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Incubator

A technology of incubators and trays, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of time-consuming, large cultivation space, and complicated identification, so as to reduce cultivation space and improve bacteria. Culture throughput, easy-to-identify effects

Inactive Publication Date: 2013-06-12
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of genetic engineering such as proteomics, the throughput requirements for the cloning and expression of recombinant genes are getting higher and higher, and a single solid culture plate cannot meet the high-throughput requirements. Complicated and other disadvantages

Method used

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Experimental program
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Effect test

Embodiment 1 96

[0028] The incubator of embodiment 1 ninety-six compartments cultivates bacteria

[0029] Put the divided grid with ninety-six grids into the tray, perform moist heat sterilization or high temperature dry baking sterilization, then place it on the ultra-clean table and slowly pour 1 liter of ampicillin-resistant sterile solid LB medium, Because there is a gap between the partition grid and the inner bottom surface of the tray, the culture medium can be distributed over the entire plate through the gap at the bottom of the partition grid, and finally cooled at room temperature to form a solid culture plate with 96 divisions.

[0030] Take an appropriate volume of DH5α bacteria solution (about 200 μL, 20-100 clones) transformed into the PET32a plasmid ligation product in the ultra-clean bench and add it to each compartment, shake gently to make the bacteria solution evenly distributed in each compartment, After blowing dry on the ultra-clean bench, put the lid on and incubate at...

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Abstract

The invention discloses an incubator. The incubator comprises a tray and a dividing fence which is arranged in the tray, used for dividing the tray into multiple grids and crossly formed by multiple mutually parallel cross baffles and multiple mutually parallel vertical baffles; and a gap for connecting the multiple grids exists between the lower end face of the dividing fence and the inner bottom face of the tray. The dividing fence in the incubator provided by the invention is used for dividing the tray into the multiple grids communicated with the bottom and solid culture mediums are distributed on the whole tray through the gap on the bottom of the dividing fence; each solid culture medium is higher than the bottom of the dividing fence, so that the solid culture grids with great number, same height and relatively independent space are formed, the bacterial culture flux is increased, the culture space is reduced, and the bacterium is easily marked.

Description

technical field [0001] The invention relates to the technical fields of bioengineering and bacterial culture. Specifically, the present invention relates to a culture device capable of providing bacterial culture flux, and in particular to an incubator. Background technique [0002] Bacterial culture is a routine technique in life science research, and it is one of the most basic and commonly used techniques in laboratories. It is mostly used for cloning and expression of recombinant genes in the field of genetic engineering. The traditional bacterial solid culture plate is a single circular culture plate with a diameter ranging from a few centimeters to tens of centimeters, and its material is glass and polystyrene. When cultivating bacteria, first pour the sterile solid medium into the flat plate sterilized by high pressure and humidity to form a highly suitable solid medium, then add an appropriate amount of bacterial liquid to spread evenly, and form bacterial clones th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/20
CPCC12M23/04C12M23/12C12M23/34
Inventor 王绪敏任鲁风冯杰邵长君李欣刚
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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