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Biological gel for treating refractory skin damage and application of biological gel

A coagulant and material technology, applied in the field of biomedicine, to achieve the effect of promoting angiogenesis, prolonging the therapeutic effect, and maintaining cell growth

Active Publication Date: 2017-12-19
上海亚睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is currently no biological gel that can be used to treat refractory skin damage, and has excellent curative effect, mechanical strength, safety and other aspects.

Method used

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  • Biological gel for treating refractory skin damage and application of biological gel
  • Biological gel for treating refractory skin damage and application of biological gel
  • Biological gel for treating refractory skin damage and application of biological gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Chinese medicine extract of the present invention (1)

[0060] 1) Accurately weigh the fully dried materials, including 20g reed root, 10g blue lotus flower, 8g hibiscus flower, and 2g licorice, soak them in 80% ethanol for 60 minutes, and grind them with a tissue breaker;

[0061] 2) Ultrasonic crushing, power 600W, time 30 minutes, centrifugation at 12000rpm for 15min, take the supernatant, and then dilute with ethanol;

[0062] 3) Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 5 times in total;

[0063] 4) Drain the recovered n-butanol solution with a negative pressure centrifuge (-30mm Hg, 45 degrees Celsius), and dissolve the residue with absolute ethanol;

[0064] 5) Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water ...

Embodiment 2

[0065] Embodiment 2 Chinese medicine extract of the present invention (two)

[0066] 1) Accurately weigh the fully dried materials, including 15g reed root, 12g blue lotus flower, 6g hibiscus flower, and 3g licorice, soak them in 85% ethanol for 50 minutes, and grind them with a tissue breaker;

[0067] 2) Ultrasonic crushing, power 500W, time 40 minutes, centrifugation at 10000rpm for 20min, take the supernatant, and then dilute with ethanol;

[0068] 3) extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 4 times in total;

[0069] 4) Drain the recovered n-butanol solution with a negative pressure centrifuge (-40mm Hg, 50 degrees Celsius), and dissolve the residue with absolute ethanol;

[0070] 5) Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile wate...

Embodiment 3

[0071] Embodiment 3 Chinese medicine extract of the present invention (three)

[0072] 1) Accurately weigh the fully dried materials, including 25g reed root, 8g blue lotus flower, 8g hibiscus flower, and 1g licorice, soak them in 75% ethanol for 70 minutes, and grind them with a tissue breaker;

[0073] 2) Ultrasonic crushing, power 700W, time 20 minutes, centrifugation at 15000rpm for 10min, take the supernatant, and then dilute with ethanol;

[0074] 3) Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 6 times in total;

[0075] 4) Drain the recovered n-butanol solution with a negative pressure centrifuge (-20mm Hg, 40 degrees Celsius), and dissolve the residue with absolute ethanol;

[0076] 5) Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile wat...

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Abstract

The invention relates to biological gel for treating refractory skin damage and application of the biological gel. The biological gel is prepared from umbilical cord mesenchymal stem cells coated by biocompatible degradable gel with special stem cell culture additives, wherein the stem cell culture additives comprise umbilical cord mesenchymal stem cells, traditional Chinese medicine extracts and serum. The biological gel provided by the invention is capable of promoting normal body cell proliferation, reducing influence of common negative irritation such as anoxia and chemotherapy to normal body cells, prolonging cell growth time, promoting cell factor secretion and effectively treating refractory skin damage such as burning and scalding, and is safe and efficient, small in side effect, simple to product and low in cost.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a biological gel for treating refractory skin damage and its application. Background technique [0002] Refractory skin damage is not a disease, but a phenomenon of skin damage caused by various diseases or injuries. Common factors leading to refractory skin damage include burns, diabetes, lupus erythematosus, and psoriasis. At present, there is no perfect solution to these problems, because this kind of damage is often accompanied by complex immune disorders and tissue regeneration disorders, and a single treatment plan cannot solve all problems. However, stem cell therapy emerging in recent years is expected to solve all the problems of tissue regeneration and immune regulation. [0003] Stem cells are a kind of pluripotent cells with self-replication ability, among which mesenchymal stem cells are considered to have the most medical value. On the one hand, this kind of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61K9/06A61P17/02A61P3/10
CPCA61K9/0019A61K9/06A61K35/28C12N5/0668C12N2500/76C12N2500/84
Inventor 童强汪佳祺莫秀梅
Owner 上海亚睿生物科技有限公司
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