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57results about How to "Stable living environment" patented technology

Biological healthcare-type compound feed for soft-shelled turtles

The invention discloses a biological healthcare-type compound feed for soft-shelled turtles, wherein the material ingredients of the biological healthcare-type compound feed are as follows in percentage by mass: 30 to 40% of white fish meal, 10 to 20% of red fish meal, 4 to 10% of bulked bean pulp, 6 to 12% of yeast powder, 19 to 22% of starch a, 5 to 8% of fermented bean pulp, 2 to 5% of gluten powder, 2 to 5% of monocalcium phosphate, 0.05 to 0.3% of enzymic preparation, 0.1 to 0.3% of microbial preparation, 0.1 to 0.5% of compound vitamins, 1 to 3% of composite polymineral, 0.2 to 0.8% of 50% choline chloride, 0.1 to 0.5% of additive for protecting liver and gallbladder and 0.3 to 0.6% of additive for repairing intestinal mucosa. The feed has an increased feed utilization rate, and canbe used for improving the water body environment, reducing the disease occurrence and protecting the normal growth of the soft-shelled turtles, and thus, a high-quality and safe feed is provided for healthy cultivation.
Owner:ANHUI XIJIA AGRI DEV

Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof

The invention discloses a sodium alginate compound immobilized microbial agent and a preparation method thereof, and the sodium alginate compound immobilized microbial agent solves the problem that an immobilization way of pseudomonas suitable for di-chlorine quinoline acid degradation in the prior art is absent. A carrier culture medium with the pseudomonas and sodium alginate are mixed to obtain a mixture, and the mixture is dropwise added into calcium chloride with concentration being 1-4% to stand for 2-4 hours to prepare a granule; the mass concentration of the sodium alginate is 2-6%; the carrier culture medium comprises an adsorption carrier and an inorganic salt liquid culture medium; a weight ratio of the adsorption carrier to the sodium alginate is (1-3) to 1; and the adsorption carrier consists of corn cob, bamboo charcoal and oil cake in a weight ratio being 1:(1-2):1. According to the invention, the degradation efficiency of bacteria is effectively improved, and the degradation rate of the immobilized microbial agent is much higher than the sum of the physical absorption efficiency of blank spheres and biological degradation efficiency of a free bacterial strain, so that the effect of mutual promotion can be achieved.
Owner:四川润林肆汇中药材有限公司

Production technology of high-density composite nitrobacterium inoculant, and high-density fermentation batch culture fermentation tank system of nitrobacteria

The invention discloses a high-density batch culture production technology of a nitrobacterium inoculant for degrading ammonia nitrogen in water, and a high-density fermentation batch culture fermentation tank system of nitrobacteria, used for the technology. The production technology comprises the steps of magnetic nano-powder preparation, nitrobacterium seed liquid culture, magnetically carried nitrobacterium immobilization, batch fermentation culture, flocculation mixed precipitate magnetic separation, freeze drying and preparation compounding. The production technology improves the effective cell density of a nitrobacterium preparation, allows a flocculating agent and a stimulant to be compounded while preparing the nitrobacteria inoculant, and the nitrobacterium inoculant can be used in water restoration, can improve the transparence of water, and guarantees the sufficient nitrobacterium cell density required by water restoration and adaptability to new environment.
Owner:HUATIAN ENG & TECH CORP MCC

Mine explosion-proof pressure-resistant door

The invention relates to a mine explosion-proof pressure-resistant door which comprises a door frame and a door leaf, wherein the door leaf is arranged in the door frame; reinforcing rib plates are longitudinally and transversely arranged on the door leaf; sealing strips are arranged around the door leaf; a hand wheel mechanism is arranged on the door leaf; and the structure of the hand wheel mechanism is as follows: a mounting hole is formed on the door leaf; a copper sleeve is arranged on the mounting hole; a shaft sleeve is arranged on the periphery at the two ends of the copper sleeve respectively; the shaft sleeves are connected with the door leaf; a rotation shaft is sleeved in the shaft sleeve; and a pinion and a hand wheel are mounted on the rotation shaft respectively. The mine explosion-proof pressure-resistant door provided by the invention has reasonable structure and reliable performance, realizes various functions through a locking mechanism, a connecting rod mechanism and a hand wheel mechanism independently, and greatly improves the working efficiency, reliability and safety degree so that a cabin door can be started and closed in the shortest time when a safety accident happens to a coal mine.
Owner:无锡明浩船舶设备有限公司

