Yard rose tissue culture seedling ex-vitro rooting method

A technology for tissue culture seedlings and exogenous rooting, which is applied in the directions of botanical equipment and methods, cultivation, medium, etc., can solve the problems of low reproduction coefficient, less cuttings, and lower quality, so as to improve reproduction coefficient, simplify tissue culture procedures, The effect of improving the level of industrialization

Pending Publication Date: 2022-05-03
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commercial production of rose is mainly cutting, but there are few cuttings, and the reproduction coefficient is low. At the same time, the quality of seedlings propagated by cuttings is prone to decrease due to the accumulation of viruses.

Method used

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  • Yard rose tissue culture seedling ex-vitro rooting method
  • Yard rose tissue culture seedling ex-vitro rooting method
  • Yard rose tissue culture seedling ex-vitro rooting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for rooting out of a garden rose tissue culture seedling bottle, the method comprising the following steps:

[0023] (1) Irrigate the pure peat soil with water, fully moisten it, and keep the humidity at about 65%, put it into a plastic bag made of high-temperature-resistant materials, and sterilize it at 121°C and 1.01KPa for 40 minutes.

[0024] (2) Fill the matrix sterilized in step (1) into a 126-hole tray on the ultra-clean workbench, and compact it.

[0025] (3) Cut off the terminal buds of clustered sprouts that grow well in the bottle and have a seedling height of 5-6cm. The seedlings are 1-1.5cm long and are directly inserted into the sterilized subpackaged substrate in step (2). The tray was moved into the culture box, and 300 mL of sterile water was added along the edge of the box.

[0026] (4) Place the culture box on the sealing machine and seal it with 36 lines of laser perforated film.

[0027] (5) Place the culture box treated in step (4) in a...

Embodiment 2

[0029] A method for rooting out of a garden rose tissue culture seedling bottle, the method comprising the following steps:

[0030] (1) Irrigate the pure peat soil with water, fully moisten it, and keep the humidity at about 65%, put it into a plastic bag made of high-temperature-resistant materials, and sterilize it at 121°C and 1.01KPa for 40 minutes.

[0031] (2) Fill the matrix sterilized in step (1) into a 126-hole tray on the ultra-clean workbench, and compact it.

[0032] (3) Cut off the terminal buds of clustered sprouts that grow well in the bottle and have a seedling height of 5-6cm. The seedlings are 1-1.5cm long and are directly inserted into the sterilized subpackaged substrate in step (2). The dish was moved into the culture box, and 300 mL of sterile water was added along the edge of the box.

[0033] (4) Place the culture box on the sealing machine and seal it with 15 rows of laser perforated film.

[0034] (5) Place the culture box treated in step (4) in an...

Embodiment 3

[0036] A method for rooting out of a garden rose tissue culture seedling bottle, the method comprising the following steps:

[0037] (1) Pour the peat soil: perlite (volume ratio) = 1:1 matrix with water, fully moisten it, keep the humidity at about 65%, put it into a plastic bag made of high temperature resistant material, and store it at 121°C, 1.01 Sterilize at KPa for 40min.

[0038] (2) Fill the matrix sterilized in step (1) into a 126-hole tray on the ultra-clean workbench, and compact it.

[0039] (3) Cut off the terminal buds of clustered sprouts that grow well in the bottle and have a seedling height of 5-6cm. The seedlings are 1-1.5cm long and are directly inserted into the sterilized subpackaged substrate in step (2). The dish was moved into the culture box, and 300 mL of sterile water was added along the edge of the box.

[0040] (4) Place the culture box on the sealing machine and seal it with 36 lines of laser perforated film.

[0041] (5) Place the culture bo...

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Abstract

The invention relates to a courtyard Chinese rose tissue culture seedling ex-vitro rooting method, which belongs to the technical field of Chinese rose propagation, and comprises the following steps: (1) matrix preparation and sterilization; (2) matrix filling preparation; (3) carrying out ex-vitro rooting culture; (4) sealing; and (5) culturing. According to the method, rooting culture of the courtyard roses is carried out in a mode of combining the ex-vitro rooting substrate and the 126-hole tray, so that the tissue culture procedure is simplified, the tissue culture space is saved, the use of plant hormones is reduced, and the rooting rate is relatively high.

Description

technical field [0001] The invention belongs to the technical field of rose propagation, and in particular relates to a method for rooting out of a bottle of garden rose tissue culture seedlings. Background technique [0002] Rose chinensis Jacq is a plant of the genus Rosa in the family Rosaceae, and is known as the "Queen of Flowers". Due to its large flowers, elegant and beautiful flower shapes, and rich colors, the garden roses of Mayon Company are widely used in garden landscaping and garden beautification. At present, the commercial production of rose is mainly cutting, but there are few cuttings, and the reproduction coefficient is low. At the same time, the quality of seedlings propagated by cuttings is prone to decrease due to the accumulation of viruses. Tissue culture overcomes the disadvantages of low reproduction coefficient of conventional propagation methods, maintains the excellent traits of varieties, and obtains a large number of seedlings with consistent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/15A01G24/28
CPCA01G31/00A01G24/15A01G24/28
Inventor 许凤瞿素萍宋杰张艺萍王丽花杨秀梅苏艳张丽芳蒋亚莲
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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