30results about How to "Realize differentiated detection" patented technology

The invention discloses an electromagnetic ultrasonic longitudinal guided wave and magnetic leakage detection compounded nondestructive detection method. On the original basis of magnetic leakage, an electromagnetic ultrasonic (EMAT) exciting coil and an electromagnetic ultrasonic (EMAT) receiving coil are added. When the detection process is normal, only magnetic leakage detection is performed. When a defect is found by the magnetic leakage method, the electromagnetic ultrasonic (EMAT) exciting coil sends an ultrasonic longitudinal guided wave on the inner surface of a steel plate to be detected. Through analyzing the ultrasonic longitudinal guided signal received by the electromagnetic ultrasonic (EMAT) receiving coil, it can be determined whether a defect exists in the inner surface of the steel plate to be detected, thus determination of defects in the inner / outer surfaces of the steel plate to be detected is realized. The electromagnetic ultrasonic longitudinal guided wave and magnetic leakage detection compounded detection method effectively overcomes the shortages of low accurate rate of determination on defects and low detection speed in the prior art, and has the advantages of simple structure, little information processing amount and non-contact measurement.

The invention discloses a novel closed type nucleic acid visual detecting method for coupling a nucleic acid amplification reaction, a nucleic acid intrusive reaction and a nano-particle chromogenic reaction. The detection method comprises the following steps: 1) target nucleic acid amplification and signal conversion phase: further amplifying the target nucleotide sequence, and converting the detection of the target nucleic acid to the detection of a hairpin probe fragment; 2) nanoprobe hybridization phase: after the amplification reaction, under the corresponding conditions, generating a gathering or dispersing phenomenon by the special nanoprobe, and utilizing the phenomenon to realize distinguishing detection to the target nucleic acid. With adoption of the detection method disclosed by the invention, the nucleic acid target sequence can be detected; furthermore, the nucleic acid intrusive reaction is high in specificity, and the nucleic acid sequence with single different basic groups near the intrusive loci can be distinguished and detected, so that the method not only can be used for detecting a template, but also can be used for single basic group distinguished SNP genotyping and gene mutation.

The invention discloses a method for differentiating multiple types of metal ions through single indicator. The method comprises the following steps of 1, mixing metal ion indicator with surfactant, or mixing the metal ion indicator with a NaOH aqueous solution, so that the single indicator is obtained; 2, mixing the single indicator with different metal ion aqueous solutions separately, collecting images obtained before and after mixing, and extracting color change data of the images obtained before and after mixing; 3, mixing the metal ion aqueous solution to be detected with the single indicator, collecting color change of the images before and after the metal ion aqueous solution to be detected is mixed with the single indicator, and comparing the color change with the color change data obtained in step 2, so that the categories of the metal ions in the metal ion aqueous solution to be detected are obtained. By means of the method for differentiating multiple types of metal ions through the single indicator, multiple types of metal ions in the aqueous solution can be rapidly detected.

The invention discloses a fluorescent probe with multiple active sites as shown in structural formula I. The fluorescent signal intensity of the probe for Cys is 80 times that of Hcy, 30 times that of GSH, and 30 times that of SO 2 260 times higher, can detect Cys with high selectivity, and distinguish Hcy, GSH and SO at the same time 2 Interference of Hcy, GSH and SO 2 The detection limits of Cys are 0.21μM, 0.35μM, 0.15μM, respectively, and the detection limit of Cys is as low as 13nM; the cytotoxicity of the probe is weak, through the cell imaging test, it can be clearly observed that the probe is effective for Cys, Hcy, GSH and SO 2 Fluorescent response changes for biothiols and SO in vitro and in vivo 2 detection.

