The invention relates to a dyeing and finishing process for a cotton-plaited polyester one-way wet-permeable knitted fabric. The dyeing and finishing process for the cotton-plaited polyester one-way wet-permeable knitted fabric comprises the following specific steps: after only dyeing polyester of a cotton-plaited polyester knitted fabric in a dyeing machine, finishing the cotton-plaited polyesterknitted fabric by hair removal enzyme; and after finishing the cotton-plaited polyester knitted fabric by the hair removal enzyme, separately carrying out hydrophilic finishing and cotton surface water repellent finishing. The fabric has a one-way wet-permeable function, when a user takes strenuous exercises, sweat can rapidly permeate to the outside from the inner layer, thus, dryness and comfortableness of skin are maintained, bacteria are prevented from being bred, the fabric wears comfortably, and is suitable to be used as a fabric for autumn and winter sportswear or a fabric for school uniforms, moreover, dyeing defects such as polyester complexion, color spots, barriness and chicken claw grains can be avoided, dyeing difficulty is reduced, the dyeing effect tis improved, the dyeingquality is improved, dyeing is uniform, the function lasts for a long time, and the circumstance that color cannot be weaken sharply and even disappears along with increasing of washing times is avoided.