32results about How to "Less sample required" patented technology

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

The invention discloses an electric vehicle power battery SOC intelligent detection device which is characterized in that the intelligent detection device includes a battery parameter acquisition platform and a battery SOC estimation system, the battery parameter acquisition platform collects real-time parameters of voltage, current and temperature of an electric vehicle power battery group, the battery SOC estimation system estimates an SOC value through the collected parameters, and a battery SOC system is a nonlinear, time-delay and multivariable coupling complex real-time system with a high requirement. According to the device, a problem that a conventional detection device can not obtain an ideal effect of the intelligent detection of the electric vehicle power battery SOC.

The invention relates to a method for rapidly representing sludge dewatering performance. The method comprises the following steps of: collecting 100ml of raw sludge or concentrated sludge; filtering with a filter membrane of 0.45mu m to obtain 10mL of sludge supernatant; measuring soluble organic carbon (DOC) of the supernatant; diluting the supernatant with distilled water until the DOC is less than 10mg / L; collecting a three-dimensional fluorescence spectrum (EEM) of the diluted supernatant; substituting the collected EEM spectrum into a database to analyze a parallel factor (PARAFAC) by using a DOMF1uor tool kit of Mat1ab 7.0 data processing software to determine a score of a component with excitation and transmission wavelength of (220,280) / 350nm; and multiplying the score by a diluting multiple of a sample and substituting the product into a sludge dewatering performance judgment relational formula to represent the sludge dewatering performance. By the method, the sludge dewatering performance can be rapidly detected without chemical reagent, and the detection can be completed only through filtering, diluting and three-dimensional fluorescence spectrum; and the method can be applied to monitoring the dewatering performance of various types of sludge of sanitary sewage treatment factories, industrial sewage biological treatment factories and the like.

The invention relates to a fluorescence nano-material, and particularly relates to a preparation method of a fluorescence probe and applications of the fluorescence probe. The fluorescence probe has characteristics of strong fluorescence signals, high stability and good water solubility. The fluorescence probe is prepared by connecting reduced carbon quantum dots capable of emitting blue fluorescence to single-stranded DNA. The reduced carbon quantum dots are prepared from carbon quantum dots, and are prepare by subjecting the carbon quantum dots to surface modification by adopting sodium borohydride as a reductant and adopting a chemical reduction method so as to obtain the reduced carbon quantum dots capable of emitting the blue fluorescence. Detection of the target DNA is achieved by utilization of fluorescence quenching-recovery processes of the fluorescence probe.

The invention discloses an electric automobile power battery SOC (State of Charge) detection system. The characteristics lie in that the detection system comprises a battery parameter acquisition platform and a battery SOC estimation system, the battery parameter acquisition platform is responsible for real-time parameter acquisition for the voltage, current and temperature of an automobile power battery pack, and the battery SOC estimation system can accurately estimate a battery SOC value through the acquired parameters; and the battery SOC is a non-linear, delayed, multivariable coupling and complex highly demanding real-time system. The detection system effectively solves a problem that the traditional automobile battery SOC estimation method is difficult to achieve an ideal effect.

The invention discloses an infrared spectroscopy-based rapid device special for soil nitrate nitrogen and a using method thereof. The device comprises a soil sample cell module, an excitation light source module, a detector module, a modeling system module, a result display window module and operating button modules, wherein the soil sample cell module is used for storing soil pasty samples; the excitation light source module is used for transmitting an intermediate infrared laser light source signal; an ATR (Attenuated Total Reflection) crystal module is in a diamond interface; the modeling system module is used for receiving and processing spectroscopic data information and carrying out modeling, optimizing and quantitative calculation on the content of nitrate nitrogen; the result display window module is used for carrying out digital display on the content of the nitrate nitrogen; the button modules I, II, III and IV are respectively used for sampling an ATR spectrum, saving a model algorithm, calculating an optimal model algorithm and displaying results. The device disclosed by the invention is simple in operation and less in sample demands, samples are not required to be subjected to fussy pretreatment, the detection speed is fast, no chemical reagents are used, the whole process just requires several minutes, and the device is suitable for fast sampling detection in a field and a scene.

The invention discloses an analysis method for detecting pyrazine compounds in beer. A method for combining headspace solid-phase microextraction with gas-phase chromatography / mass spectrometry analysis is adopted. The analysis method comprises the following steps of: preparation, headspace solid-phase microextraction, gas-phase chromatography / mass spectrometry analysis, and detection of components to be detected which enter a detector, wherein in the solid-phase microextraction procedure, a 75mu m polydimethylsiloxane extraction fiber head is adopted, and the extraction effect is favorable. By the method, the analysis method for detecting volatile pyrazine components in the beer is established, namely a headspace solid-phase microextraction method is combined with a gas-phase chromatography / mass spectrometry. The method has the characteristics of low sample requirement, high efficiency and the like, and is quick, simple, accurate and sensitive.

