37results about How to "Save training space" patented technology

The invention discloses a detoxification and cell pan rapid propagation method for purple sweet potatoes and belongs to the technical field of crop variety breeding. The method comprises the following steps: disinfecting stem tips of the purple sweet potatoes; peeling and inoculating meristems of the stem tips of the purple sweet potatoes; culturing the meristems of the stem tips of the purple sweet potatoes; selecting the cell pan and a substrate; transplanting and rapidly propagating a virus-free tube seedling of the purple sweet potatoes and the like. According to the method disclosed by the invention, the formula of a detoxification and regeneration culture medium of purple sweet potatoes is improved, and the method is convenient to operate and great in inoculum size. By using the cell pan, the rapid propagation speed of a virus-free seedling is greatly accelerated. Furthermore, the method is high in propagation coefficient and is not limited by seasons.

The invention discloses a method for producing an inactivated vaccine of H9N2 subtype of avian influenza virus and a product of the inactivated vaccine, and belongs to the field of production and application of inactivated vaccines of H9N2 subtype of avian influenza virus. The method comprises the following steps: (1) culturing a MDCK cell by utilizing a cell factory; (2) inoculating H9N2 subtype of avian influenza virus, and proliferating virus in the cell factory, wherein the microbial preservation number of the H9N2 subtype of avian influenza virus is CGMCC No.9325; and (3) harvesting the virus, and preparing the inactivated vaccine. Immune protection tests prove that the protective rate of the H9N2 subtype of avian influenza virus produced by the method is 100 percent, and the antibody level of a cytotoxicity immunity group at each point in time is higher than that of a germ toxicity immunity group, and rises relatively high. Virus liquid produced by the method can be used for preparing medicines or reagents for preventing or treating H9N2 subtype of avian influenza virus.

The invention discloses a sports dancing barre suitable for any site. The sports dancing barre comprises a support rod, wherein the top of the support rod is provided with a cross rod; the support rod is sheathed with a casing pipe; the top end of the support rod is provided with a connection plug; the casing pipe is provided with a regulation screw; the casing pipe is connected with one end of a fixed rod through a spindle; the bottom end of the casing pipe is provided with a connection swivel pin; the connection swivel pin is connected with a fixed lug; the fixed lug is arranged on the fixed pin of the casing pipe; the surface of the fixed pin of the casing pipe is provided with a minitype operation touch screen and a USB (Universal Serial BUs) charging interface; a music player and a storage battery are arranged in the fixed pin of the casing pipe; one side of the fixed pin of the casing pipe is provided with an angle regulation motor; the angle regulation motor is connected with the fixed pin of the casing pipe through a flexible connecting rod. The sports dancing barre does not need to be installed on a wall surface so as to greatly save a training site and increase the learning of students, unnecessary resource waste is not caused, the sports dancing barre is convenient for carrying and is reasonable in design, and practicality is improved.

The invention discloses an efficient tilia miqueliana strain culture method which comprises the following steps: cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a primary culture medium, and performing culture; cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after seedling strengthening, cutting into appropriate micro cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a nutrient medium, and performing grown seedling culture on tilia miqueliana plants in a greenhouse. By adopting the method, the reproductive process, the rooting process and the strengthening process of tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the tilia miqueliana is increased, the survival rate of tissue culture seedlings is increased, the seed seedling proliferation process is accelerated, and the industrial seedling culture of the tilia miqueliana is facilitated.

The present invention is cultivation process of deactivated vaccine of attenuated polio strain. The cultivation process is completed with Vero cell and attenuated polio strain virus inside a fermentation tank. Through three stage amplification of cultivation scale, cell cultivating area equivalent to that of 21000 culturing glass bottle of 1 liter volume and cell output equivalent to that of 21000 culturing glass bottle of 1 liter volume may be obtained. The present invention can meet the requirement of large scale preparation of cell and virus liquid. The virus liquid of the present invention may be used to produce vaccine with stable quality, high safety and excellent immunogenicity.

