75results about How to "Less stringent" patented technology

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

The invention relates to compositions and methods for gene expression analysis. In some embodiments, the invention provides compositions and methods for amplifying targets in a degraded nucleic acid sample. In some embodiments, the invention provides methods for determining the quality of nucleic acids (e.g., the degree of degradation) in a nucleic acid sample. The invention also provides methods for producing a gene expression profile from a degraded RNA sample.

The present invention is related to a method for the identification and / or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and / or a quantification of the target compound.

This invention provides compositions and methods for HCV typing, e.g., genotyping and / or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV genotypes (for example, selected from genotypes 1, 2, 3, 4, 5 or 6), or assign an HCV isolate to one of at least six subtypes (for example, selected from subtypes 1a / b / c, 2a / c, 2b, 3a, 4a, 5a or 6a), where the methods of the invention use only a single typing probe to make the HCV type assignment.

The invention belongs to the technical field of rubber production, and particularly relates to a method for preparing butyl rubber. The method for preparing the butyl rubber comprises the following steps: (1) preparing, (2) polymerizing, and (3) terminating. Due to the adoption of the method, polymerization reaction can be performed in a less harsh temperature state, and the production cost and investment of high-class equipment are greatly reduced.

A method for constructing multiple nucleic acid sequences for use as positive controls in a genetic test is described. Compositions according to the invention including multiple nucleic acid sequences constructed as described are the optimal controls for simultaneously testing multiple variable nucleic acid sequences at one or more DNA or RNA sites in a subject or subjects. Sequences according to the invention can be prepared chemically and / or by PCR amplification for use directly or after cloning and propagation. At the same time, some sequences can be PCR amplified and / or cloned directly from total genomic DNA obtained from an individual carrying the mutation or variant. Alternatively, the normal sequence to be changed can be cloned and then modified by site directed mutagenesis. Several single mutant or polymorphic sequences that together comprise a panel of multiple control sequences can be added individually to single site tests or mixed together or ligated together by further PCR or by cloning into vectors prior to use in individual or multiplex tests. Controls sequences constructed according to the invention can be used when testing any genetically transmitted nucleic acid sequence by organizations testing quality assurance and by companies maintaining quality control of manufactured genetic test kits.

An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside alpha-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism.

The invention provides a method for preparing monocrystal graphene. Semiconductor layers are arranged on the upper surface and the lower surface of metal foil to obtain a semiconductor layer-metal foil-semiconductor layer stepped structure; the stepped structure is placed in a closed vessel of which air is emptied, the closed vessel is heated in the state that hydrogen and argon are introduced to enable the metal foil to be in a molten state, the metal foil can make contact with the semiconductor layers on the upper surface and the lower surface more closely, the contact area of the metal foil and methane is greatly decreased, therefore, the carbon source density of the surfaces of the metal foil is greatly reduced, and large-area growth of the monocrystal graphene can be facilitated. Due to the fact that the carbon source density of the surfaces of the metal foil can be greatly reduced by enabling the metal foil to closely make contact with the semiconductor layers on the upper surface and the lower surface, the vacuum degree requirement in the preparation method can be slightly lowered, the strictness for methane quantity control can be also lowered, and therefore the technology difficulty is lowered, so that the preparation process is implemented more easily.

The present invention relates to an advanced preparation method of organic-transition metal hydride used as hydrogen storage materials, the method including: preparing organic-transition metal-aluminum hydride complexes by reacting the organic-transition metal halide with metal aluminum hydride compounds; and preparing the organic-transition metal hydride by reacting the organic-transition metal aluminum hydride complexes with Lewis bases.Since the preparation method of the organic-transition metal hydride according to the present invention does not use catalysts, it has advantages that it does not cause problems due to poisoning and can prepare the organic-transition metal hydride at high yield under less stringent conditions. The hydrogen storage materials containing the organic-transition metal hydride prepared from the preparation method can safely and reversibly store a large amount of hydrogen.

The present invention provides isolated nucleic acids encoding insect G protein-coupled receptor (GPCR) polypeptides, isolated insect GPCR polypeptides, and uses thereof. Further provided are recombinant proteins and methods for identifying inhibitors to these proteins. The disclosed insect GPCR nucleic acids and polypeptides can be used in screening assays to identify modulating compounds. Protein inhibitors active in the methods disclosed herein are useful as insecticidal, ectoparasiticidal, antiparasitic, anthenenthic and acaracidal agents.

The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, At1g64090, At5g60690, At1g76350, At1g76580, At1g31930, At5g18230, At1g20970, or At4g35620.

The invention pertains to a novel method for analyzing activation pathways controlled by neurotransmitters and to a micro-array for use in this method. In particular, the present method relates to the use of such a micro-array as a tool for investigation of several activation pathways in the rat brain, said activation pathways being under the control of amine receptors.

