A kind of powder-free cold water instant micro-dry medium and its preparation method

A drying medium and culture medium technology, which is applied in the field of powder-free cold water instant micro-drying medium and preparation, can solve the problems of inaccurate test results, pollution of operating space, slow absorption and diffusion, etc., and achieve low cleanliness and small volume , the effect of stable quality

Active Publication Date: 2020-06-16
GUANGDONG HUANKAI MICROBIAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this series of products are made of adhesive powder and coating, and dust will fall during use, causing secondary pollution to the inspected space environment, equipment and inoculation operation space.
In addition, due to the technical problems of the product itself, the absorption and diffusion of the sample liquid are slow after adding the sample liquid, and a pressure plate is required to spread the sample liquid and limit it within the specified range, and it is easy to overflow, resulting in inaccurate test results and pollution to the operating space.

Method used

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  • A kind of powder-free cold water instant micro-dry medium and its preparation method
  • A kind of powder-free cold water instant micro-dry medium and its preparation method
  • A kind of powder-free cold water instant micro-dry medium and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of powder-free dry medium

[0042] 1) 20g of tryptone, 10g of yeast extract, 4g of glucose, 5g of microcrystalline cellulose, and 80g of carrageenan, mixed by ball milling for 2 to 3 hours to make them uniform, to obtain dry medium powder;

[0043] 2) Add 2g of polyethylene glycol to 20ml of methanol and stir while fully dissolving and mixing. Weigh 1.5g of medium dry powder and add it to the above solution. Take about 2ml of polyethylene terephthalate (PET) with a diameter of 50mm. ) Blister test piece, hot air drying;

[0044] 3) After covering the polypropylene (PP) film, 15KGy irradiation sterilization, that is, the powder-free cold water instant micro-dry medium of the present invention.

Embodiment 2

[0045] Embodiment 2, the effect evaluation of the culture medium that acetyl triethyl citrate plasticizer is made

[0046] 1) Tryptone 5g, yeast extract powder 2.5g, glucose 1g and carrageenan 34g (more than 80 mesh), ball mill and mix evenly;

[0047] 2) Add different amounts of acetyl triethyl citrate to 20ml of methanol, then add 2g of polyethylene glycol, and after fully dissolving, add 1.5g of ball-milled dry powder to disperse evenly;

[0048] 3) Take 2ml and make it into a test piece and dry it;

[0049] 4) Sterilize by irradiation with cobalt 60 at a dose of 15KGy.

[0050] Escherichia coli ATCC25922, Enterobacter aerogenes ATCC13048, Citrobacter freundii ATCC43864, and Enterobacter cloacae CMCC45301 were cultured in the brain heart infusion at 36±1°C for 16-18 hours, and the enrichment solution was shaken with sterile saline Gradually dilute to about 100 CFU / ml, and finally add 1ml of 0.05% sterile TTC solution and mix well. Take 1ml and add it to the center of the...

Embodiment 3

[0055] Embodiment 3, the impact of surfactant Tween on the dispersion effect of dry medium

[0056] 1) Tryptone 5g, yeast extract 2.5g, glucose 1g, guar gum 41g, carrageenan 10g, ball mill and mix;

[0057] 2) Add 500ul of acetyl triethyl citrate to 20ml of methanol, then add 2g of polyethylene glycol to fully dissolve into a transparent solution, take 1.8g of the mixed dry powder and add it to the above solution and mix well;

[0058] 3) Take 2ml of one part and add it directly to a PET blister box with a diameter of 50mm, and heat it at 60°C until it is completely dry; add 200μl Tween 80 to the other part, mix well, take 2ml and add it to a PET blister box with a diameter of 50mm, 60 °C until completely dry. Observe the appearance of the medium formed after drying.

[0059] The results showed that the dried culture medium without adding Tween 80 was unevenly dispersed, in the shape of gully, and partly gathered together. The culture medium formed after adding Tween 80 aft...

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Abstract

The invention discloses a powder-free cold water fast-dissolving type micro-dried culture medium and a preparation method. The powder-free dried culture medium comprises culture medium dry powder which is dispersed in a film layer capable of absorbing water to form gel. The preparation process of the culture medium does not require boiling dissolving and high-pressure steam sterilization, and theproduction is simple and convenient. When the culture medium is used, no dust falls. Hydrophilic film forming matter and siphoning between the formed dry powder culture medium structure and an upper covering and a bottom culture medium jointly make sample liquid dispersed and absorbed quickly, the sample liquid is not spilled easily, and no second pollution is caused.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection and a preparation method thereof. Background technique [0002] In the 17th century, Anton van Leeuwenhoek and Robert Hooke successively discovered the existence of bacteria and filamentous fungi. Since then, the microscope and the culture method of microorganisms have become two urgent problems in the development of microbiology. In 1860, Pasteur used yeast waste residue, sugar and amino salt medium to cultivate bacteria in the laboratory for the first time. Agar was first discovered in 1658 by the Japanese Minora Tarazaemon. In 1882, Walter Hesse introduced agar to Koch, who became the first microbiologist to use agar. In the early 20th century, agar began to replace gelatin as a medium coagulant and has been used ever since. However, the agar medium needs to be heated and boiled above 85°C to dissolve, then sterilized by high-pressure steam at 121°C for 15 minutes, poured into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12Q1/10C12Q1/14C12R1/19C12R1/645C12R1/42C12R1/445C12R1/01
CPCC12Q1/04C12Q1/045C12Q1/10C12Q1/14
Inventor 滕昆仑吴清平卢勉飞陈博张建明蔡芷荷徐环
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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