The invention discloses an intelligent SOC (State of Charge) prediction device for an electric vehicle power battery, which is characterized by comprising a battery parameter acquisition platform and a battery SOC prediction system, wherein the battery parameter acquisition platform is used to acquire real-time parameters of voltage, current, temperature, and ambient temperature of the vehicle power battery pack; and the battery SOC prediction system is used to predict the battery SOC value through the acquired real-time parameters. The battery SOC is a nonlinear, delayed, multivariable-coupling, and complex real-time system with extremely high real-time performance requirements. The problem that the conventional prediction device can not achieve ideal battery SOC prediction precision effects can be effectively solved.

The invention provides a device for measuring the respiration of microbes. The device is equipped with a tubular reaction cavity, wherein, the inner wall of the tubular reaction cavity is equipped with a reaction sieve which divides the reaction cavity into an upper part and a lower part; the orifice of the tubular reaction cavity is equipped with a seal plug; the seal plug is equipped with a through hole; an electrode tip hermetically passes through the through hole to extend into the upper part of the tubular reaction cavity; the tubular reaction cavity is vertically placed in a thermostatic water bath, and the thermostatic water bath is equipped with a water inlet and a water outlet which are communicated with a thermostatic water tank for circulation; the bottom surface of the tubularreaction cavity is contacted with the bottom surface of the thermostatic water bath; the top of the thermostatic water bath is equipped with a hole which is hermetically matched with the external diameter of the orifice of the tubular reaction cavity; a magnetic sheet is placed on the bottom surface of the tubular reaction cavity; the bottom surface of the thermostatic water bath is placed on a magnetic stirrer; the tubular reaction cavity is filled with a reaction solution; and a biological sample is placed on the reaction sieve, and the sieve pore is smaller than the volume of the biological sample.

The invention relates to a fluorescence labeling tracer and a preparation method thereof. The fluorescence labeling tracer is a product which is obtained by reacting a polymer of acrylic acid-hydroxy-propyl acrylate copolymer or acrylic acid-acrylamide copolymer and an organic fluorescence derivating agent of L-tryptophan, tyrosine, sulfanilic acid or sodium sulfanilate. The fluorescence labeling tracer is high in sensitivity and selectivity. When used for detecting the concentration of a water treatment agent in an industrial water system, the fluorescence labeling tracer is quick and convenient, and high in repeatability, samples can be taken easily, the required amount of samples is small, and the fluorescence labeling tracer can be used for directly and continuously detecting the running condition of a circulating water system.

The invention discloses a method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma. The method comprises steps of: extracting a blood plasma sample with a solvent, processing the sample liquid with a solid phase extraction technology, adding the sample liquid into a small processed solid-phase extraction column, washing the column with acetic acid and a methanol-acetic acid solution, eluting with ethyl acetate and 1-chorobutane, collecting the eluate, blowing the eluate to dry with nitrogen, dissolving residue with methanol so as to obtain a sample solution to be tested, and accurately measuring the content of the artesunate and the content of the dihydroartemisinin in the sheep blood plasma by utilization of HPLC-MS / MS. The lowest detectable limit of the artesunate is 0.1 ng*mL<-1>. The lowest detectable limit of the dihydroartemisinin is 1 ng*mL<-1>. The method reduces influences of impurities, enriches the sample concentration and is high in specificity and good in separation effect. Linearity, stability, reproducibility, and recovery tests of the method satisfy good technical requirements. The method lays methodology foundations for research of pharmacokinetics and bioequivalence of the medicine inside animals.

The invention belongs to a biological indicator for rapid estimation of an ionizing radiation dose, and in particular relates to an application of soluble receptor tyrosine kinase AXL (sAXL) serving as the biological indicator for the rapid estimation of the ionizing radiation dose. After radiation, the concentration of the sAXL in serum or plasma is decreased along with the increase of the radiation dose, so that a good dose-effect relationship is formed after a period of radiation and can be detected rapidly by methods which are simple and easy to implement, such as ELISA (Enzyme Linked Immunosorbent Assay) and protein chips. During the detection, the required sample quantity is small; and the sampling is carried out conveniently. Thus, the sAXL can be taken as the biological indicator for the rapid estimation of the ionizing radiation dose. In addition, the ionizing radiation dose of a human body or an animal subjected to the ionizing radiation can be estimated by utilizing the content of the sAX in serum or plasma.