Disclosed is a system for determining a protection level. The system includes a receiver configured to receive an error augmentation for a satellite orbit and clock error, an error augmentation for an ionospheric error, an error augmentation for noise and multi-path between a receiver and a satellite, and an error augmentation for a tropospheric error, a first calculator configured to calculate a first adjustment coefficient to be applied to the error augmentation for the satellite orbit and clock error and the error augmentation for the ionospheric error, and a second calculator configured to calculate a protection level by applying the first adjustment coefficient.

The invention provides methods and compositions for inducing necroptosis in target cells, including for example cancer cells. This invention provides compositions and methods for the controlled expression and formation of full length RDPK3 homodimers, truncated RTPK3 oligomers and / or full length RIPK3 / RIPK1 heterodimers in target cells both in vitro and in vivo therapeutic and research applications.

The present invention is related to a method for the identification and / or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of: putting into contact the target compound with a capture molecule in order in order to allow a specific binding between said target compound with a capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of said binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and / or a quantification of said target compound.

A radial turbomolecular vacuum pump that includes a rotor made from a silicon rotor surface comprising monolithically fabricated micro blades, and a stator made from a silicon stator surface comprising corresponding monolithically fabricated grooves. The micro blades and grooves are arranged in multiple rings, and the rotor and stator disks are placed in proximity, creating interdigitated stator and rotor blade rings. The interdigitated stator and rotor blade rings form a multi-stage compression in the radial direction.

The present invention relates to a system and method for controlling peptide display valency on virus-like particles (VLPs), especially including MS2 or PP7 VLPs. In this method, large amounts of wild-type and low quantities of single-chain dimer coat proteins may be produced from a single RNA. Valency is controlled in immunogen (vaccine) production by providing a system that allows the production of large amounts of wild-type and low quantities of single-chain dimer coating proteins from a single RNA, allowing facile adjustment of display valency levels on bacteriophage VLPs, especially MS2 or PP7 VLPs over a wide range, from few than one—on average—to as many as ninety per particle. This facilitates the production of immunogens and vaccines, including VLPs exhibiting low valency. Nucleic acid constructs useful in the expression of virus-like particles are disclosed, comprised of a coat polypeptide of bacteriophage such as MS2 or PP7 modified by insertion of a heterologous peptide, optionally comprising a carbohydrate mimotope, wherein the heterologous peptide is displayed on the virus-like particle and encapsidates bacteriphage mRNA.

A process for treating sulphide-containing water includes maintaining a steep redox potential gradient in an interface zone of the sulphide-containing water. The water is exposed to an oxygen-containing environment, and the interface zone is located immediately below the surface of the water. Sulphide in the water is biologically oxidized, in the interface zone, to sulphur. The sulphur may be removed by settling, thereby achieving a final removal of sulphur compounds.

The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyle-donous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root / kernel-preferential, leaf / endosperm-preferential, root / silk / kernel-preferential, or constitutive expression.

The invention provides a pipe bundle device and method for extracting isolated soybean protein in an ultrasonic-assisted manner.The pipe bundle device comprises a soybean meal premixing tank, a wet-method ultrafine grinder and an ultrasonic-assisted pipe bundle, wherein the upper end of the premixing tank comprises an alkali water pipeline, a purified water pipeline and a soybean meal conveying pipeline, and the lower end of the premixing tank is connected with the wet-method ultrafine grinder through a premixing conveying pipeline; the lower end of the wet-method ultrafine grinder is connected to the bottom of the ultrasonic-assisted pipe bundle through a crushed material conveying pipeline, a stirrer is arranged in the pipe bundle, and an ultrasonic generator and an extract conveying pipeline are arranged outside the pipe bundle.The pipe bundle device and method for extracting the isolated soybean protein is high in extraction efficiency, simple to operate and capable of considering both the extraction efficiency and extraction effect.

The present invention relates to expression cassettes comprising transcription regulating sequences with guard cell-preferential or guard cell-specific expression profiles in plants obtainable from Arabidopsis thaliana gene At5g58580, or from Arabidopsis genomic DNA sequences as described by SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, or 15.

The invention discloses a solvent thermal decomposition method for the preparation of a single crystal germanium nanowire. Trioctylamine is employed as high boiling point solvent to decompose an organic germanium precursor, and a germanium nanowire with micron grade length and single growth direction and controllable wire diameter ranging from 20 to 120 nm. The influences of different reaction time and different precursor concentration to the preparation of germanium nanowire are compared, and the methods for the preparations of different nanowires are summarized according to the experimental results. The preparation method is simple and can be easily controlled, and the prepared germanium nanowires have important application prospects in the design and manufacture of semiconductor devices and optic devices.

A package comprises a body, and an electrically conductive pattern supported by said body. An interface portion is configured to receive a module to a removable attachment with the package. The electrically conductive pattern comprises, at least partly within said interface portion, a wireless coupling pattern that constitutes one half of a wireless coupling arrangement.