The invention provides a low-temperature mechanical perfusion preservation device and method, and the device comprises an organ box for placing an organ to be transplanted, a coolant container, a filter, a bubble catcher, a semiconductor refrigeration element, a pump, a perfusion loop, a circulation loop, a sensor assembly, an electromagnetic valve assembly, and a control display unit. Wherein the sensor assembly comprises a bubble sensor, a pressure sensor, a flow sensor and a temperature sensor, the coolant container is arranged on the periphery of the organ box in a surrounding mode, an open hole is formed in the side edge of the coolant container, and the temperature sensor detects the temperature of perfusate in the organ box through the open hole; the bubble catcher comprises three openings which are respectively a liquid inlet, a filling outlet and a circulating outlet, a partition plate is vertically arranged at the bottom of the bubble catcher, and the partition plate divides the bubble catcher into a plurality of communicated cavities; and the semiconductor refrigeration element is assembled outside the bubble catcher. The low-temperature mechanical perfusion storage device can directly detect the temperature of perfusate, the perfusate with the temperature exceeding the standard can be cooled to the set temperature range as soon as possible, bubbles can be prevented from entering a perfusion pipeline as far as possible, and the accuracy of perfusion data and perfusion safety are improved.