The invention provides a combination type welding frame which comprises a column. At least one welding mechanism is arranged on the column and comprises an arm rod fixed with the column in bolt mode through a height location hole arranged on the column. A movable rod is arranged at the other end of the arm rod, and a clamp is fixedly connected with the other end of the movable rod. The combination type welding frame is simple in structure, flexible in assembly and capable of adjusting height and angle of a clamp and facilitates installation and welding operation of the clamp.

The invention relates to a rail transit driving simulation device, in particular to a simulation operation device enabling multiple types of simulation operation platforms to share a view displayer. The simulation operation device comprises an annular screen used as the view displayer, a simulation operation work area, simulation operation storage areas, a conversion device and simulation operation platforms. The annular screen is arranged in front of the simulation operation work area; the simulation operation storage areas are evenly distributed around the conversion device, and each simulation operation storage area is connected with the conversion device through tracks I; the conversion device is connected with the simulation operation work area through tracks II, and the space between the tracks I is the same as the space between the tracks II; small wheels capable of moving along the tracks are arranged at the bottoms of the simulation operation platforms, the simulation operation platforms are placed on the simulation operation storage areas when the simulation operation platforms do not work, and the simulation operation platforms are moved to the simulation operation work area through the conversion device when the simulation operation platforms work. According to the simulation operation device enabling the multiple types of simulation operation platforms to share the view displayer, multiple types of simulation operation platforms can share the view displayer, the problems that existing devices leave unused, utilization rate is low and repeated purchase are solved, capital is saved, and practical training places are saved.

The invention relates to a courtyard Chinese rose tissue culture seedling ex-vitro rooting method, which belongs to the technical field of Chinese rose propagation, and comprises the following steps: (1) matrix preparation and sterilization; (2) matrix filling preparation; (3) carrying out ex-vitro rooting culture; (4) sealing; and (5) culturing. According to the method, rooting culture of the courtyard roses is carried out in a mode of combining the ex-vitro rooting substrate and the 126-hole tray, so that the tissue culture procedure is simplified, the tissue culture space is saved, the use of plant hormones is reduced, and the rooting rate is relatively high.

The invention discloses an intermittent immersion type orchid protocorm-like propagation method, which comprises the following steps: carrying out aseptic culture of explants to induce orchid protocorm (PLB); 2) the step 1) The obtained PLB is placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture; the present invention has the advantages of high utilization of the culture space and is conducive to the exchange of gas components in the culture, thereby improving the quality of the culture and the reproduction coefficient, suitable for large Large-scale production; culture equipment and accessories are easy to disinfect and sterilize as a whole, the culture process is fully enclosed, and the chance of contamination is small; the structure is simple and compact, and energy consumption is low, which saves culture space and reduces tissue culture costs; reduces the overall tissue culture process. The operation of transfer and subculture of seedlings saves labor and production costs.

The invention discloses a manufacturing method for an anaerobic atmosphere generation bag. The method comprises the following steps: (1) preparation of various ingredients: preparing the following ingredients in parts by weight: 1-100 parts of iron and magnesium ion, 1-100 parts of a carbonate, 10-100 parts of L-sodium ascorbate, 10-100 parts of powdered carbon, and 10-100 parts of agar; (2) mixing of various ingredients: stirring and uniformly grinding the various ingredients prepared in step (1), and sub-packaging; (3) packaging: finishing the preparation of a packaging bag provided with two-ply packs, wherein the inner pack is a ventilating bag and the outer pack is an air-proof bag; (4) manufacturing: uniformly stirring ingredients stirred and uniformly grinded in step (2), subpackaging in the inner packaging material according to required dosage by utilizing a packaging machine, and then conducting vacuum packaging rapidly by using the outer packaging material. According to the anaerobic atmosphere generation bag obtained through the adoption of the method, the operation is convenient, the reaction is rapid, the anaerobic atmosphere generation bag is convenient to use, the cultivation space is saved, the requirement for a cultivation vessel is low, and the manufacturing cost is greatly lowered.

