Fluorescent carbon quantum dot and preparation method and application thereof

Abstract

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is a nitrogen and phosphor-doped carbon quantum dot; a carbon source of the fluorescent carbon quantum dotis naphthalenedicarboxylic acid; the fluorescent carbon quantum dot has high yield rate of fluorescent quantum, can enter the microorganism to specially mark after the microorganism is deactivated, and the death and living of the microorganism can be effectively distinguished. The invention also discloses a preparation method of the fluorescent carbon quantum dot. The preparation method has the advantages that the raw materials are easy to obtain, and the preparation method is suitable for preparing the fluorescent carbon quantum dot with high yield rate of quantum. The invention discloses application of the fluorescent carbon quantum dot in activity detection of microorganisms and biomedical imaging; the fluorescent carbon quantum dot is used for effectively distinguishing the death and living of the microorganisms, so as to detect the activity of the microorganism at high efficiency; the cell toxicity of the fluorescent carbon quantum dot is low, and the fluorescent carbon quantum dot is suitable for in-vitro or in-vivo biomedical imaging detection.

Description

technical field [0001] The invention belongs to the technical field of carbon nanomaterials, and in particular relates to a fluorescent carbon quantum and its preparation method and application. Background technique [0002] Medical care and sanitation are important areas related to people's livelihood in my country. Among them, microbial activity is an important indicator for evaluating disease infection, environmental pollution, food safety, and antibacterial drug efficacy. In the pathogenicity detection of water, food, and clinical samples, antibacterial It has played an important role in drug development, disease prevention and control. Therefore, it is of great significance to develop simple, efficient and accurate detection methods for microbial activity. [0003] The traditional microbial activity detection mostly adopts the plate colony counting method, which is easy to operate and the detection results are reliable. However, the counting detection can only be carrie...

AI Technical Summary

Problems solved by technology

However, because the detection of nucleic acid requires expensive and precise instruments and takes a lot of time, and some detection methods are cumbersome and require professional operators, this greatly hinders the large-scale promotion and application of the market

[0004] Microscopic observation (including atomic force microscopy, electron microscopy, and fluorescence microscopy) and spectroscopic detection (including Raman spectroscopy and infrared spectroscopy) are one of the important means of microbial activity detection. However, sample preparation for atomic force microscopy and electron microscopy observation Complex and expensive equipment further limits its application

The observation results of the spectral detection method are also not intuitive enough, the follow-up data analysis is complicated, the data statistics are low, and the experimental design and data collection process are complicated

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