Method for identifying salt tolerance of barley in seedling stage
An identification method and technology of salt tolerance, applied in the field of identification of barley salt tolerance in seedling stage, can solve the problems of difficult salt concentration control, arduous engineering, large space occupation, etc., to ensure repeatability and accuracy, The effect of saving training space and saving manpower and material resources
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[0042]1. Select 20 barley materials as test materials, see Table 1 for details, wherein Numar, CM72, and C2118 are internationally recognized salt-tolerant varieties, and Franklin and Gairdner are recognized salt-sensitive varieties (references Zhou et al.2012.Angessa etal.2016), A7-20 is the dry seed of flower 30 60 After the radiation mutagenesis of Co 500GY, mutant materials were obtained by 500mg / L NaCl salt stress treatment. SP Hua 01-3 and SP Hua 03-2 were the materials for space radiation mutagenesis of varieties Hua 01-3 and Hua 03-2, respectively. Other materials are local varieties.
[0043] Table 1
[0044]
[0045] 2. Select barley seeds with full grains as the test material, pack them in gauze bags, dry them at 37°C for 30 minutes, wash them with tap water three times, put them in a greenhouse at 20±2°C, soak them for 6 hours, and wrap them with gauze , ensure proper amount of water, put in a 500ml beaker, seal the top of the beaker with a plastic film, tie i...
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