Method for enrichment culture of Anammox

The present invention provides a method for enrichment culture of Anammox. According to the method, seven steps are performed to finally produce Anammox-rich active sludge, such that the MLSS in the anaerobic bioreactor is 20-35 g / L, and the sludge retention time in the anaerobic MBR membrane bioreactor (AnMBR) is up to 180-300 days, wherein a flow rate ratio of the anaerobic microorganism-rich liquid refluxing to the anaerobic bioreactor from the anaerobic MBR membrane bioreactor (AnMBR) to the effluent filtered by the MBR membrane bioreactor is 3:1. According to the present invention, with the method, no Anammox loss exists, the high-quality and stable living environment is provided for Anammox, Anammox is subjected to enrichment culture, and the anaerobic ammonium oxidation reaction starting time can be shortened to 6-8 months; and the method further has advantages of simple process, safety, stability, low treatment cost, small occupation area, short hydraulic retention time, and fast anaerobic ammonium oxidation reaction starting.
Owner:天津中冀源环保科技有限公司

Microfluidic cell culture chip guaranteeing uniform cell distribution and cell sample introduction method

The invention discloses a microfluidic cell culture chip guaranteeing uniform cell distribution and a cell sample introduction method. A main flow channel and auxiliary flow channels which are cross and vertical to the main flow channel are formed in a substrate, each auxiliary flow channel is a step-shaped flow channel which is granularly widened from the outer end to the middle, and the bottom surfaces of the auxiliary flow channels are higher than the bottom surface of the main flow channel; firstly, a culture solution is injected into a culture chamber, a cell suspension solution is injected into the culture chamber after the bottom of the culture chamber is filled with the culture solution, the culture solution is injected into the main flow channel after a certain quantity of the cell suspension solution is injected into the main flow channel, the culture solution pushes the injected cell suspension solution to the culture chamber, mixed gas enters buffer zones on two sides of the culture chamber through the auxiliary flow channels, the bottom of the culture chamber in the middle of the main flow channel is enabled to be filled with the cell suspension solution while the other space of the main flow channel is filled with the culture solution, therefore, cells are just distributed at the bottom of the culture chamber, no cells remain in the non-culture-chamber zone, and the cells are uniformly distributed at the bottom of the culture chamber.
Owner:南京九川科学技术有限公司

Deodorizing composite biological stuffing special for dyeing and finishing wastewater treatment and preparing method thereof

The invention discloses deodorizing composite biological stuffing special for dyeing and finishing wastewater treatment and a preparing method thereof. The stuffing comprises a component A and a component B. The mass ratio of the component A to the component B is 3:(6-8); the component A is prepared from dried sludge, converter slag, coal ash and clay, and the mass ratio of the dried sludge to the converter slag to the coal ash to the clay is (5-8):(5-7):(2-3):(3-5); the component B is prepared from, by mass, 10-20% of bark powder, 5-20% of saw dust, 50-60% of calcium carbonate and 20-30% of adhesive. The component A is prepared at first and then mixed with the component B to obtain the stuffing. The stuffing provides good adhesion space for growth of microorganisms, and provides a stable survival environment for growth of the microorganisms. Compared with inorganic stuffing like volcanic rock and natural or manual organic stuffing such as bark and synthetic resin, the stuffing has the advantages of being low in production cost, better in effect, stable in continuous use time, and beneficial for application to industrialized biological filter tank deodorization.
Owner:XINJIANG ENVIRONMENTAL ENG TECH

Culture apparatus and method for complete autotrophic nitrogen removal granule sludge