The invention relates to an exosome separation and purification device and a separation and purification method thereof. The device comprises an upper micro-fluidic chip, a filter membrane and a lowermicro-fluidic chip, and the filter membrane is arranged between the upper micro-fluidic chip and the lower micro-fluidic chip. Sample liquid is injected into the upper micro-fluidic chip through oneinlet of the upper micro-fluidic chip, and after the sample liquid is filtered through the filtering membrane, an exosome is obtained from one inlet of the lower micro-fluidic chip. According to the method disclosed by the invention, impurity protein in the exosome can be removed, the whole exosome detection and analysis time of the technology is less than 1 hour, and the technology is stable. A sample obtained by the device can be directly subjected to mass spectrometry, and the main signals of a graph obtained are consistent with those of a mass spectrum detection graph obtained after exosome cyst separation and purification through an existing standard ultracentrifugation method. The method has the advantages of being low in cost, small in amount of samples needed, rapid, convenient touse, accurate, stable, capable of avoiding the defects that time and resources are consumed in the ultracentrifugation technology and the like and wide in biological and medical application prospect.

The invention relates to the technical field of electrochemical detection, in particular to a bilirubin electrochemical transducer and a preparation method thereof. The bilirubin electrochemical transducer comprises an electrode, a bilirubin oxidase layer and an anti-interference enzyme layer, wherein the bilirubin oxidase layer is arranged between the electrode and the anti-interference enzyme layer, and comprises bilirubin oxidase and an oxidation-state electronic mediator; the anti-interference enzyme layer comprises ascorbic acid oxidase. The bilirubin electrochemical transducer has the advantages of being high in sensitivity, wide in linearity range, low in sample demand, high in anti-interference capacity, and short in detection time.

The invention discloses an oil content detecting method for wastewater. The oil content detecting method comprises the following steps: preparing 500mL of a 3ppm oil-water solution, 500mL of a 6ppm oil-water solution, 500mL of a 10.4ppm oil-water solution, 500mL of a 14.2ppm oil-water solution and 500mL of a 18.0ppm oil-water solution by adopting an ultraviolet working curve process; and extracting twice by totally adopting 25mL of cyclohexane, adding 10g of NaCl into oil-water solutions before extracting, regulating the pH values of the solutions to 3 with sulfuric acid, removing water for extracted cyclohexane oil solutions with special infrared anhydrous sodium sulfate, keeping the volume constant be 100mL, and detecting the concentration of constant-volume solutions through an ultraviolet spectrophotometer. The ultraviolet spectrophotometry is used for measuring content of oil according to characteristic absorption of aromatic compounds and common double-bond compounds in petroleum and products thereof in a 215-260nm ultraviolet region. The oil content detecting method for the wastewater can be used for detecting the industrial wastewater with the content of the oil of lower than 1.0*10<-7> by adopting an ultraviolet working curve process principle, and has the advantages of being low in equipment cost, high in sensitivity, good in repeatability, easy to sample, small in needed sample amount and the like.

The invention belongs to a biological index for rapidly estimating the ionizing radiation dosage and particularly relates to an application of soluble CD30 (sCD30) as a biological index for rapidly estimating the ionizing radiation dosage. After radiation, the concentration of the sCD30 in blood serum or blood plasma is reduced along with the increasing of the radiation dosage; after the radiation is carried out within a certain time range, a very good dosage-effect relation exists; the rapid detection can be carried out by using a simple and feasible method through ELISA (Enzyme Linked Immunosorbent Assay), a protection chip and the like; a little amount of sample is needed by the detection and the sampling is convenient. Therefore, the sCD30 can be used as the biological index for rapidly estimating the ionizing radiation dosage; the radiation dosage of a human body and an animal after ionizing radiation is rapidly calculated by adopting the content of the sCD30 in the blood serum or the blood plasma.

The invention discloses a determination method for the content of irisflorentin in animal serum. The high performance liquid chromatography-mass spectrometry technology is applied to determination of the content of irisflorentin in animal serum; and kaempferol is used as internal standard for determination of the content of irisflorentin in animal serum. According to the method, sample treatment is simple and easy to operate, and a recovery rate meets requirements of analysis and detection; and as the HPLC-MS / MS technology is applied to determination of the content of irisflorentin in animal serum, detection sensitivity of detected components is improved, good resolution is realized, analysis time is short, the desired amount of a sample is small, the usage amount of a solvent is small, content determination results are accurate, and repeatability and good stability are achieved.

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

The invention discloses an intelligent garbage cleaning and environmental parameter big data Internet of Things system, which is composed of an environmental parameter acquisition and control platform and an environmental parameter processing and garbage cleaning subsystem, and is characterized in that the environmental parameter acquisition and control platform is used for detecting, adjusting and monitoring environmental parameters; the environmental parameter big data processing subsystem is used for controlling environmental parameter processing and garbage cleaning; the invention effectively solves the problems that the existing environmental garbage cleaning does not influence the environmental quality according to the nonlinearity, large lag, large and complex environmental area and the like of environmental parameter change, and does not predict the environmental parameters, accurately detect the environmental parameters and adjust the garbage cleaning device; therefore, environmental parameter prediction and environmental management are greatly influenced.

