DNA origami-based construction method and application of precise recognition targeted nano-carrier

Abstract

The invention relates to a DNA origami-based construction method and an application of a precise recognition targeted nano-carrier, and belongs to the technical field of medicines. The carrier is composed of scaffold DNA, staple single-stranded DNA, special staple single-stranded DNA and a nucleic acid aptamer, wherein a molar ratio of the scaffold DNA to the staple single-stranded DNA to the special staple single-stranded DNA is 1:(5-10):(5-10); a molar ratio of the nucleic acid aptamer to origami is 1:(1-32); a molar ratio of the carrier to an antitumor medicine doxorubicin during drug loading is 1:(12500-50000); and the appropriate drug loading time is 2-6 h. The targeted nano-carrier has the advantages of realization of a target heat accurately-modified NDA carrier, improvement of the targeting effect of the carrier and the target heat induced biological effects, delaying of the drug release after drug loading, reduction of the toxic and side effects of anthracene ring antitumor drugs, and enhancement of the antitumor effect on tumor cells, and makes a drug-loaded system simultaneously perform chemotherapy effects and biotherapy effects.

Description

technical field [0001] The invention belongs to the field of medical science and technology, and relates to the construction of anthracycline antineoplastic drug nanocarriers by DNA origami, in particular to a DNA origami-based construction method for precisely identifying and targeting nanocarriers and its application. Background technique [0002] DNA origami was first proposed by Nadrian Seeman in the 1980s. A milestone research achievement in this field is the research achievement published in Nature by Paul W.K.Rothemund in 2006. In the study, the synthesis steps of DNA origami were greatly simplified, so that the staples used in DNA origami no longer need to have a strict proportional relationship between the single-stranded DNA and the scaffold DNA, just excess, and the self-assembly process is also controlled by The previous tens of hours were shortened to hours or even tens of minutes. [0003] Nucleic acid aptamer is an oligonucleotide fragment with high affinity ...

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Problems solved by technology

[0005] Although the above-mentioned studies have improved the directional recognition of aptamers and their targets through some technical methods (such as linking with avidin or polymers through chemical bonds, etc.), increasing the stability and anti-tumor activity of aptamers, none of them A combination of nucleic acid aptamers that can be accurately quantified and positioned, so it is impossible to accurately identify and efficiently activate receptors that can cause tumor cell apoptosis (inhibit proliferation or migration, etc.), resulting in weak anti-tumor effects

[0006] The occurrence of malignant tumors is the result of the joint action of many factors, and most of the existing therapeutic drugs only target one target or act on a single factor

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