The invention relates to a culture box for producing tissue engineering skin. The culture box comprises a bottom box with a liquid-changing groove, a box cover and culture brackets, wherein the culture brackets are spread in a single layer in the bottom box. When using the culture box of the invention, the box cover is only needed to be opened three times during the whole culture period so as to greatly reduce the probability that the product is polluted and largely increase the reliability during the culture process; during culture and production process, the culture space can be saved, the operation sequences can be reduced and the production efficiency can be increased so as to greatly reduce the production cost and realize mass production; the standardized production and operation are easy to realize and the product quality is very stable; and the culture box of the invention has lighter weight, is not easy to break up, and can realize automatic or semi-automatic manipulator operation during the culture and production of the tissue engineering skin, dramatically reduce the labor intensity, increase the production efficiency and provide the basis for the mass production of the tissue engineering skin.

The invention discloses a powder-free cold water fast-dissolving type micro-dried culture medium and a preparation method. The powder-free dried culture medium comprises culture medium dry powder which is dispersed in a film layer capable of absorbing water to form gel. The preparation process of the culture medium does not require boiling dissolving and high-pressure steam sterilization, and theproduction is simple and convenient. When the culture medium is used, no dust falls. Hydrophilic film forming matter and siphoning between the formed dry powder culture medium structure and an upper covering and a bottom culture medium jointly make sample liquid dispersed and absorbed quickly, the sample liquid is not spilled easily, and no second pollution is caused.

The invention relates to a rail transit driving simulation device, in particular to a simulation operation device enabling multiple types of simulation operation platforms to share a view displayer. The simulation operation device comprises an annular screen used as the view displayer, a simulation operation work area, simulation operation storage areas, a conversion device and simulation operation platforms. The annular screen is arranged in front of the simulation operation work area; the simulation operation storage areas are evenly distributed around the conversion device, and each simulation operation storage area is connected with the conversion device through tracks I; the conversion device is connected with the simulation operation work area through tracks II, and the space between the tracks I is the same as the space between the tracks II; small wheels capable of moving along the tracks are arranged at the bottoms of the simulation operation platforms, the simulation operation platforms are placed on the simulation operation storage areas when the simulation operation platforms do not work, and the simulation operation platforms are moved to the simulation operation work area through the conversion device when the simulation operation platforms work. According to the simulation operation device enabling the multiple types of simulation operation platforms to share the view displayer, multiple types of simulation operation platforms can share the view displayer, the problems that existing devices leave unused, utilization rate is low and repeated purchase are solved, capital is saved, and practical training places are saved.

An ECG signal classification method based on the combination of CNN and GRU in the self-encoding mode, by extracting the most representative features in the original signal, using CNN+GRU for feature extraction, saving space and saving a lot of training space, among which The GRU (Gated Recurrent Unit) used on the one hand solves the problem of gradient disappearance and gradient explosion due to RNN training. On the other hand, it has one less gate than LSTM, which is easier to calculate and can improve training efficiency. The advantage of GRU is that when When there are few training samples, it can be used to prevent overfitting. When there are many training samples, it can also save a lot of training time, and can improve the learning efficiency of the network and the accuracy of ECG signal recognition.

The invention discloses an incubator. The incubator comprises a tray and a dividing fence which is arranged in the tray, used for dividing the tray into multiple grids and crossly formed by multiple mutually parallel cross baffles and multiple mutually parallel vertical baffles; and a gap for connecting the multiple grids exists between the lower end face of the dividing fence and the inner bottom face of the tray. The dividing fence in the incubator provided by the invention is used for dividing the tray into the multiple grids communicated with the bottom and solid culture mediums are distributed on the whole tray through the gap on the bottom of the dividing fence; each solid culture medium is higher than the bottom of the dividing fence, so that the solid culture grids with great number, same height and relatively independent space are formed, the bacterial culture flux is increased, the culture space is reduced, and the bacterium is easily marked.

The invention discloses a method for multiplying and rapidly multiplying fern green spheroids by intermittent immersion, which comprises the following steps: 1) aseptically culturing to obtain fern green spheroids; 2) making the green spheroids obtained in step 1) The body is placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.

The invention discloses an intermittent immersion type lily scale multiplication and rapid propagation method, which comprises the following steps: 1) peeling off the scales of lily seed bulbs, and performing surface sterilization; 2) inoculating the surface sterilized lily scales on a solid base The culture medium is cultured for 7-10 days to carry out the selection of sterile explants, and then the lily scales which have been determined to be pollution-free are placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.