32results about How to "Less sample required" patented technology

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

The invention discloses an electric automobile power battery SOC (State of Charge) detection system. The characteristics lie in that the detection system comprises a battery parameter acquisition platform and a battery SOC estimation system, the battery parameter acquisition platform is responsible for real-time parameter acquisition for the voltage, current and temperature of an automobile power battery pack, and the battery SOC estimation system can accurately estimate a battery SOC value through the acquired parameters; and the battery SOC is a non-linear, delayed, multivariable coupling and complex highly demanding real-time system. The detection system effectively solves a problem that the traditional automobile battery SOC estimation method is difficult to achieve an ideal effect.

The invention discloses a detection reagent containing nano mimic enzyme. The invention further discloses a visual detection method of bio-enzyme or protein and its inhibitor on the basis of nano mimic enzyme technology. The method shows good pH dependence for the oxidization reaction of many developing agents on the basis of some nano materials capable of simulating natural oxidase, and many oxidase or proteins can release / consume H+ characteristics in the biological catalysis process, and uses the in-site change of pH of reaction system to influence the catalytic oxidization ability of the nano material, thus the detection reagent has color change at different levels, and the visual detection of bio-enzyme or protein activity is realized, and further the visual detection of corresponding inhibitor is realized. The physical signal output by the detection method can show a very good linear relationship for the bio-enzyme or protein activity within a certain concentration scale, and thereby realizing the high-sensitivity detection of a target to be tested.

The invention provides a device for measuring the respiration of microbes. The device is equipped with a tubular reaction cavity, wherein, the inner wall of the tubular reaction cavity is equipped with a reaction sieve which divides the reaction cavity into an upper part and a lower part; the orifice of the tubular reaction cavity is equipped with a seal plug; the seal plug is equipped with a through hole; an electrode tip hermetically passes through the through hole to extend into the upper part of the tubular reaction cavity; the tubular reaction cavity is vertically placed in a thermostatic water bath, and the thermostatic water bath is equipped with a water inlet and a water outlet which are communicated with a thermostatic water tank for circulation; the bottom surface of the tubularreaction cavity is contacted with the bottom surface of the thermostatic water bath; the top of the thermostatic water bath is equipped with a hole which is hermetically matched with the external diameter of the orifice of the tubular reaction cavity; a magnetic sheet is placed on the bottom surface of the tubular reaction cavity; the bottom surface of the thermostatic water bath is placed on a magnetic stirrer; the tubular reaction cavity is filled with a reaction solution; and a biological sample is placed on the reaction sieve, and the sieve pore is smaller than the volume of the biological sample.

The invention discloses a strawberry greenhouse temperature intelligent detection device based on an empirical mode decomposition model, which is characterized in that: the detection device includes a strawberry greenhouse environment parameter acquisition platform based on a CAN field bus and a strawberry greenhouse temperature intelligent prediction system; the present invention It not only effectively solves many problems in the traditional strawberry greenhouse environment due to unreasonable design, backward equipment, and imperfect control system, but also effectively solves the existing strawberry greenhouse environmental monitoring system. Due to the characteristics of non-linearity, large lag, and complex temperature changes of the strawberry greenhouse environment, the temperature of the strawberry greenhouse environment is monitored and predicted, which greatly affects the regulation of the strawberry greenhouse environment temperature.

The invention relates to a high performance liquid chromatography method for jointly, rapidly and accurately detecting the contents of melanin and inosinic acid in chicken of silky fowl. The method comprises the following steps of: (1) carrying out protease oscillating enzymolysis on a chicken sample for 24 hours, centrifuging to obtain liquid supernatant which serves as inosinic acid to-be-detected solution; (2) orderly washing precipitate which is subjected to enzymolysis and centrifugation with diethyl ether, absolute ethyl alcohol and ultrapure water, removing lipid and impurities, so as to obtain a melanin crude product, oxidizing the crude product into 2, 3, 5-tricarboxylic acid pyrrole under alkaline condition, extracting with diethyl ether, and drying; and (3) dissolving the dry crystal with the to-be-detected inosinic acid solution, loading and detecting the sample on a high performance liquid chromatograph, and quantifying by an external standard method. The high performance liquid chromatography method only needs little sample to simultaneously detect the contents of the melanin and inosinic acid in the chicken sample of silky fowl, moreover, the method is simple in experimental operation steps, saves detection cost, and is beneficial for acceleration of screening excellent silky fowl breed (series) or combination with both outstanding functions and flavor substances.

Provided is an intelligent prediction system for a power battery SOC of an electric vehicle, the intelligent prediction system comprising a battery parameter collection platform and a battery SOC prediction system, wherein the battery parameter collection platform is used for collecting real-time parameters of the voltage, current and temperature of a vehicle power battery pack and an ambient temperature; and the battery SOC prediction system predicts, through the collected real-time parameters, a battery SOC value. The battery SOC is a real-time system which is non-linear, time-delayed, multi-variable coupled and complex, with high demands on real time performance. The intelligent prediction system effectively solves the problem that it is difficult for a conventional prediction device to obtain an ideal effect of battery SOC prediction accuracy.

The invention relates to the technical field of drug analysis, and specifically discloses a method for detecting related substances in rifampicin. The method comprises: preparing a test solution and a reference solution, and detecting by liquid chromatography-mass spectrometry, wherein the liquid chromatography adopts a C18 chromatographic column, and a formic acid aqueous solution with a volume concentration of 0.8‰~1.2‰ is used as a flow Phase A, using acetonitrile as mobile phase B, gradient elution; the mass spectrometer adopts ESI ion source, positive ion detection, SIM mode. The detection method provided by this application has the advantages of simple method, stability, high precision, good reproducibility, etc., and can quickly and accurately detect the impurities N-methylpiperazine and 1-amino-4- The whole operation process is reliable and controllable, suitable for practical application and promotion, and has broad application prospects.

The invention discloses a group of markers and a method for screening high-scent tea tree resources suitable for making oolong tea. The markers are composed of indole, nerolidol and jasmonolactone. The landmarks in the present invention can be applied to scoring and rapid screening of high-aroma tea tree resources suitable for making oolong tea. The method for screening high-flavor tea tree resources suitable for making oolong tea in the present invention has the advantages of simple operation, short time consumption, small sample size and high reliability, and can be applied to screening large quantities of resource samples.