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Biogel for treating joint diseases and application thereof

A biological gel, joint disease technology, applied in the field of biomedicine, to achieve the effect of maintaining cell growth, prolonging the effective time, and prolonging the growth time

Inactive Publication Date: 2018-01-09
上海亚睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is currently no biological gel that can be used to treat joint diseases and has excellent curative effect, mechanical strength, safety and other aspects.

Method used

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  • Biogel for treating joint diseases and application thereof
  • Biogel for treating joint diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1 Chinese medicine extract (one)

[0048] 1. Accurately weigh the fully dried raw materials, including 8 parts of hibiscus flower, 3 parts of licorice, 15 parts of reed root, and 7 parts of chrysanthemum, soak them in 80% ethanol for 60 minutes, and grind them with a tissue breaker.

[0049] 2. Ultrasonic crushing, power 600W, time 30 minutes, 12000rpm centrifugation 15min, take the supernatant, and then dilute with ethanol.

[0050] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 5 times in total.

[0051] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.

[0052] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water until the t...

Embodiment 2

[0053] The preparation of embodiment 2 Chinese medicine extract (two)

[0054] 1. Accurately weigh the fully dried raw materials, including 10 parts of hibiscus flower, 5 parts of licorice, 10 parts of reed root, and 5 parts of chrysanthemum, soak them in 80% ethanol for 50 minutes, and grind them with a tissue breaker.

[0055] 2. Ultrasonic disruption, power 500W, time 40 minutes, 15000rpm centrifugation 10min, take the supernatant, and then dilute with ethanol.

[0056] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 5 times in total.

[0057] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.

[0058] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water until th...

Embodiment 3

[0059] The preparation of embodiment 3 Chinese medicine extract (three)

[0060] 1. Accurately weigh the fully dried raw materials, including 5 parts of hibiscus flower, 2 parts of licorice, 20 parts of reed root, and 10 parts of chrysanthemum, soak them in 80% ethanol for 70 minutes, and grind them with a tissue breaker.

[0061] 2. Ultrasonic crushing, power 600W, time 50 minutes, centrifugation at 10000rpm for 20min, take the supernatant, and then dilute with ethanol.

[0062] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 4 times in total;

[0063] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.

[0064] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water u...

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PUM

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Abstract

The invention relates to biogel for treating joint diseases and application thereof. The biogel is prepared from biocompatible and degradable gel coated umbilical cord mesenchymal stem cells containing a special stem cell culture additive, and the stem cell culture additive is prepared from umbilical cord mesenchymal stem cells, traditional Chinese medicine extract and serum. The biogel disclosedby the invention can improve normal body cell proliferation, reduce effects of common negative stimulation of oxygen deficit, chemotherapy and the like to normal body cells, prolong cell growth time,promote cell factor secretion, effectively treat the joint diseases, remarkably reduce a fibrosis rate of skeletal muscle denervation fibrosis and reduce pathological tissue score of a knee osteoarthritis animal model. Furthermore, the medicine has the advantages of safety, high efficiency, small side effect, simpleness in production and low cost.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a biological gel for treating joint diseases and its application. Background technique [0002] Stem cells are a kind of self-replicating pluripotent cells, among which mesenchymal stem cells are considered to have the most medical value. On the one hand, this kind of cells has the ability of self-replication and differentiation of stem cells, on the other hand, it is rich in various types of cytokines, which can not only regulate the level of local immunity, but also promote the regeneration of blood vessels and other body tissues, so as to effectively treat various tissue injuries and autoimmune diseases . Umbilical cord mesenchymal stem cells are considered to be the most functional cell type among them. This kind of cells not only has simple source, better tissue compatibility and stronger proliferation ability. [0003] At present, a large number of studies have shown ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/06A61K47/36A61K47/42A61K47/38A61K47/20A61P19/02A61P29/00C12N5/0775
Inventor 童强汪佳祺莫秀梅
Owner 上海亚睿生物科技有限公司
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