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A rapid regeneration tissue culture method of tartary buckwheat

A culture method and tissue regeneration technology, which is applied in the field of rapid regeneration tissue culture of tartary buckwheat, can solve the problems of difficult induction of regeneration shoots and low induction rate, and achieve the effects of reducing tissue culture costs, simplifying tissue culture procedures, and shortening regeneration time

Active Publication Date: 2017-12-19
四川西晨智慧农业科技有限公司 +1
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  • Application Information

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Problems solved by technology

[0003] The object of the present invention is to provide a rapid regeneration tissue culture method of tartary buckwheat, aiming to solve the problem of difficult induction of regeneration buds and low induction rate of some varieties of tartary buckwheat in the tissue culture regeneration process

Method used

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  • A rapid regeneration tissue culture method of tartary buckwheat

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preparation example Construction

[0053] Preparation of tartary buckwheat rapid regeneration explants: take 12-15 day-old tartary buckwheat aseptic seedlings, and cut the apical meristems of tartary buckwheat tissue culture seedlings in a workbench as explants.

[0054] Further, the hardening and transplanting of the regenerated tartary buckwheat plants: select the regenerated tartary buckwheat seedlings that grow robustly in the tissue culture bottle, open the bottle cap, wash off the medium on the adventitious roots, and transplant them into flowerpots filled with nutrient soil In the process, seedlings are hardened indoors for 4-6 days, and then the regenerated tartary buckwheat seedlings in the flower pots are taken out, and finally transplanted to soft soil for growth.

[0055] Further, the pH value of the MS basal medium is 5.8-6.2, and 0.25% plant gel is added at the same time.

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Abstract

The invention discloses a rapid regenerative tissue culture method of tartary buckwheat, which comprises four steps of cultivating aseptic tartary buckwheat seedlings, inducing clustered buds of tartary buckwheat, rooting of regenerated buds of tartary buckwheat, hardening seedlings and transplanting, and finally cultivating Complete tartary buckwheat regenerated plants. The invention has the advantages of strong applicability, simple and convenient operation, rapidity, economy and the like, and is a high-efficiency regeneration technology for rapidly propagating excellent germplasm of tartary buckwheat. The present invention mainly utilizes the totipotency of plant tissue cells, uses the apical meristem of tartary buckwheat as explants, induces culture in a specific medium, and regulates the intensity of environmental factors at the same time, and directly induces the production of clustered buds of tartary buckwheat and The rooting of buckwheat regeneration shoots, and then complete the rapid regeneration of tartary buckwheat.

Description

technical field [0001] The invention belongs to the technical field of tartary buckwheat tissue culture regeneration, in particular to a fast regeneration tissue culture method of tartary buckwheat. Background technique [0002] Buckwheat is a plant with the same origin as medicine and food, which can be divided into two categories: tartary buckwheat and sweet buckwheat. Among them, tartary buckwheat (F. tataricum Gaertn) belongs to Polygonaceae (Polygonaceae) and buckwheat (Fagopyrum) in taxonomy, and is an annual dicotyledons herbaceous plant. Tartary buckwheat originated in China. Its nature and flavor are bitter, flat, and cold. It is good for strength, spirit, eyes and ears, lowering qi, widening intestines and strengthening the stomach. It has extremely high nutritional and health functions. The seeds, roots, stems, leaves and other organs of tartary buckwheat are rich in flavonoids, which have the effect of "lowering blood sugar, lowering blood pressure, and lowering...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邵继荣朱雪梅王成龙周美亮吴燕民王应军杨远祥杨占彪程章
Owner 四川西晨智慧农业科技有限公司
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