72results about How to "Reduce selection pressure" patented technology

The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Fa and Cry1Da core toxin containing proteins in combination to delay or prevent development of resistance by the insect(s).

The invention provides a pesticide preparation for controlling vegetable pests of diamondback moth and the like, which relates to the technical field of pesticides. The main agent of the pesticide preparation is a mixture containing the following active ingredients in percentage by weight: 0.1-2.0% of nicouline and 0.1-50.0% of flubendiamide. The main agent and auxiliary agents are prepared into creams, microemulsions, aqueous emulsion and suspending agents. The pesticide preparation has the properties of good permeability, systemic property and the like, has high efficiency on the vegetable pests of diamondback moth and the like, can control the vegetable pests of diamondback moth and the like in different instars and thoroughly kill the pests, has the advantages of less pollution on theenvironment and low production cost, and is suitable for chemically controlling the vegetable pests of diamondback moth and the like.

The invention discloses a method for harmless treatment of ferment antibiotic fungus residues. The method includes: subjecting the ferment antibiotic fungus residues to ultrahigh-temperature intensive acidogenic fermentation treatment; performing biochemical treatment on the fungus residues subjected to ultrahigh-temperature intensive acidogenic fermentation treatment. Thermophilic hydrolysis acidogenic bacteria are adopted for high-temperature anaerobic acidogenic fermentation treatment of the ferment antibiotic fungus residues, the fungus residues are hydrolyzed and acidized, antibiotic producing strains in the fungus residues are inactivated, residual antibiotics are degraded prior to biochemical treatment, and accordingly harmless treatment and recycling of the antibiotic fungus residues are realized. By adoption of the method, the antibiotic producing strains in the fungus residues subjected to harmless treatment are completely killed, antibiotic residues are avoided, and the fungus residues subjected to biochemical treatment is free of dangers caused by drug-resistant fungi and drug-resistant genes. The method has advantages that efficiency in harmless treatment of the antibiotic fungus residues is improved, environment protection is benefited, hazardous wastes are recycled, and remarkable economic benefits and social benefits are achieved.

The invention discloses an integron In1069 with a sequence as shown in SEQ ID NO.1. The integron is found in a genome of drug-resistant Escherichia coli EC6335. The integron contains a plurality of drug-resistant gene cassettes. Therefore, due to the discovery of the integron disclosed by the invention, a certain guiding effect is provided for clinical medication, some clinically-used antibacterial agents can be favorably reduced, the selective pressure for horizontal transfer of the drug-resistant gene cassettes is reduced, and the outbreak of drug-resistant bacteria is avoided.

The invention discloses a pesticide combination comprising the active ingredients including fenazaquin and tebufenpyrad in a mass ratio of 60:1-1:60. By combining the fenazaquin and the tebufenpyrad, the combination has obvious synergy effect, can reduce the dosage of the active ingredients and the pesticide application frequency, and is conductive to retarding the drug resistance of mites. The combination of the invention can be used for controlling various mites on the crops and the horticultural crops, is especially suitable for controlling panonychus ulmi, tetranychus cinnabarinus and tetranychus urticae koch and can simultaneously control homopteran and hemiptera pests on the crops.

The invention discloses a pretreatment method of ferment antibiotic fungi residues. The pretreatment method comprises ultrahigh temperature reinforced acidogenic fermentation treatment of the ferment antibiotic fungi residues. Thermophilic hydrolysis acid-forming bacteria are used for performing high temperature anaerobic digestion treatment on the ferment antibiotic fungi residues, so that the fungi residues are hydrolyzed and acidified, antibiotic producing bacteria in the fungi residues are inactivated, and retained antibiotics are degraded. The fungi residues pretreated through the method can be directly subjected to subsequent biochemical treatment after being subjected to antibiotic removal. According to the method disclosed by the invention, the antibiotic producing bacteria can be completely killed, the antibiotics in the fungi residues can be removed, inhibition of high-concentration antibiotics to microorganisms is reduced, the difficulty in treatment of the fungi residues based on the subsequent biochemical method is reduced, generation of drug-resistant fungi and drug-resistant genes in the subsequent biochemical treatment is reduced, the harmless treatment efficiency of the antibiotic fungi residues is improved, and environmental protection is facilitated.

The invention discloses a retarder combining Chinese medicines and western medicines and used for preventing and curing the rabbit coccidiosis and a preparation method thereof, pertaining to the technical field of prevention and cure with the drug of poultry and livestock parasite. The retarder comprises ingredients with portions by weight: 2 to 5 portions of antifeverile dichroa root, 3 to 6 portions of abrotanum, 2 to 5 portions of sophora flavescens, 1 to 3 portions of pulsatilla chinensis, 1 to 3 portions of radix bupleuri, 1 to 3 portions of rhizoma imperatae, 1 to 3 portions of liquoric root, 0.01 to 0.05 portions of diclazuril, 8 to 10 portions of porous starch and 0.001 to 0.003 portions of chitosan. The ingredients are prepared by the steps of soaking in ethanol and Soxhlet extraction, followed by soaking in water and Soxhlet extraction, mixing, absorption and combination of extract and diclazuril as well as porous starch and chitosan, and regulation of medicinal powder quality, etc. The results from the experiments show that the egg reduction rate for the rabbit coccidiosis is 99.2 percent; in addition, the retarder of the invention is mainly applied to the prevention and cure of the rabbit coccidiosis.

