Method for efficiently transplanting betula platyphylla tissue culture seedlings
A technique for tissue culture seedlings and split leaves, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of occupying large tissue culture bottles and culture space, low transplanting efficiency, and low survival rate of tender branch cuttings, etc. problem, to achieve the effect of strong operability, improving the efficiency of multiplication, and saving space
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[0033] Embodiment 1 A kind of method for efficient transplanting of birch birch tissue culture seedlings
[0034] (1) Select the tissue culture adventitious buds of birch birch with good growth and inoculate them in the tissue culture bottle (bottle height 110mm, diameter 80mm) (see figure 1 ).
[0035] The medium for strong seedlings is: 2.14g / L WPM medium+0.56g / L calcium salt+20g / L sucrose+5g / L agar+1.5mmol / L NaOH+0.8~1.0mg / L 6-BA (purchased From Beijing Coolibo Technology Co., Ltd.). The temperature of the culture room was kept at 25°C ± 2°C, the light was 16h / dark for 8h, and the light intensity was 4000-5000Lx. With the extension of the culture time, the sub-seedlings gradually differentiated into clump seedlings, and the height of the seedlings gradually grew. After about 40 days of culture, the height of the seedlings was about 3cm to 5cm.
[0036] (2) Preparation of rooting substrate
[0037] ①First, prepare the matrix according to the ratio of V (peat soil): V (ve...
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