The invention discloses a culture apparatus and a method for complete autotrophic nitrogen removal granule sludge, relating to the technical field of biological sewage treatment, in particular to the treatment field of nitrogen pollution. The culture apparatus and the culture method are used for solving the problems that the anaerobic ammonia oxidation nitrogen removal method has low nitrogen removal efficiency and poor running stability due to low sludge concentration and poor sludge settling effect caused by small sludge flocculation particle size in the existing complete autotrophic nitrogen removal method based on anaerobic ammonia oxidation. The culture apparatus comprises a U-shaped reactor main body, a heat preservation system, a water inflowing system, a drainage system, an aerating system, an automatic control system and a parameter monitoring system. A situation that the whole culture process can be controlled and monitored intelligently is achieved by connection of all parts and setting of a culture environment, strong aeration is carried out firstly during culturing to enable the sludge to be granulated, and then anaerobic culture is performed on anaerobic ammonium oxidation bacteria to culture the complete autotrophic nitrogen removal granule sludge successfully. The culture apparatus and the culture method are suitable for the sewage treatment field.
Owner:HARBIN INST OF TECH

Vertical multistage AO ecological sewage treatment system

The invention relates to a vertical multistage AO ecological sewage treatment system. The vertical multistage AO ecological sewage treatment system is characterized in that sewage is orderly treated by a coarse screen, a lifting pump house, a fine screen, a grit basin, a vertical multistage AO ecological tank, a cloth filter tank and a UV disinfection canal and then is discharged by a standard discharge opening, and sludge produced by the fine screen, the grit basin, the vertical multistage AO ecological tank and the standard discharge opening is transferred into a sludge storage tank, then is treated in a dehydration machine room and can be carted. The treatment system has less sludge amount (of sludge-drainage time of 20-25 years), does not produce odor, realizes thorough removal of organic matters, provides a carbon source produced in sludge stabilization for denitrification demands, reduces an external carbon source, produces water satisfying first-grade standard A or B, can be used for construction of an ecological landscape park, has the advantages of less construction investment and short construction period, can utilize waste and old or existing pits, ponds, trenches and slopes, and has a construction period of 4-6 months.
Owner:FUJIAN GUANGHUILONG ENVIRONMENTAL PROTECTION TECH

Biogel for treating joint diseases and application thereof

The invention relates to biogel for treating joint diseases and application thereof. The biogel is prepared from biocompatible and degradable gel coated umbilical cord mesenchymal stem cells containing a special stem cell culture additive, and the stem cell culture additive is prepared from umbilical cord mesenchymal stem cells, traditional Chinese medicine extract and serum. The biogel disclosedby the invention can improve normal body cell proliferation, reduce effects of common negative stimulation of oxygen deficit, chemotherapy and the like to normal body cells, prolong cell growth time,promote cell factor secretion, effectively treat the joint diseases, remarkably reduce a fibrosis rate of skeletal muscle denervation fibrosis and reduce pathological tissue score of a knee osteoarthritis animal model. Furthermore, the medicine has the advantages of safety, high efficiency, small side effect, simpleness in production and low cost.
Owner:上海亚睿生物科技有限公司

Land-saving type efficient nitrogen and phosphorus removal sewage treatment device and method

The invention discloses a land-saving type efficient nitrogen and phosphorus removal sewage treatment device and method. The land-saving type efficient nitrogen and phosphorus removal sewage treatmentdevice comprises a pretreatment unit; the left end of the pretreatment unit is provided with a water inlet; the right end of the pretreatment unit is communicated with the left end of an anaerobic pool; the right end of the anaerobic pool is communicated with the left end of a first-stage aerobic pool; the right end of the first-stage aerobic pool is communicated with the left end of a secondarysedimentation pool; the right end of the secondary sedimentation pool is communicated with the left end of an anoxic pool; the right end of the anoxic pool is communicated with the left end of a secondary-stage aerobic pool; the right end of the secondary-stage aerobic pool is communicated with the left end of a deep treatment unit; the right end of the deep treatment unit is provided with a wateroutlet; and the anoxic pool and the secondary-stage aerobic pool are both filled with a biological membrane filler. The traditional A / A / O technology is improved into combination of front section A / Oadopting an activated sludge method and post A / O adopting a pure biological membrane method; and compared with the activated sludge method and a sludge-membrane mixing technology in the past, the combination greatly saves the occupying area while strengthening the pollutant effect.
Owner:四川天润德环境工程有限公司 +1

