Method for efficiently transplanting betula platyphylla tissue culture seedlings

Abstract

The invention discloses a method for efficiently transplanting betula platyphylla tissue culture seedlings, and belongs to forest tree breeding. The method comprises the following steps: (1) inoculating tissue culture adventitious buds of betula platyphylla into a subculture strong seedling culture medium, and carrying out illumination culture to obtain clustered seedlings; (2) when the height of the clustered seedlings is 3-5cm, cutting off callus lumps at the lower parts of the clustered seedlings, then transferring the clustered seedlings into a rooting matrix of a tissue culture bottle, carrying out illumination culture, and after adventitious roots grow, carrying out step-by-step seedling hardening and transplanting; wherein 20-30 clustered seedlings are inoculated in each bottle of rooting substrate. The tissue culture process provided by the invention is high in operability, and a large number of betula platyphylla seedlings can be produced in a tissue culture room, which also plays a reference role in transplanting other forest tissue culture seedlings.

Description

technical field [0001] The invention relates to the field of forest tree breeding, in particular to a method for efficient transplanting of birch birch tissue culture seedlings. Background technique [0002] B. pendula 'Dalecarlica' is a new species from European white birch (Betula pendula Roth.). The trunk of this variety is white and straight, the branches are thin and soft and drooping, the growth is fast, and the resistance is strong. Greening has become an ideal tree species for landscaping in the alpine regions of Northeast China. The split-leaf birch is monoecious and dioecious, and the male flowers are aborted, which belongs to cross-pollination. Selected excellent individual plants can preserve their genetic characteristics only through vegetative propagation techniques. [0003] At present, the commonly used vegetative propagation methods of birch are mainly twig cuttings, tissue culture propagation and other technologies. Although the above method is also app...

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Problems solved by technology

Although the above method is also applicable to the vegetative propagation of birch, the survival rate of twig cuttings is relatively low, only about 57%.

Using peat, black soil, river sand and vermiculite mixed matrix instead of agar tissue culture propagation technology, because in order to ensure that the growth of seedlings is more robust, only one rooted seedling is cultivated in each bottle of mixed matrix, although the transplanted seedlings can reach nearly 100 % survival rate, however, this method needs to take up a lot of tissue culture bottles and culture space, resulting in the problem of low transplanting efficiency

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