The invention discloses an efficient
tilia miqueliana strain culture method which comprises the following steps:
cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a
primary culture medium, and performing culture;
cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a
tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after
seedling strengthening,
cutting into appropriate micro
cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a
nutrient medium, and performing grown
seedling culture on
tilia miqueliana plants in a
greenhouse. By adopting the method, the
reproductive process, the rooting process and the strengthening process of
tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the
tilia miqueliana is increased, the
survival rate of
tissue culture seedlings is increased, the seed
seedling proliferation process is accelerated, and the industrial seedling culture of the
tilia miqueliana is facilitated.