Preparation and application of ammonia oxidizing bacteria immobilized bioactive filler based on polyurethane carrier

The invention discloses preparation and an application of an ammonia oxidizing bacteria immobilized bioactive filler based on a polyurethane carrier, and belongs to the technical field of water treatment. The bioactive filler comprises two parts: an embedding body and the polyurethane carrier; a porous structure of the polyurethane foam carrier adsorbs a bacteria-containing embedding liquid, wherein the embedding liquid is prepared by mixing an ammonia oxidizing bacteria concentrated solution and a polyvinyl alcohol solution; a whole sheet of polyurethane foam is used as the embedding carrier, the embedding liquid is immersed to the interior of the polyurethane foam by a squeezing way, the embedding body is formed after boric acid secondary cross-linking and is combined with the polyurethane carrier to obtain a filler shape meeting requirements, and thus the ammonia oxidizing bacteria bioactive filler is obtained. The prepared ammonia oxidizing bacteria immobilized bioactive filler not only solves the problems that ammonia oxidizing bacteria are difficult in advantage establishment, easy to lose and the like, but also improves the processing ability of a reactor, shortens the start-up time, and has broad application prospects.
Owner:BEIJING UNIV OF TECH

Choice for preventing endogenic bacterial strain by potato

A select method for strain preventing and curing endotrophic mycorrhiza in potato. Detect width of bacteriostasis braid and select strains with good effects of endotrophic mycorrhiza, do pot incubation test and field practice, detect effects of prevention and cure and fix planting ability. Detect action of the strain to pathogenetic fungi, and induct antiviral ability to potato. Advanced strain is obtained after the steps.
Owner:重庆工学院

Biological gel for treating refractory skin damage and application of biological gel

The invention relates to biological gel for treating refractory skin damage and application of the biological gel. The biological gel is prepared from umbilical cord mesenchymal stem cells coated by biocompatible degradable gel with special stem cell culture additives, wherein the stem cell culture additives comprise umbilical cord mesenchymal stem cells, traditional Chinese medicine extracts and serum. The biological gel provided by the invention is capable of promoting normal body cell proliferation, reducing influence of common negative irritation such as anoxia and chemotherapy to normal body cells, prolonging cell growth time, promoting cell factor secretion and effectively treating refractory skin damage such as burning and scalding, and is safe and efficient, small in side effect, simple to product and low in cost.
Owner:上海亚睿生物科技有限公司

Local air-water reversing upflow biological aerated filter and sewage treatment method thereof

The invention discloses a local air-water reversing upflow biological aerated filter and a sewage treatment method thereof, and relates to a water treatment device and a water treatment method. According to the biological aerated filter, a needle punched nonwoven filter material is taken as a packing, and a plurality of aeration branch pipes are arranged in the height direction of an aeration main pipe. The water treatment method comprises the following steps of: regulating the pH value of sewage to be treated to be 7.5-8, pumping into the filter, pumping air into the filter, ensuring that the hydraulic retention time is 1 to 2 hours, and directly discharging the obtained supernate. Small-flow aeration of which the aeration direction is opposite to upward water flow, the water flow is disturbed on the water flow section, air and water are uniformly distributed, a mass transfer effect is good, phenomena of short flow and channeling can be reduced, anaerobic areas in a system can be effectively eliminated, a membrane is formed uniformly, a problem of poor nitrification effect during operation of the traditional biological aerated filter is solved to a great extent, and the average ammonia nitrogen concentration of effluent is 8.63mg / L. The invention is used for treating wastewater.
Owner:HARBIN INST OF TECH

