The invention discloses a fresh fruit jelly and preparation method thereof. The raw materials of the fresh fruit jelly comprises: water, fresh pulp, golden syrup, white granulated sugar, composite glue, citric acid, sodium citrate, sweetening agent, essence, color protecting agent, anticorrisive agent and pigment; the preparation method of the fresh fruit jelly comprises the following steps: A, treating fresh fruits; B, making jelly material liquid; C, fusing the fresh fruit pieces into the jelly material liquid and obtaining the fresh fruit jelly. In the invention, the pulp treatment includes microwave heat treatment, dezymotizing and sterilization, the treatment time is 1 / 10 of the can heat treatment time, which maintains the fresh taste and flavor of the fruit and reduces the loss of nutrition components such as vitamins; and vacuum stain is used, which shortens time and the whole flow.