A method for bulb induction and plant regeneration of Springgrass broadleaf through flower buds

A technology for spring grass and flower buds, applied in the field of plant tissue culture, can solve the problems of inducing broad-leaf spring grass bulbs and the like, and achieve the effects of overcoming the scarcity of female parents, overcoming the slowness of conventional propagation methods, and protecting the production of seedlings

Active Publication Date: 2016-08-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, there is no report on establishment of spring grass tissue culture before this, and there is no successful example of inducing spring grass bulb and plant regeneration through flower buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] The invention provides a method for inducing spring grass bulbs and plant regeneration through flower buds, the steps of which are:

[0041] 1), the preparation of culture medium

[0042] (1) Basic medium: MS medium, wherein sucrose 30g / L, agar 7.5g / L, pH=5.8;

[0043] (2) Induction medium: MS+6-BA4.0mg / L+NAA0.5mg / L;

[0044] (3) Proliferation medium: MS+6-BA0.5mg / L+NAA0.05mg / L;

[0045] (4) Strong seedling rooting medium: MS+6-BA0.01mg / L+NAA0.1mg / L.

[0046] 2), selection and sterilization of explants

[0047] Take the young inflorescences of Spring Grass, remove the outer covering of the closed flower buds on the inflorescences, cut off the flower buds and some scapes one by one, soak them in saturated washing powder solution for 60 minutes, then rinse them with tap water, and then wash them in volume Soak in 70% alcohol and 1% sodium hypochlorite solution for 1 min and 6 min respectively, and finally rinse with sterile water for 5 times; 3), induction and prolife...

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PUM

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Abstract

The invention discloses a method for inducing spring grass bulbs and regenerating plants through flower buds. The specific operation steps are as follows: 1) preparation of medium, including basic medium and components of medium for each stage of tissue culture; 2) Selection and disinfection of explants; 3) induction and proliferation of bulbs; 4) rooting of strong seedlings; 5) transplanting. Beneficial effects of the present invention: use plant tissue culture technology to directly induce bulbs and plant regeneration of spring grass flower buds, simplify tissue culture procedures, and obtain a large number of high-quality robusta with consistent genetic traits in a relatively short period of time. At the same time, it overcomes the shortcomings of the scarcity of the female parent and the slowness of conventional propagation methods, and has positive significance for the protection of spring grass germplasm resources and industrial seedling production.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for inducing spring grass bulbs and plant regeneration through flower buds. Background technique [0002] Albuca concordiana is a perennial ornamental succulent plant of the family Hyacinaceae. It is native to South Africa. Its ribbon-shaped leaves are twisted and twisted, and its spherical bulbs are simple and unique. Its flowers are fresh and elegant. It is a precious spring grass variety. Under natural conditions, spring grass grows slowly and has a low reproductive rate; at the same time, the International Organization for Succulent Plant Study (The International Organization for Succulent Plant Study) has formulated the "Succulent Plant Cultivation" in the 1970s in order to protect wild succulent plants from predatory collection or even extinction. "Knowledge and Code of Conduct for Collectors" has greatly restricted the introduction of succulents in my country, mak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王燕汪一婷牟豪杰吕永平陈志陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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