Micro-fluidic gel gas-liquid interface flue gas exposing device

The invention discloses a micro-fluidic gel gas-liquid interface flue gas exposing device. The micro-fluidic gel gas-liquid interface flue gas exposing device comprises an upper-layer chip, a lower-layer chip and a middle-layer chip, wherein the upper-layer chip provides a flue gas inlet and outlet channel; the lower-layer chip provides a cell culture fluid flowing channel; the middle-layer chip is positioned between the upper-layer chip and the lower-layer chip and is sued for loading gel and cells on the gel; cell culture fluid in the lower-layer chip provides a living environment for cells in the middle-layer chip; and the gel in the middle-layer chip becomes a gas-liquid interface and enables the cells to be in direct contact with flue gas. The three layers of chips are combined, the cells are positioned on the gel and do not in direct contact with the cell culture fluid, and direct impact of the culture fluid to the surfaces of the cells is avoided; meanwhile, the culture fluid can be supplied for the cells through the gel, and stability of the living environment of the cells is kept; and on the basis, the cells can be in direct contact with flue gas, so that related biological information of the flue gas stimulating the cells is obtained.
Owner:NANJING UNIV +1

Bioreactor for treating high-salt organic wastewater based on composite salt-tolerant bacterial strain

The invention discloses a bioreactor for treating high-salt organic wastewater based on a composite salt-tolerant bacterial strain. The bioreactor comprises a plurality of anaerobic reactors and aerobic sewage treatment devices which are sequentially communicated in series through communicating pipes, a supporting pore plate is fixedly arranged at the lower end in each anaerobic reactor, a fillerparticle bed layer is laid on each supporting pore plate, salt-tolerant anaerobic bacteria are added into each anaerobic reactor, and a first water distribution pipe is laid at the bottom in each anaerobic reactor; when every two adjacent anaerobic reactors are communicated in series through a communicating pipe fitting, a water filter screen is arranged at an inlet in the front end of the communicating pipe fitting and horizontally extends into the upper end of a previous anaerobic reactor, and an outlet in the rear end of the communicating pipe fitting is communicated with the first water distribution pipe laid at the bottom of the interior of a next anaerobic reactor; after anaerobic treatment, the wastewater flows into an aerobic sewage treatment device for aerobic treatment. The bioreactor disclosed by the invention is relatively high in high-salt organic wastewater treatment efficiency and low in wastewater treatment cost.
Owner:浙江省天正设计工程有限公司

Plant configuration method for improving quantity and activity of wetland microbes

The invention discloses a plant configuration method for improving the quantity and the activity of wetland microbes. The method is characterized in that shallow-rooted wetland plants, medium-rooted wetland plants and deep-rooted wetland plants are planted in the matrix of a constructed wetland at intervals; and the root system of the shallow-rooted wetland plants is distributed in a 5-15cm plantation layer, the root system of the medium-rooted wetland plants is distributed in a 10-14cm plantation layer, and the root system of the deep-rooted wetland plants is distributed in a 20-60cm plantation layer. The plant configuration method allows the beneficial influences of the wetland plants on the wetland microbes to be maximally performed through reasonable layout of the kinds of the wetland plants on the basis of a wetland decontamination mechanism, effectively increases the quantity and the activity of the wetland microbes, and improves the wetland water treatment effect.
Owner:ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE +1

Bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis

The invention discloses bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis. The strain of the bacillus subtilis for preventing and treating bipolaris maydis is DZSY21, the preservation name is bacillus subtilis, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) which is located in #3, No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, the preservation date is November 27, 2015, and the preservation No. is CGMCC NO.11749. The 16S rDNA gene sequence of the bacillus subtilis is a nucleotide sequence as shown in SEQ ID No.1, and the gyrB gene sequence of the bacillus subtilis is a nucleotide sequence as shown in SEQ ID No.2. The bacillus subtilis is high in growth speed, large in sporulation quantity, good in stress resistance and good in antibacterial property, and can be quantitatively colonized in leaves of corn plants.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Preparation method of biological deodorant

