereof. According to the probe, BODIPY dye is selected as a fluorophore, and diphenyl selenide is selected as a recognition group. The detection mechanism is that Cys and diphenyl selenide are subjected to nucleophilic substitution-rearrangement reaction to cause fluorescence signal change. The probe detects Cys through ultraviolet and fluorescence spectrometers and is not interfered by other amino acids and active oxygen, the detection process is simple, convenient, rapid and sensitive, and the detection limit is 33 nM. More importantly, the probe can be used for detecting Cys in cells and has a good application prospect in the field of biological monitoring.

The invention discloses a magnetostrictive displacement sensor suitable for a large temperature range / high temperature environment. The sensor comprises a pulse generating circuit, a constant elasticdevice, a detecting coil, a control sampling module, a magnetostrictive waveguide wire, a casing, a thermocouple, a waveguide wire insulating sleeve and a material level mark. A left side of the casing is a component bin, a right side of the casing is a hollow pipe, and the component bin and the hollow pine communicate with each other and have the same center axis. The magnetostrictive waveguide wire is Fe-Ga or Fe-Co material. The waveguide wire is fixed in the sensor casing. The detection coil sleeves a left side of the waveguide wire. The material level mark sleeves a right end of the casing. A magnetic material of the sensor obtained by the invention has a higher Curie temperature, and the novel displacement sensor can be applied to a high-temperature environment below 500 DEG C.

The invention provides a method for detecting lime substances in flour with a near-infrared micro-imaging technology. The method comprises the steps of: collecting a near-infrared micro-image of a flour sample; according to the near infrared spectrum characteristics of lime substances and pure flour, subjecting the near-infrared micro-image of the flour sample to an imaging analysis. Without any complex pretreatment in the whole process, the detection of the invention has simple, rapid and pollution-free steps. Compared with traditional methods, the method provided in the invention has more obvious additive detection signals, thus being more conducive to the detection of very small amounts of additives. As near-infrared lights are stronger than mid-infrared lights in terms of penetrability in substances, so they can adapt to rough surfaces. Besides, near-infrared lights not only can collect the information of the sample surface, but also can go deep into the sample to collect information. Therefore, the near-infrared micro-imaging technology is very suitable for detection of lime substances in flour, thus providing a new method for rapid quality safety screening of market flour.

The invention provides a triphenylamine-benzopyran onium salt derivative NIR-BT-P as well as a synthesis method and application thereof. The derivative NIR-BT-P has a Chinese name of (E)-6-(diethylamino)-4-(4 (diphenylamino) benzylidene)-1, 2, 3, 4 tetraoxypyrimidine, and the derivative NIR-BT-P has a structural formula shown in the description. The invention relates to a preparation method of a diethylene glycol dimer, which is characterized in that the diethylene glycol dimer has an English name of (E)-6-(diethylene glycol dimer)-4-(4 (diethylene glycol dimer)-1, 2, 3, 4 tetraethylene glycol dimer, and the English name of the diethylene glycol dimer is (E)-6-(diethylene glycol dimer)-4 (4)-1, 2, 3, 4 tetraethylene glycol dimer. The derivative NIR-BT-P is used as a fluorescent probe, and in different organic solvents, detection of polarity by different fluorescence intensity signals is realized through a fluorospectro photometer. According to the method, the difference of polarities in cancer cells and normal cells in a living body is utilized, so that the near-infrared fluorescence imaging is used for distinguishing and detecting tumor tissues and normal tissues in the living body.

The invention discloses a detector for the coagulation function of whole blood. The detector comprises a detecting vessel, a rotating device, a light wave generator, a light wave receiver, a timer and a shell, wherein the detecting vessel is used for placing the whole blood; the rotating device, the light wave generator, the light wave receiver, the timer and the shell are connected with the detecting vessel; the rotating device is used for driving the detecting vessel to rotate around the self central axis; the light wave generator and the light wave receiver are oppositely arranged on the two sides of the detecting vessel; when the whole blood inside the detecting vessel is not coagulated, the light wave receiver always receives the quite strong light emitted by the light wave generator continuously, cannot or can only receive the weak light signal emitted by the light wave generator in the rotation process of the detecting vessel; and the timer is used for recording the time that the light wave receiver, into which a coagulation reagent is added, cannot receive the light emitted by the light wave generator for the first time or cannot stably receive the light emitted by the light wave generator, or the time that the light wave receiver receives the quite strong light signal for the first time. The invention also discloses a method for detecting the coagulation function of the whole blood.

