Microfluidic cell culture chip guaranteeing uniform cell distribution and cell sample introduction method

Abstract

The invention discloses a microfluidic cell culture chip guaranteeing uniform cell distribution and a cell sample introduction method. A main flow channel and auxiliary flow channels which are cross and vertical to the main flow channel are formed in a substrate, each auxiliary flow channel is a step-shaped flow channel which is granularly widened from the outer end to the middle, and the bottom surfaces of the auxiliary flow channels are higher than the bottom surface of the main flow channel; firstly, a culture solution is injected into a culture chamber, a cell suspension solution is injected into the culture chamber after the bottom of the culture chamber is filled with the culture solution, the culture solution is injected into the main flow channel after a certain quantity of the cell suspension solution is injected into the main flow channel, the culture solution pushes the injected cell suspension solution to the culture chamber, mixed gas enters buffer zones on two sides of the culture chamber through the auxiliary flow channels, the bottom of the culture chamber in the middle of the main flow channel is enabled to be filled with the cell suspension solution while the other space of the main flow channel is filled with the culture solution, therefore, cells are just distributed at the bottom of the culture chamber, no cells remain in the non-culture-chamber zone, and the cells are uniformly distributed at the bottom of the culture chamber.

Description

technical field [0001] The invention relates to the technical field of microfluidics and cell sampling, in particular to a microfluidic cell culture chip with evenly distributed cells and a cell sampling method. Background technique [0002] Cell culture refers to taking out tissues from living organisms, incubating and culturing them under in vitro conditions that simulate the physiological environment in vivo, so that they can survive and reproduce. Although traditional cell culture has been trying to create conditions to simulate the conditions in vivo, it has not been able to accurately reproduce the actual conditions of the extracellular microenvironment in vivo. Microfluidic chips can precisely control fluids in time and space, and cell culture on microfluidic chips has advantages. The uniformity of the distribution of cells in the culture chamber inside the microfluidic chip often leads to inconsistent growth of cells in different areas of the culture chamber. In th...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the shortcomings of the uneven distribution of existing cells in the microfluidic chip culture chamber, and to propose a microfluidic cell culture chip with evenly distributed cells and a cell sampling method. During the cell sampling process, Realize the uniform distribution of cells in the microfluidic chip culture chamber

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