The invention relates to a
rosmarinic acid-containing
thromboplastin time measuring
reagent. The
rosmarinic acid-containing
thromboplastin time measuring
reagent is composed of a
Tris-HCl buffer containing 25 U / ml of
human thrombin, 2% of
bovine serum albumin, 1.5 mM of
rosmarinic acid, 0.05% of
sodium azide, 3.5% of
methionine, 1.5% of PEG, 3% of
mannitol, 2.5% of
maltose and 10 mM of
calcium chloride, wherein percentage is
mass-volume ratio, and a pH value is 7.4. A preparation method of the
reagent comprises the following steps: preparing the buffer with the pH value being 7.4 and
Tris concentration being 50 mM, adding BSA with final concentration being 2%, 1.5mM of rosmarinic acid with final concentration being 2%, triazacyclononane with final concentration being 0.05%,
methionine with final concentration being 3.5%, PEG with final concentration being 1.5%,
mannitol with final concentration being 3%,
maltose with final concentration being 2.5%, and 10mM of
calcium chloride in the buffer, wherein, besides
calcium chloride, the unit of the materials is
mass-
volume fraction; filtering a mixed liquor with a 0.22-
micrometer filter membrane, and adding a
human thrombin freeze-
drying preparation in a filtrate so that the concentration of
human thrombin in a final filtrate is 25 U / ml, and obtaining a TT reagent. According to the invention, rosmarinic acid is taken as a stabilizing agent, so that antioxidation performance and stabilization performance of the
thromboplastin time measuring reagent can be obviously increased.