A select method for strain preventing and curing endotrophic mycorrhiza in potato. Detect width of bacteriostasis braid and select strains with good effects of endotrophic mycorrhiza, do pot incubation test and field practice, detect effects of prevention and cure and fix planting ability. Detect action of the strain to pathogenetic fungi, and induct antiviral ability to potato. Advanced strain is obtained after the steps.

The invention relates to a hollow mesoporous silica nanoparticle loaded with rotenone and a preparation method thereof. The nanoparticle comprises an effective component and a nano-carrier, wherein the effective component is rotenone; and the rotenone accounts for 10.0% of the total weight of the nanoparticle, with the balance being the nano-carrier. According to the preparation method disclosed by the invention, a self-template synthesis method is adopted, and on the basis of synthesizing a solid mesoporous silica nanoparticle (HMSNs), water is used as an etching agent to prepare the hollow mesoporous silica nanoparticle (HMSNs). Researches show that the particle size of the prepared HMSNs is about 250 nm, and the specific surface area of the prepared HMSNs reaches 999.4 m<2> / g; and biogenic pesticide rotenone (Rot) is loaded into pores of the HMSNs through a solvent evaporation method, and the prepared rotenone-loaded nanoparticle (Rot-coated HMSNs) is uniform in particle size, has a drug loading rate of 46.7%, has good release characteristics and shows good prevention and treatment effects on imported cabbageworm larvae.

The invention relates to specific primers for PCR rapid detection of lactobacillus helveticus and a method. The primers are in a pair, wherein the nucleotide sequence of the forward primer is shown in SEQ ID NO.1, and the nucleotide sequence of the reverse primer is shown in SEQ ID NO.2. The invention also relates to the method for PCR rapid detection of the lactobacillus helveticus. The specific primers and the method have the advantages that the specific primers for PCR rapid detection of the lactobacillus helveticus are obtained for the first time by adopting the non-coding sequence of the genome of the lactobacillus helveticus, and due to less selective pressure of the non-coding sequence in the genome evolution and poor conservation among close species, the specificity of amplified products is better and the accuracy is higher.

The invention relates to a pesticide formulation for preventing and controlling green peach aphids, and in particular relates to an insecticidal pesticide formulation containing biopesticide-rotenone, belonging to the technical field of pesticides. The insecticidal pesticide formulation containing biopesticide-rotenone comprises the following ingredients by weight percentage: 0.1-20% of rotenone and 0.1-50.0% of sulfoxaflor, which are effective ingredients, and the balance of auxiliary agents. The pesticide formulation is in a form of microemulsion, emulsion in water or suspension concentrate. The pesticide formulation is in the form of microemulsion, emulsion in water or suspension concentrate prepared from the main ingredients, has the characteristics of good permeability, strong inabsorbability and the like, can thoroughly kill the green peach aphids in a high-efficiency manner, has low pollution to the environment and low production cost, and is suitable for chemical prevention of the green peach aphids.

The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all located at a single locus within the retroviral producer cell genome.

Compounds that inhibit bacterial virulence factors from the mono-ADP-ribosyltransferase (mART) family of toxins have been identified that are not toxic to cells or the producing bacterial pathogen. These compounds have great potential as antivirulence agents for treating many bacterial infections and disease states.

The invention relates to a captan and imazalil compound composition, which is excellent in environmental compatibility, high in efficiency and low in toxicity and residue, and application thereof, in particular to application researches on prevention and treatment of apple ring spot, apple anthracnose, grape white rot and grape bitter rot. The weight ratio of the active ingredients, captan and imazalil, in the compound composition is (1: 2)-(16: 1). The captan and imazalil compound composition can be used for preventing and treating major diseases of fruit trees and particularly is excellent in preventing and treating apple ring spot, apple anthracnose, grape white rot and grape bitter rot.

The invention discloses application of compositional composition of osthole and cardendazim in the field of agriculture, specifically aims at applied research of preventing and treating ring rot of apple.The present invention provides application of osthole and compositional composition of osthole and cardendazim in the field of agriculture, the weight ratio of osthole and cardendazim in the compositional composition is 1:25-40 with a preferable proportion of 1:30-40 and an optimum proportion of 1:35.The compositional composition of osthole and cardendazim is distinguished in the respect of preventing and treating ring rot of apple.

