A kind of intermittent submerged lily scale multiplication and rapid propagation method

An immersion and intermittent technology, applied in the field of plant tissue culture, can solve the problems of neglecting and affecting the proliferation and rapid propagation efficiency of lily scales, and achieve the effects of saving culture space, easy overall disinfection and sterilization, and low energy consumption

Active Publication Date: 2017-09-22
INST OF BOTANY CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

However, the most important intermittent immersion culture device has not been introduced and improved, and the consideration of the most important limiting factor in the entire culture step, namely the intermittent immersion culture device, has been ignored, which has affected the proliferation and rapid propagation efficiency of lily scales to a certain extent. improvement

Method used

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  • A kind of intermittent submerged lily scale multiplication and rapid propagation method
  • A kind of intermittent submerged lily scale multiplication and rapid propagation method

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Experimental program
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Embodiment 1 100

[0037] Embodiment 1 lily bulb scale induced proliferation and rapid propagation culture

[0038] 1. First stack a plurality of empty object sieve trays in the fixed frame, then put the empty object component into the main body of the culture container, and then engage with the thread to seal the cover and the main body of the culture. In addition, the outer opening of the main body of the culture container is sealed with cotton balls, and the whole device is to be sterilized under high temperature and high pressure;

[0039] 2. The silicone hose can be sealed with newspaper and sterilized under high temperature and high pressure;

[0040] 3. Seal each opening of the liquid storage bag containing the liquid medium with cotton balls, and wait for high temperature and high pressure sterilization;

[0041] 4. The above-mentioned culture container device, load-carrying components and transportation accessories are sterilized by high temperature and high pressure, and placed on an ...

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Abstract

The invention discloses an intermittent immersion type lily scale multiplication and rapid propagation method, which comprises the following steps: 1) peeling off the scales of lily seed bulbs, and performing surface sterilization; 2) inoculating the surface sterilized lily scales on a solid base The culture medium is cultured for 7-10 days to carry out the selection of sterile explants, and then the lily scales which have been determined to be pollution-free are placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to an intermittent submerged lily scale multiplication and rapid propagation method. Background technique [0002] Plant tissue culture technology has the advantages of large and fast reproduction, easy control of experimental conditions, continuous production, and high production efficiency. It is widely used in crop breeding, germplasm resource preservation, and virus-free seedlings of flowers, fruit trees, vegetables, forest trees, and Chinese herbal medicines. Production and rapid propagation and vegetative propagation, such as the vegetative rapid propagation of lily virus-free seed balls. [0003] Traditional solid or semi-solid tissue culture methods consume a large amount of agar, and a large number of containers need to be handled during filling and cleaning, which consumes a lot of labor and leads to high production costs. [0004] At present, some plant tissue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 柴敏石雷李杨朱小佩
Owner INST OF BOTANY CHINESE ACAD OF SCI
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