The invention provides a fluorescent probe for distinguishing and detecting Cys / Hcy and GSH, its preparation method and application. The Chinese name of the fluorescent probe is 10‑(diethylamino)‑3‑((7‑nitrobenzo[c][1,2,5]oxadiazol‑4‑yl)oxy)‑ 5,6‑Dihydrobenzo[c]xanthene12ium, named NBD‑O‑1. The invention provides a method for the detection of Cys / Hcy and GSH by fluorescent probe NBD-O-1, which is prepared in PBS (pH 7.4, containing 20% ​​CH 3 CN, v / v) solution, the qualitative distinction of Cys / Hcy and GSH by ultraviolet and fluorescence spectrometers is not interfered by common amino acids. More importantly, the probe can be applied to the detection of Cys / Hcy and GSH in animals and plants, and the detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention belongs to the field of molecular biology and relates to cascade intrusion signal amplification reaction combined nanogold-oligonucleotide probe visual detection. The cascade intrusion signal amplification reaction combined nanogold-oligonucleotide probe visual nucleic acid detection method provided by the invention comprises the following steps of: 1) first-step intrusion signal amplification reaction, during which accumulation of flap fragments is formed; 2) flap fragment circulation phase, during which complementary hybridization of the flap fragments and hairpin probes is performed to form a flap fragment-hairpin probe special structure, and the hairpin probes are cut after the structure is recognized by enzymes; and 3) nanogold-oligonucleotide probe detection phase, during which results of the cascade intrusion signal amplification reaction are visually detected by the utilization of two different oligonucleotide modified nanogold probes. By the utilization of the detection method provided by the invention, visual detection of a nucleic acid target sequence can be carried out. In addition, as the intrusion signal amplification reaction has high specificity, differentiated detection of the nucleic acid sequences with single-base differences near intrusion sites can be realized.

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is a carbon quantum dot co-doped with nitrogen and phosphorus, and the carbon source of the fluorescent carbon quantum is naphthalene dicarboxylic acid. Fluorescent carbon quantum dots have a high fluorescence quantum yield. After the microorganisms lose their activity, they can enter the microorganisms to specifically mark them, effectively distinguishing the life and death of microorganisms. The invention discloses a preparation method of the above-mentioned fluorescent carbon quantum dot, the raw material is easy to obtain, the preparation method is tested, and it is suitable for preparing the fluorescent carbon quantum dot with high quantum yield. The invention discloses the use of the above-mentioned fluorescent carbon quantum dots in microbial activity detection and biomedical imaging. The fluorescent carbon quantum dots can effectively distinguish the life and death of microorganisms, and realize efficient detection of microbial activity; meanwhile, the fluorescent carbon quantum dots have low cytotoxicity, It is suitable for biomedical imaging detection in vitro or in vivo.

The invention discloses a method for detecting nucleic acid signal amplification of ligation nucleic acid intrusive reaction and cutting endonuclease reaction, which belongs to the field of molecular biology. The method comprises the following steps: 1) during nucleic acid intrusive signal amplification, accumulating flap fragments; 2) during flap fragment connection, connecting the flap fragments with another oligonucleotide on a molecular beacon to form a cutting endonuclease site; 3) during cutting endonuclease signal amplification, only cutting a molecular beacon chain so that the fluorescent group of the molecular beacon is separated from a quenching group, and a new integrated molecular beacon is hybridized with a flap fragment connected with the molecular beacon, thus amplifying a target nucleic acid signal; and 4) inspecting the target nucleic acid signals. The method is capable of inspecting nucleic acid target sequences and respectively inspecting nucleic acid sequences with single base difference near intrusive sites.

The invention provides a coumarin derivative CTT and its synthesis method and application. The Chinese name of the derivative CTT is: ethyl (E)-2-cyano-3-(7-(diethylamino)-2-oxo-4-(p-tolylthio)-2H-color Alken-3-yl) ethyl acrylate; English name is ethyl-2-cyano-3-(7-(diethylamino)-2-oxo-4-(p-tolylthio)-2H-chromen-3-yl)acrylate. The derivative CTT was used as a fluorescent probe in DMSO:PBS=1:4(v / v) solution, and the differential detection of three biological thiols with different fluorescent signals was realized by a fluorescence spectrophotometer. This method realizes multicolor fluorescence imaging for the differential detection of thiols in vivo.

The invention provides a coumarin derivative DOCOPA as well as a preparation method and an application thereof. The English name of DOCOPA is (E)-4-(3-(7-(diethylamino)-2-oxo-2Hchromen-3-yl)-3-oxoprop-1-en-1-yl) phenylacrylate). The preparation method comprises the steps that 3-acetyl-7-diethylaminocoumarin and p-hydroxybenzaldehyde are dissolved in acetonitrile firstly, a catalytic amount of piperidine is added, and reflux is performed until a reaction is performed sufficiently; an intermediate compound is further obtained; the intermediate compound and triethylamine are dissolved in dichloromethane, acryloyl chloride is added dropwise gradually to an ice bath, room-temperature stirring is performed until a reaction is performed sufficiently, a product is subjected to column chromatography separation after reduced-pressure evaporation, and the DOCOPA is obtained. The DOCOPA is applied to distinguishing and detection of cysteine and homocysteine in a pH7.8 system and imaging of the cysteine and the homocysteine in cells. The DOCOPA shows high sensitivity and selectivity for the cysteine and the homocysteine.