The invention discloses a mercury ion detection reagent and a detection method. The reagent comprises nano sol, nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral signal and carrying out data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface nucleic acid adaptors are changed, i.e. nano particle aggregation in the silver sol and surface enhanced Roman scattering signals of signal molecules are enhanced. In the invention, the SERS (Surface Enhanced Raman Scattering) technology is adopted, the detection limit to the Hg<2+> is at the nmol / L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and high selectivity and is suitable for the quick detection of trace Hg<2+> in environmental water.

The invention relates to an optical device, in particular to an optical polarizer and a method for adjusting its asymmetric transmission characteristics. The optical polarizer includes a base layer and a planar structure, and the planar structure is composed of a plurality of nano-period units with the same structure Rectangular periodic arrays are connected, and each periodic unit contains a structural unit. The structural unit is composed of a rectangular hole and a semicircular hole. The arc of the semicircular hole is separated from or tangent to the long side of the rectangular hole; The diameter of the circular hole is parallel to the long side of the rectangular hole; each nano-period unit is made of noble metal material, and the semicircular hole is completely filled with metal Mg. The structure of the optical polarizer of the present invention can achieve the purpose of adjusting its asymmetric transmission characteristics through the transformation of the properties of the metal filled in the semicircular hole. The adjustment method is simple and convenient, and provides a new idea for the design of the optical polarizer.

The invention relates to an optical component, in particular to an optical polarizer and an adjustment method of the asymmetric transmission characteristic thereof. The optical polarizer comprises a substrate layer and a plane structure, wherein the plane structure is formed by connecting a plurality of structurally identical nanometer periodic units in a rectangular periodic array; each periodicunit comprises a structural unit; the structural unit is formed by combining a rectangular hole and a semicircular hole; the circular arc of the semicircular hole is away from or tangent to the long side of the rectangular hole; the diameter of the semicircular hole is parallel to the long side of the rectangular hole; each nanometer periodic unit is made of a precious metal material; the semicircular hole is completely filled with metal Mg. The optical polarizer structure can achieve nature transformation by filling the semicircular hole with the metal, so that the purpose of adjusting the asymmetric transmission characteristic thereof is achieved; the adjustment method is simple and convenient; a new idea is provided for design of the optical polarizer.

The invention relates to a magnetostrictive micro-displacement meter, which is characterized in that the displacement meter includes a displacement meter end, a displacement meter base, a measuring rod, a waveguide wire, a linear guide rail and a movable terminal, and the displacement meter end is a cavity structure , a hardware circuit system is installed on the inner wall of the cavity structure, and a front damping device, a detection device and a fixing device are arranged in the cavity of the cavity structure; the right bottom of the end of the displacement meter is connected with the base of the displacement meter; The center of the right side of the end is provided with a threaded hole, and the left end of the measuring rod is threadedly connected with the threaded hole at the end of the displacement meter; the movable terminal is set on the measuring rod, and the linear guide rail is fixed on the base of the displacement meter along the length direction. A slider is installed on the top, the upper end of the slider is connected with the lower end of the movable terminal, and the slider is connected with an external mechanical device; the magnet inside the movable terminal adopts a "halbach" magnet array.

The invention provides a BODIPY-based fluorescent dye targeting lysosomes selectively responding to H 2 The fluorescent probe, preparation and application of S, the probe uses BODIPY dye as the fluorophore, and 2,4-dinitrobenzenesulfonyl as the recognition group. The sensing mechanism is based on the H 2 S induces the cleavage of the 2,4‑dinitrobenzenesulfonyl group in the probe, thereby inhibiting the process of photoinduced electron transfer (PET), resulting in the restoration of probe fluorescence. The above probes were detected by UV and fluorescence spectrometers for H 2 S is free from interference from other amino acids, active sulfides, and representative anions. The detection process is simple, rapid, and sensitive, with a detection limit of 4.75 nM. More importantly, the probe can target lysosomes and simultaneously detect H in cells 2 S, has a good application prospect in the field of biological monitoring.

The invention provides an isoxazole derivative as well as a synthesis method and application thereof to detection of hydrogen sulfide. The Chinese name of the isoxazole derivative is (E)-5-(2-(5,5-difluoro-10-(4-methoxyphenyl)-1,3,7,9tetramethyl-5hydrodipyrrolo[1,3,2]diazaboro-2-yl)vinyl)-3-methyl-4-nitroisoxazole. The invention also provides a method for rapidly detecting the hydrogen sulfide; based on the isoxazole derivative, the method is used for quantitatively detecting the content of the hydrogen sulfide in a PBS (Phosphate Buffer Solution) (with the pH (Potential of Hydrogen) of 7.4) through a fluorescence spectrometer. By adopting the method, the hydrogen sulfide is rapidly and conveniently detected.