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A kind of gerbera chrysanthemum tissue culture rapid propagation method

A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high cost, large differences in research results, high tissue culture costs, etc., and achieve tissue culture cost saving and reduction The transfer process and the effect of simplifying the tissue culture procedure

Inactive Publication Date: 2012-02-08
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gerbera is in the stage of rapid development in the international flower market. Production requires a large number of improved seedlings. Our country is in the promotion stage. The supply of improved seedlings is in short supply. The seeds and seedlings are all imported from abroad, and the cost is very high.
Many research institutes (institutes) have obtained regenerated plants through tissue culture, but most of them use the conventional "three-step seedling formation" method, that is, callus is induced from explants, and adventitious buds are induced from callus. , and then carry out three steps of induction of adventitious bud rooting, the cycle of its propagation seedlings is more than 7 months, and the price of gerbera seedlings is expensive due to the high cost of tissue culture
In addition, there are many varieties of Gerbera chrysanthemums, and the germplasm types are complex. Although the explant culture formula starting from the receptacle, petals, young leaves, stem tips, and leaf stems has been published, the research results of different varieties vary greatly, especially when using the same gerbera. It is difficult to realize the plant regeneration of gerbera green pistil and black pistil varieties with the formula of seed medium. For seedling production enterprises, it is still necessary to further optimize the tissue culture technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0014] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 2mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA3.0mg / L and IAA0.1mg / L Among them, the number of inoculations per bottle is 25;

[0015] (3) The explants inoculated in the culture flask were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 800Lux, light time of 14h / d, and temperature of 28°C. In 65 days, the length of the induction obtained was 4cm adventitious buds, wherein the induction rate of the green pistil variety was 99.8%, and the induction rate of the black pistil variety was 91%;

[0016] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.3mg / L, the number of inoculations per bottle is 10, under the conditions of 1500Lux lig...

Embodiment 2

[0019] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0020] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 4mm squares, and inoculate the leaves back down in MS medium supplemented with 6-BA 5.0 mg / L and IAA 0.3 mg / L , the number of inoculations per bottle is 10;

[0021] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under the conditions of light intensity of 1500Lux, light time of 13h / d, and temperature of 25°C under the conditions of green filter film. 50 days, the length that obtains induction is the adventitious bud of 8cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 97%;

[0022] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.2mg / L, the number of inoculations per bottle is 15, under the conditions of 800Lux ligh...

Embodiment 3

[0025] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0026] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 6mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA6.0mg / L and IAA0.4mg / L Among them, the number of inoculations per bottle is 15;

[0027] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 2000Lux, light time of 12h / d, and temperature of 20°C. 50 days, the length that obtains induction is the adventitious bud of 7cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 94%;

[0028] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.1mg / L, the number of inoculations per bottle is 20, under the conditions of 2000Lux light intensi...

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Abstract

The invention discloses a rapid propagation method of gerbera through tissue culture—the "two-step seedling formation method" of gerbera, which uses the leaves of test-tube seedlings as explants to directly induce adventitious buds and rooting culture to complete the production of gerbera tissue culture seedlings The whole process. Utilizing this method to produce gerbera seedlings not only solves the problem of difficulty in inducing adventitious buds and low induction rate of gerbera varieties with black pistils, but also breaks the boundaries between green gerbera varieties and black pistil varieties, which can be realized by one culture medium. More than 98% of the green pistil variety and more than 90% of the black pistil variety can efficiently induce adventitious buds; in addition, the present invention simplifies the tissue culture procedure, not only shortening the tissue culture cycle from the original 7 months to 4 months, but also reducing one transfer The cost of tissue culture is reduced by one-third.

Description

technical field [0001] The invention relates to the technical field of flower cultivation, in particular to a method for tissue culture and rapid propagation of gerbera - the "two-step seedling formation method" of gerbera. Background technique [0002] Gerbera (Gerbera jamesonii bolus), also known as gerbera, sun chrysanthemum, etc., is a perennial evergreen perennial herbaceous flower of the genus Gerbera in the family Asteraceae. The ornamental value of gerbera is high. Its flowers are huge, the diameter of the flower disc can reach 8-10cm, and the flower branches are tall and straight. It is favored by consumers because of the meaning of "supporting the husband and making the career prosperous". It has become the best-selling flower market in the world. One of the flower varieties. The backward propagation technology of gerbera is the main reason for the slow development of gerbera in my country. Gerbera is a cross-pollinated plant, which is self-fertile. Under natural ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王江勇杨娟侠王家喜王少敏王之涵
Owner SHANDONG INST OF POMOLOGY
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