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Tissue culture and rapid propagation method for Gerbera jamesonii Bolus

A technology of tissue culture rapid propagation and medium, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of high cost, high tissue culture cost, large differences in research results, etc., and achieve tissue culture cost savings, Simplify tissue culture procedures and reduce the effects of transfer procedures

Inactive Publication Date: 2009-08-05
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gerbera is in the stage of rapid development in the international flower market. Production requires a large number of improved seedlings. Our country is in the promotion stage. The supply of improved seedlings is in short supply. The seeds and seedlings are all imported from abroad, and the cost is very high.
Many research institutes (institutes) have obtained regenerated plants through tissue culture, but most of them use the conventional "three-step seedling formation" method, that is, callus is induced from explants, and adventitious buds are induced from callus. , and then carry out three steps of induction of adventitious bud rooting, the cycle of its propagation seedlings is more than 7 months, and the price of gerbera seedlings is expensive due to the high cost of tissue culture
In addition, there are many varieties of Gerbera chrysanthemums, and the germplasm types are complex. Although the explant culture formula starting from the receptacle, petals, young leaves, stem tips, and leaf stems has been published, the research results of different varieties vary greatly, especially when using the same gerbera. It is difficult to realize the plant regeneration of gerbera green pistil and black pistil varieties with the formula of seed medium. For seedling production enterprises, it is still necessary to further optimize the tissue culture technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0014] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 2mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA3.0mg / L and IAA0.1mg / L Among them, the number of inoculations per bottle is 25;

[0015] (3) The explants inoculated in the culture flask were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 800Lux, light time of 14h / d, and temperature of 28°C. In 65 days, the length of the induction obtained was 4cm adventitious buds, wherein the induction rate of the green pistil variety was 99.8%, and the induction rate of the black pistil variety was 91%;

[0016] (4) Inoculate the obtained adventitious buds into MS medium with IAA 0.3mg / L, the number of inoculations per bottle is 10, under the conditions of 1500Lux light intens...

Embodiment 2

[0019] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0020] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 4mm squares, and inoculate the MS medium with 6-BA 5.0mg / L and IAA 0.3mg / L with the leaf back facing down , the number of inoculations per bottle is 10;

[0021] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under the conditions of light intensity of 1500Lux, light time of 13h / d, and temperature of 25°C under the conditions of green filter film. 50 days, the length that obtains induction is the adventitious bud of 8cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 97%;

[0022] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.2mg / L, the number of inoculations per bottle is 15, under the conditions of 800Lux light in...

Embodiment 3

[0025] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;

[0026] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 6mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA6.0mg / L and IAA0.4mg / L Among them, the number of inoculations per bottle is 15;

[0027] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 2000Lux, light time of 12h / d, and temperature of 20°C. 50 days, the length that obtains induction is the adventitious bud of 7cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 94%;

[0028] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.1mg / L, the number of inoculations per bottle is 20, under the conditions of 2000Lux light intensi...

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Abstract

The invention discloses a tissue culture and quick propagation method for flameray gerbera, and in particular relates to a flameray gerbera two-step seedling raising method. A leaf of a test-tube seedling is taken as an explant to directly induce an indefinite bud and carry out the rootage culture; and the whole process of the flameray gerbera tissue culture seedling is completed in two steps. The method which is used to produce the flameray gerbera germchit not only solves the problem that the flameray gerbera black pistil variety has difficulty in the indefinite bud induction and a low induction rate, but also breaks down the boundary between the flameray gerbera green pistil variety and the flameray gerbera black pistil variety, and realizes above 98 percent indefinite bud high-efficiency induction for the green pistil variety and above 90 percent indefinite high-efficiency induction for the black pistil variety through one culture medium. In addition, the method simplifies the tissue culture process. The method not only reduces the tissue culture period from originally 7 months to 4 months, but also reduces a shifting procedure. Therefore, the tissue culture cost is reduced by 1 / 3.

Description

technical field [0001] The invention relates to the technical field of flower cultivation, in particular to a method for tissue culture and rapid propagation of gerbera - the "two-step seedling formation method" of gerbera. Background technique [0002] Gerbera (Gerbera jamesonii bolus), also known as gerbera, sun chrysanthemum, etc., is a perennial evergreen perennial herbaceous flower of the genus Gerbera in the family Asteraceae. The ornamental value of gerbera is high. Its flowers are huge, the diameter of the flower disc can reach 8-10cm, and the flower branches are tall and straight. It is favored by consumers because of the meaning of "supporting the husband and making the career prosperous". It has become the best-selling flower market in the world. One of the flower varieties. The backward propagation technology of gerbera is the main reason for the slow development of gerbera in my country. Gerbera is a cross-pollinated plant, which is self-fertile. Under natural ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王江勇杨娟侠王家喜王少敏王之涵
Owner SHANDONG INST OF POMOLOGY
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