The invention discloses a perylene diimide bromine substitute and a method and application for preparing a gas-phase sensing film of a Bacillus anthracis marker based on the same, belonging to the technical field of organic semiconductor materials. The sensor film of the present invention mainly includes organic active assembly materials, and the organic active layer materials are perylene diimide bromides PBI-1Br, PBI-2Br, PBI-3Br and PBI-4Br, which are loaded on the On glass, silica gel or porous anodized aluminum substrate, and the coating concentration is 0.1 ~ 0.5uL / cm 2 . The picolinic acid sensor of the invention is convenient to prepare, simple to operate, and has obvious effect of distinguishing and detecting pyridine and its derivatives. In addition, the pyridine and its derivative sensor of the present invention has high stability and fast response time.

The invention discloses an electromagnetic phase amplitude analysis method for detecting oil abrasive particles and a detection device thereof, which is used for distinguishing and detecting ferromagnetic and non-ferromagnetic particles of oil metal particles (2) in a hydraulic system pipeline (1), and a retrieval device (3) comprising an externally penetrating electromagnetic detection device (31), characterized in that the retrieval device (3) also includes a wear particle concentration detection device (32) for detecting the concentration of metal particles in the oil, wherein the electromagnetic detection device (31) The abrasive particle concentration detection device (32) is arranged on the periphery of the same position as the detection hydraulic system pipeline (1). Obtain the relationship curve between signal phase and ferromagnetic particle content (percentage) through simulation or experiment, and project the actual signal phase onto the curve to obtain the ferromagnetic particle content, so as to realize the analysis of ferromagnetic materials and non-ferromagnetic materials of oil abrasive particles Differential detection of the content of each component.

The invention discloses an aldehyde group-containing metal iridium complex and an application thereof in detection and distinction of homocysteine and cysteine; the metal iridium complex has the structural formula defined in the specification, wherein R is H, phenyl or C1-C12 alkyl. The aldehyde group in the obtained metal iridium complex can undergo a reaction with homocysteine to generate thiazan, and carboxyl (COOH) on the generated thiazan can form hydrogen bonds with amino (NH2) in the metal iridium complex, so that the complex can give out light to generate a great blue shift and makes light increased, but cysteine does not have the phenomenon; therefore, the metal iridium complex can be made into a fluorescence probe for detection and distinction of homocysteine and cysteine, and has important significance on biological analysis and detection.

The invention provides a high-sensitivity hydraulic oil liquid detection device and a manufacturing method thereof. The high-sensitivity hydraulic oil liquid detection device is used for distinguishing and detecting grain pollutants including ferromagnetic metal grains, non-ferromagnetic metal grains, water drops, bubbles and the like in oil liquid. According to the manufacturing method of the device, two plane coils and two solenoid coils are combined and embedded into a microfluidic chip for the first time based on a microfluidic chip technology; the two plane coils are laminated and the solenoid coils are symmetrically arranged at two sides respectively; two silicon steel sheets are added into the two solenoid coils respectively, so that the detection sensitivity of the device is remarkably improved. According to the chip, one sensor unit is used for carrying out voltage detection, so that various grain pollutants in lubricating oil and hydraulic oil are distinguished and detected;a feasible method is provided for online monitoring of the oil liquid through machine equipment and fault diagnosis can be carried out on the machine equipment.

The invention discloses a fluorescent probe capable of separately detecting cysteine / homocysteine, glutathione and sulfuretted hydrogen and a preparation method and application of the fluorescent probe. The molecular formula of the probe is C38H28IN5O5S, and the structural formula is shown in the description. The probe is simple to synthesize, higher in yield, can separately detect Cys / Hcy, GSH and H2S in a water solution, and can separately detect Cys / Hcy, GSH and H2S in the living cell level.