Efficient tilia miqueliana strain culture method
A high-efficiency, linden plant technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of cumbersome domestication and transplanting of tissue cultured seedlings, low reproductive efficiency, low survival rate, etc., and achieve industrialized seedling cultivation , Simplify the breeding process and expand the breeding coefficient
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Embodiment 1
[0033] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:
[0034] (1) if figure 1 As shown, the current-year branches of the perennial Nanjing Tilia in the field were used as materials, and semi-lignified stems were cut as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculated on the primary culture medium , the germination induction of axillary buds was carried out in a constant temperature culture room with a temperature of 25°C, a light intensity of 2500lx, and a light cycle of 15h light / 9h dark; after 10 days of cultivation, the axillary buds germinated to about 1.0cm, as figure 2 As shown, the germination rate of axillary buds was 100%; wherein the primary medium was MS medium supplemented with 0.5mg / L TDZ, 0.25mg / L ZT, 30g / L sucrose and 7.0g / L agar.
[0035] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and ...
Embodiment 2
[0041] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:
[0042] (1) Using the current-year branches of the perennial Nanjing Tilia in the wild as materials, cut semi-lignified stems as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculate them on the primary culture medium In the medium, the germination induction of axillary buds was carried out in a constant temperature culture room with a temperature of 26°C, a light intensity of 3000lx, and a light cycle of 14h light / 10h dark; after 8 days of cultivation, the axillary buds germinated to about 1.0cm, and the germination rate of axillary buds was 100%; The primary culture medium is MS medium supplemented with 1.0mg / LTDZ, 0.5mg / L ZT, 30g / L sucrose and 7.0g / L agar.
[0043] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and carry out the proliferation and culture of t...
Embodiment 3
[0049] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:
[0050] (1) Using the current-year branches of the perennial Nanjing Tilia in the wild as materials, cut semi-lignified stems as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculate them on the primary culture medium The germination of axillary buds was induced in a constant temperature culture room with a temperature of 23°C, a light intensity of 2700lx, and a light cycle of 13h light / 11h dark; after 5 days of cultivation, the axillary buds germinated to about 1.0cm, and the germination rate of axillary buds was 100%; The primary medium is MS medium supplemented with 0.1mg / L TDZ, 0.05mg / L ZT, 30g / L sucrose and 7.0g / L agar.
[0051] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and carry out the proliferation and culture of the axillary buds in a constant te...
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