Compound composition for preventing and treatment of grape diseases and application thereof
A compound composition, a technology for grape white rot, applied in the application, biocide, animal repellent and other directions, can solve the problem of drug resistance of bacteria, reduce the input amount, achieve remarkable control effect, and avoid drug resistance. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] Indoor toxicity test and compound ratio screening
[0023] 1.1 Drugs to be tested
[0024] 0.3% eugenol soluble agent, product of Shandong Yijia Agrochemical Co., Ltd., dissolved in water to make 1000mg / L mother liquor for later use.
[0025] 98% Imazalil original drug, provided by Yifan Biotechnology Group Co., Ltd. Dissolve it in acetone first, then make it into 10000 mg / L mother liquor at constant volume for later use.
[0026] 1.2 Tested strains and host plants
[0027] Grape white rot (Coniothyrium diplodiella) was isolated from diseased fruit in the field, and the first-generation strain obtained by monospore culture and purification was stored in a 4°C refrigerator for later use.
[0028] 1.3 Experimental method
[0029] The Mycelium growth rate test recommended by the FAO was used.
[0030] 1.3.1 Preparation of drug-containing tablet
[0031] 1.3.1.1 Drug compounding scheme
[0032] Table 1 Eugenol and Imazalil Compound Ratio
[0033]
[0034] Table 2...
Embodiment 2
[0063] field plot experiment
[0064] According to the indoor compounding ratio screening experiment, the optimal ratio of eugenol:imazalil=1:20 was selected, and we prepared 4.2% eugenol·imazalil composition.
[0065] 2.1 Experimental drug and community arrangement
[0066] According to the "Guidelines for Field Tests of Pesticides (2)" edited by the Ministry of Agriculture's Pesticide Control Institute, Part 121: Fungicides for Grape White Rot Control, "Part 13 of "Guidelines for Field Tests for Pesticides": Fungicides for Control of Grapes Anthracnose" designed a field efficacy test of 4.2% eugenol·imazalil on grape white rot and anthracnose.
[0067] 2.1.1 Drugs to be tested
[0068] 0.3% eugenol soluble solution 600 times, product of Shandong Yijia Agrochemical Co., Ltd.
[0069] 10% Imazalil water solution 800 times, provided by Belgium Janssen Pharmaceutical Company.
[0070] 4.2% Eugenol · Imazalil 800, 900, 1000 times, prepared by Yantai Academy of Agricultural Sc...
Embodiment 3
[0110] safety test
[0111] The content of imazalil in the compound composition is 6 percentage points lower than that of commercially available products, so its toxicity to humans and animals and the safety to crops meet the requirements; the content of eugenol in the compound composition is 0.2%. The results of the toxicity test of the accreditation agency show that the compound drug belongs to the slightly toxic pesticide:
[0112] Rat acute oral toxicity LD50♂>5000mg / kg,♀>5000mg / kg;
[0113] Rat acute dermal toxicity LD50♂>5000mg / kg,♀>5000mg / kg;
[0114] Rabbit eye irritation score is 0 (1h), after 48h it is 0 (1:100) dilution, no irritation to eyes;
[0115] Rabbit eye irritation score is 0 (4h), non-irritating to the skin.
[0116] The present invention establishes the optimal ratio of the two pesticides for use in production through indoor toxicity testing and field tests on the effects of the biological source pesticides eugenol and imazalil on grape white rot and a...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com