PendingCN112516784AImprove the living environmentImproving the stable living environment of biological deodorizing bacteriaGas treatmentDispersed particle separationBiotechnologyBiochemical engineering
The invention discloses a preparation method of a biological deodorant, and belongs to the field of biology. The preparation method disclosed by the invention of the biological deodorant comprises thesteps of strain separation and purification, strain screening, strain compounding, loading preparation and the like. The invention solves the problems of complex preparation method of the existing biological deodorant, weak adaptive capacity of the prepared biological deodorant to the environment, poor action durability and unsatisfactory deodorization effect, and has the characteristics of highdeodorization efficiency and lasting deodorization performance.
Owner:苏州路易兴生物科技有限公司

Double sludge oxidation ditch denitrification phosphate-removing apparatus and method thereof

The invention discloses a double sludge oxidation ditch denitrification phosphate-removing apparatus and a method thereof. The apparatus comprises an oxidation ditch; an anaerobic zone, an anoxic zone and a water outlet zone which are communicated in order are arranged in the oxidation ditch, an independent nitrification zone is arranged between the anaerobic zone and the anoxic zone along the sewage flowing direction, a pre-anaerobic zone is arranged between the anoxic zone and the anaerobic zone, the independent nitrification zone is separated with the anaerobic zone and the anoxic zone through a first baffle plate and a second baffle plate, a first mud-separation zone, a filling material zone, a second mud-separation zone and a current stabilization zone are arranged in order along the sewage flowing direction, and nitrobacteria is enriched in the filling material zone. The denitrification phosphate-removing method is characterized in that a main channel of the oxidation ditch is always in an anaerobic / anoxic state, so that the denitrifying phosphorus-removing bacteria can be a dominant bacteria, the filling material is employed for hanging a membrane in the independent nitrification zone, so that a stable living environment is provided for nitrobacteria, nitrobacteria biomass in the independent nitrification zone is increased, nitrification rate is increased, and denitrification phosphate-removing effect can be guaranteed.
Owner:WUHAN UNIV OF TECH

Method for purifying water quality by constructing biodiversity in shallow water channels and ditches

The invention discloses a method for purifying water quality by constructing biodiversity in shallow water channels and ditches. The method comprises the steps of constructing a stepped water retaining dam, constructing natural revetments on both sides of a river bank and planting plants on slope soil layers on both sides of the river bank. According to the method, by utilizing processes such as physics, biology, water conservancy and the like, the water quality purification effect is remarkable and stable, and at the same time, through the combation with a beautification effect of artificialnatural landscape, the comfortable, safe and beautiful water environment concepts are fully embodied, and the method has a broad application prospect.
Owner:况红玲

Fish releasing device for water conservancy and hydropower engineering

The invention discloses a fish releasing device for water conservancy and hydropower engineering. The device comprises a pipeline and a fish carrying tank; a flow dividing pipe is arranged under the pipeline, and one side of the flow dividing pipe is provided with flow dividing through pipes; one side of the fish carrying tank is provided with an oxygen bottle, one side of vertical plates is provided with a push-pull plate, and the outer surface of the push-pull plate is provided with a control valve; the inner surface of the fish carrying tank is provided with a heat insulating layer, one side of the heat insulating layer is provided with a partition plate, and the partition plate is fixedly connected with the fish carrying tank through the heat insulating layer; two sides of the partition plate are each provided with buoyancy balls and flexible lines; and the outer surface of the heat insulating layer is provided with fixing bolts. According to the fish releasing device for the waterconservancy and hydropower engineering disclosed by the invention, an anti-slip mat layer, the buoyancy balls and the flexible lines are arranged, so that stability of the whole device can be increased, stability of the living environment of fishes can be maintained, and the fishes can be prevented from jumping out of the fish carrying tank and being hurt; and the device has good ventilation, issuitable for different working conditions, and brings better use prospects.
Owner:傅逅荣

Sea cucumber seedling culture water temperature constant system and use method thereof