The invention discloses a new integron In0 with a sequence shown by SEQ ID NO.1. The integron is discovered in a genome of drug-resistant Escherichia coli EC6335. Therefore, on the one hand, the discovery of the integron disclosed by the invention has important theoretical and practical significances for discussing a molecular mechanism of bacterial drug resistance transfer from a gene level, and on the other hand, the discovery of the integron provides a certain guiding function for clinical medication, and is beneficial for clinically reducing the use of certain antibacterial drugs, reducing the selection pressure of horizontal transfer of drug-resistant gene cassettes, and preventing the explosive prevailing of drug-resistant bacteria.

The invention provides a method for efficiently screening saline-alkaline tolerant barley germplasm, which comprises the following steps of: firstly, performing saline-alkaline tolerant screening on barley germplasm resources in a seed germination period through a laboratory, greatly eliminating a batch of germplasm, and relieving field selection pressure; then selecting local representative saline-alkali soil, meanwhile, screening barley germplasm resources with high saline-alkali tolerance in the germination period in the field, and barley resources suitable for being planted in the local saline-alkali soil can be screened out. By means of the method, a batch of barley germplasm resources with high saline-alkaline tolerance suitable for local planting are selected and screened out withintwo years, and precious parent resources are provided for barley saline-alkaline tolerance breeding.

The invention discloses a novel integron In1289, wherein the sequence of the integron is shown as SEQ ID NO.1. The integron is discovered from a genome of drug-resistant nontyphoidal salmonella EC7328. Therefore, the integron disclosed by the invention, with discovery thereof, has important theoretical and practical meaning for discussing a molecular mechanism of bacterial drug resistance transfer at a gene level on one side, and on the other side, the integron has a certain guidance function for clinical medication; and the integron is conducive to reduce the application of some antibacterial agents in the clinical field, and the integron is capable of reducing selective pressure of horizontal transfer of the drug-resistant gene cassettes and preventing explosive epidemic of drug-resistant bacteria.

The invention relates to a pesticide composition containing fluquinconazole. An effective component is a compound composition of fluquinconazole and any metiram germicide, wherein the mass proportion of the two effective components is 40:1-1:80, preferably 10:1-1:10, and the balance is an allowed and acceptable auxiliary component in pesticide. The metiram germicide is selected from zineb, maneb, ambam, mancozeb, propineb, thioneb, and the like. After being compounded with any metiram germicide according to a certain proportion, the fluquinconazole has obvious synergy effect and can be used for preventing and controlling diseases of various plants, such as vegetables, fruit trees, grain, and the like.

The invention relates to a preparation for preventing and controlling spodoptera frugiperda based on biopesticide and a preparation method and application thereof, which belong to the field of plant protection and comprises the following active ingredients in percentage by weight: 0.1 to 10.0 percent of sophocarpidine, 0.1 to 10.0 percent of pyraquinil and the balance of adjuvant. The preparationmethod comprises the following steps of mixing the raw materials according to the weight ratio, and preparing a suspending agent by using a traditional suspending agent preparation method. The invention discloses application of the pesticide preparation. The suspending agent is prepared from the main agent, and the prepared preparation has the advantages of high efficiency on the spodoptera frugiperda, thorough killing, low environmental pollution and low production cost, and is suitable for green and efficient prevention and control of the spodoptera frugiperda.

The present invention discloses a novel integron In1290, which has a sequence represented by SEQ ID NO.1. According to the present invention, the integron is obtained by carry out restriction enzyme digestion sequencing identification in a Klebsiella Pneumoniae resistant strain; and the integron contains a plurality of drug resistant gene cassettes, such that the discovery of the integron provides the guidance for the clinical medication, the use of the antibacterial drugs is easily reduced in the clinic, the selection pressure of the horizontal transferring of the drug resistant gene cassettes can be reduced, and the explosive epidemic of the drug-resistant bacteria can be prevented.

The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; amplifiable selection marker; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all integrated at a single locus within the retroviral producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof. The nucleic acid vector additionally comprises nucleic acid sequences encoding an amplifiable selection marker. The invention also relates to uses and methods using said nucleic acid vector in order to produce stable retroviral packaging and producer cell lines.

The present invention discloses a pesticide main agent for controlling erythrina indica ichneumonid and preparation made up by using said main agent. Said main agent main agent mainly contains (by wt%) 5.0-40.0% of quinalphos and 0.1-10.0% of abamectin. Said main agent can be made into emulsifiable concentrate, microemulsion and emulsion in water.

The invention discloses application of a compound composition of eugenol and imazalil in the agricultural field, and particularly relates to application research special for prevention and control ofgrape white rot and anthracnose. The invention provides eugenol and application of the compound composition of eugenol and imazalil in prevention and treatment of grape white rot and anthracnose. In the compound composition, the weight ratio of eugenol to imazalil is 20:1-1:60, the synergism proportion is 1:(1-40), and the optimal proportion is 1:20. The compound composition has remarkable performance in the prevention and treatment of grape white rot and anthracnose.