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Chicken blood cell antioxidant peptide and its fermentation preparation method

A technology of anti-oxidative peptides and chicken blood cells, which is applied in the preparation methods of peptides, chemical instruments and methods, and fermentation, etc., can solve the problems of preparing high-activity antioxidant peptides that have not been reported, and achieve high-value utilization and optimization of fermentation. Condition, effect of high reducing power

Active Publication Date: 2020-09-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few studies on poultry blood at home and abroad, and the technology and products of preparing high-activity antioxidant peptides from fresh chicken blood have not been reported.

Method used

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  • Chicken blood cell antioxidant peptide and its fermentation preparation method
  • Chicken blood cell antioxidant peptide and its fermentation preparation method
  • Chicken blood cell antioxidant peptide and its fermentation preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] Example Preparation method of chicken blood cell antioxidant peptide

[0047] Include the following steps:

[0048] Collect fresh chicken blood, add 0.8% sodium citrate to the total blood weight, and centrifuge for 10 minutes with a high-speed centrifuge at a speed of 14,000 r / min to separate the chicken blood into plasma and blood cells.

[0049] Preparation of fermentation supernatant: use the hemocytes obtained above to prepare hemocyte medium (appropriate amount of hemoglobin, appropriate amount of glucose, 0.1% K 2 HPO 4 ·3H 2 O, 0.05% MgSO 4 ·7H 2 O, pH 7.0-7.2), sterilized at 121°C for 15 minutes. Add an appropriate amount of activated Bacillus natto into the cooled hemocyte culture medium, and place it in a constant temperature shaker at 37°C at 170rpm for culture. After the fermentation, centrifuge at a high speed of 5000×g for 10 min, and filter the supernatant with a 0.22 μm filter membrane to obtain the fermentation supernatant.

[0050] In order to i...

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Abstract

The invention provides chicken-blood-cell antioxidant peptide and a fermentation preparing method thereof. The method includes the following steps of (1) culture activation; (2) blood cell medium preparing; (3) inoculation fermentation; (4) fermentation liquor separation and purification. The amino acid sequence of antioxidant peptide is shown as SEQ ID NO:1. The chicken-blood-cell antioxidant peptide has the high free radical scavenging activity, has the high scavenging effect on DPPH free radicals, hydroxy free radicals and the like, has the high reducing capacity, and has the important value in the antioxidant activity development and application aspects.

Description

technical field [0001] The invention relates to the technical field of animal-derived antioxidant peptides, in particular to a chicken blood cell antioxidant peptide and a fermentation preparation method thereof. Background technique [0002] During aerobic metabolism, oxygen undergoes a series of reduction reactions to regulate signal transduction and homeostasis, accompanied by the formation of reactive oxygen species (ROS). Under normal conditions, the production of ROS and the antioxidant defense system are in a state of dynamic balance. Once the balance is broken, excessive ROS and other highly active molecules will lead to protein damage, DNA mutation, cell membrane phospholipid oxidation and low-density lipoprotein modification, resulting in oxidative damage to various organs and systems, thereby stimulating the production of harmful organisms. Diseases, such as broiler ascites syndrome, atherosclerosis, diabetes, cataracts, neurodegenerative diseases, cancer, etc. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/34C12P21/02
CPCC07K7/08C12P21/02
Inventor 张日俊郑召君黄燕李仲玄
Owner CHINA AGRI UNIV
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