The invention discloses a sea cucumber seedling culture water temperature constant system and a using method thereof, and belongs to the technical field of mariculture. The sea cucumber seedling culture water temperature constant system comprises a first water inlet pipeline, a heating pipeline assembly, a cooling pipeline assembly, a second water inlet pipeline, and a control assembly; the heating pipeline assembly is mounted on one water outlet of the first water inlet pipeline; the cooling pipeline assembly is mounted on the other water outlet of the first water inlet pipeline; the second water inlet pipeline is connected with the heating pipeline assembly and the cooling pipeline assembly; and the control assembly is connected to the first water inlet pipeline, the heating pipeline assembly, the cooling pipeline assembly and the second water inlet pipeline and used for executing on-off of a water flow and regulation and control of the temperature of the water flow. According to the sea cucumber seedling culture water temperature constant system, the heating temperature and a stroke of the water flow can be calculated according to the temperature and humidity change of the surrounding environment, heat loss in the water flowing process is compensated, and the water temperature can be accurately controlled.
Owner:烟台市海洋经济研究院

Ecological cultivation method of domestic fish

The invention relates to an ecological cultivation method of domestic fish, which comprises the following steps of: 1) construction of a farm; 2) throwing fries; 3) running water management; 4) artificial feeding. According to the invention, a running water cultivation mode with a plurality of cultivation ponds with different depths which are communicated with each other is utilized, the method isof artificial natural ecological cultivation, promotes the growth of aquatic planktonic animals and plants, and maintains a good water cultivation environment. Meanwhile, scientific and nutritious artificial feed is prepared for feeding, and the cultivation yield of the fish is improved. By using scientific mixed cultivation ratio, the growth and living environments of the domestic fish are effective and stable and mutually promoted, and the cultivation quality is improved. The cultured domestic fish is delicious in flavor, good in taste, ecological, environment-friendly, low in cost and highin economic benefit, the method is suitable for large-scale production; meanwhile, chemical synthetic drugs and antibiotic drugs are not used, so that the produced fish has good quality and is safe to eat.
Owner:李宇

Nucleus pulposus progenitor cell culture medium and preparation method and application thereof

ActiveCN111019896AExcellent self-renewal abilitySimple stepsCulture processNervous system cellsTransferrinBiophysics
The invention provides a nucleus pulposus progenitor cell culture medium as well as a preparation method and an application thereof. The nucleus pulposus progenitor cell culture medium consists of a DMEM culture medium, an F12 culture medium, proline, sodium pyruvate, vitamin C, an insulin growth factor, FGF2, transferrin, a ROCK inhibitor, a p38 inhibitor and a transforming growth factor beta signal channel inhibitor. The preparation method comprises the following steps: mixing the components, adjusting the pH and osmotic pressure of the mixture, and performing sterilizing. The culture mediumdisclosed by the invention does not use animal-derived components; a undifferentiated and pluripotent state of the NPPCs can be maintained; the removal of animal serum in the culture medium greatly avoids the interference and pollution of animal components to nucleus pulposus progenitor cell culture, can completely avoid the induction of immune response in the cell treatment process, simplifies the culture process, increases the credibility of experimental results, and basically solves the key problem of durable culture of NPPCs. The preparation method is simple, raw materials are easy to obtain, and the culture medium is suitable for culture and amplification of nucleus pulposus progenitor cells under feeder-free cell conditions.
Owner:ZHEJIANG UNIV

Bacteriostatic odor-removing microbial preparation for pets and living environment of pets and preparation method and application thereof

InactiveCN111423999AWide range of energy sourcesAvoid competitive inhibitionBiocideFungiNutrientChemistry
The invention relates to the technical field of microbial preparations, in particular to a bacteriostatic odor-removing microbial preparation for pets and living environments of the pets. The microbial preparation of the invention comprises functional microbial agents of lactic acid bacteria, saccharomycetes and Nesterenkonia. The ratio of the colony count of the lactic acid bacteria, the saccharomycetes and the Nesterenkonia is (3-5):(8-11):(0.5-1.2). The invention also provides a preparation method and application of the bacteriostatic odor-removing microbial preparation for pets and livingenvironment of the pets. The microbial agent of the invention has better effect of removing odor molecules, can keep low consumption of nutrient substances, can reduce the harm caused by the accumulation of metabolites, and can maintain the performance of the product for a longer time due to the virtuous cycle.
Owner:SHENZHEN HE MIN BIOTECH CO LTD

Planting method of non-toxic rice

The invention provides a planting method for non-toxic rice, and belongs to the technical field of agriculture. The planting method comprises the following steps of preparing a base fertilizer, preparing a paddy field, carrying out paddy field arrangement, planting the rice, and carrying out normal field management, and also comprises the following steps of carrying out pretreatment on the paddy field, carrying out sowing, carrying out field management before seedling pulling, transplanting rice seedlings, carrying out field management in the growth period, carrying out treatment before harvest, and carrying out harvesting. According to the method, a traditional Chinese medicine is used for sterilization and deinsectization, so that the effects of sterilization and deinsectization are achieved under the condition that no chemical pesticides are used, meanwhile, a greenhouse and a temperature control and water control system are used for carrying out controlled planting on the rice in the paddy field, so that the interference of outdoor plant diseases and insect pests to the rice is isolated, meanwhile, the growth environment of the rice can be improved and automatically controlled,the yield of the rice is increased, in addition, a base fertilizer body and traditional Chinese medicine residues are adopted as the base fertilizer, so that soil is improved, salinization of the soil is avoided, and the traditional Chinese medicine residues can be further used as a fertilizer to promote the growth of the rice when being rotted and decomposed.
Owner:兴业县嘉沃生态农业科技有限责任公司

Trigona rearing box and application method

The invention discloses a trigona rearing box. The trigona rearing box comprises a box bottom, the top of the box bottom is provided with a pollen storage box, the top of the pollen storage box is provided with a breeding box, the top of the breeding box is provided with a honey storage box, and the top of the honey storage box is provided with a box cover. The top of the box cover is provided with a protection assembly with sun-proof and rain-proof functions, a first inner box layer is arranged in the pollen storage box, the portion between the inner wall of the pollen storage box and the outer wall of the first inner box layer is filled with a first thermal insulation layer, the inner wall of the first inner box layer is fixedly connected with a plurality of annular pollen storage plates, a second inner box layer is arranged in the breeding box, the portion between the inner wall of the breeding box and the outer wall of the second inner box layer is filled with a second thermal insulation layer, and a plastic honey box is arranged in the honey storage box. According to the trigona rearing box, a trigona comb is functionally partitioned by the pollen storage box, the breeding boxand the honey storage box, the multiple layers of boxes are combined and stacked, the boxes are disassembled to be recombined, and swarming is achieved. Honey is quickly produced through the honey box, and the damage to a swarm is reduced; airflow channels are formed, it is ensured that trigona is normally bred, the feeding box conforms to the life habits of the trigona, and therefore, the production efficiency is improved.
Owner:云南省农业科学院蚕桑蜜蜂研究所

A plant configuration method for increasing the quantity and activity of wetland microorganisms

The invention discloses a plant configuration method for improving the quantity and the activity of wetland microbes. The method is characterized in that shallow-rooted wetland plants, medium-rooted wetland plants and deep-rooted wetland plants are planted in the matrix of a constructed wetland at intervals; and the root system of the shallow-rooted wetland plants is distributed in a 5-15cm plantation layer, the root system of the medium-rooted wetland plants is distributed in a 10-14cm plantation layer, and the root system of the deep-rooted wetland plants is distributed in a 20-60cm plantation layer. The plant configuration method allows the beneficial influences of the wetland plants on the wetland microbes to be maximally performed through reasonable layout of the kinds of the wetland plants on the basis of a wetland decontamination mechanism, effectively increases the quantity and the activity of the wetland microbes, and improves the wetland water treatment effect.
Owner:ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE +1
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