The invention discloses a set of markers and methods for screening high-flavor tea tree resources suitable for making oolong tea, and the markers are composed of indole, nerolidol and Jasmine lactone.The landmark in the invention can be applied to the scoring and rapid screening of high-flavor tea tree resources suitable for making oolong tea. The method for screening high-flavor tea tree resources suitable for making oolong tea in the invention has the advantages of simple operation, short time consumption, small sample demand and high reliability, and can be applied to large-scale resourcesample screening.

The invention provides a method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry and application and relates to the technical field of chemical detection. The method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry is used for taking ketoprofen-D3 as a detection internal standard and performing quantification by using an internal standard curve method. The method is high in specificity and low in detection limit; meanwhile, relevant coefficients in the linear range of a standard curve are greater than 0.999 and show that linear relation in the linear range is good and the repeatability is high; in addition, the method also has the advantages of short analysis time and low quantity of needed samples; the sample analyzing efficiency can be greatly improved; in conclusion, the whole operation process of the method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry is simple and is easy to implement; the method is scientific, reliable and controllable, is suitable for practical application and popularization and has a broad application prospect.

The invention discloses an electric automobile power battery SOC (State of Charge) detection system. The characteristics lie in that the detection system comprises a battery parameter acquisition platform and a battery SOC estimation system, the battery parameter acquisition platform is responsible for real-time parameter acquisition for the voltage, current and temperature of an automobile power battery pack, and the battery SOC estimation system can accurately estimate a battery SOC value through the acquired parameters; and the battery SOC is a non-linear, delayed, multivariable coupling and complex highly demanding real-time system. The detection system effectively solves a problem that the traditional automobile battery SOC estimation method is difficult to achieve an ideal effect.

The invention discloses a novel microbial sensor for detecting chromium ions. The novel microbial sensor comprises a polycarbonate (PC) detection base plate, an alloy coating conduction channel is arranged on the surface of the PC detection base plate, one end of the alloy coating conduction channel is connected with a leading wire, the other end of the alloy coating conduction channel is a detection coating, a layer of PC cover plate is covered above the detection coating, a detection pool which corresponds to the detection coating is arranged on the PC cover plate, the detection coating serves as the bottom surface of the detection pool, a chromium ion induction layer is coated on the surface of the detection coating of the detection pool, the chromium ion induction layer is composed of graphite powders, ochrobactrum anthropi freeze-dried powders and liquid paraffin according to a mass ratio of 45:45:10, and the graphite powders, the ochrobactrum anthropi freeze-dried powders and the liquid paraffin are uniformly mixed and tiled on the surface of the detection coating. The novel microbial sensor for detecting the chromium ions has the advantages that the required quantity of samples is small, the detection time is short, the sensitivity is high, the lower limit of detection is low, the novel microbial sensor is extremely easy to carry about, the real-time monitoring is convenient, most importantly the manufacturing cost is low, the mass production is achieved, and the stability and the biological selectivity are high.

The invention provides a device for measuring the respiration of microbes. The device is equipped with a tubular reaction cavity, wherein, the inner wall of the tubular reaction cavity is equipped with a reaction sieve which divides the reaction cavity into an upper part and a lower part; the orifice of the tubular reaction cavity is equipped with a seal plug; the seal plug is equipped with a through hole; an electrode tip hermetically passes through the through hole to extend into the upper part of the tubular reaction cavity; the tubular reaction cavity is vertically placed in a thermostatic water bath, and the thermostatic water bath is equipped with a water inlet and a water outlet which are communicated with a thermostatic water tank for circulation; the bottom surface of the tubularreaction cavity is contacted with the bottom surface of the thermostatic water bath; the top of the thermostatic water bath is equipped with a hole which is hermetically matched with the external diameter of the orifice of the tubular reaction cavity; a magnetic sheet is placed on the bottom surface of the tubular reaction cavity; the bottom surface of the thermostatic water bath is placed on a magnetic stirrer; the tubular reaction cavity is filled with a reaction solution; and a biological sample is placed on the reaction sieve, and the sieve pore is smaller than the volume of the biological sample.

The invention discloses a strawberry greenhouse temperature intelligent detection device based on an empirical mode decomposition model, which is characterized in that: the detection device includes a strawberry greenhouse environment parameter acquisition platform based on a CAN field bus and a strawberry greenhouse temperature intelligent prediction system; the present invention It not only effectively solves many problems in the traditional strawberry greenhouse environment due to unreasonable design, backward equipment, and imperfect control system, but also effectively solves the existing strawberry greenhouse environmental monitoring system. Due to the characteristics of non-linearity, large lag, and complex temperature changes of the strawberry greenhouse environment, the temperature of the strawberry greenhouse environment is monitored and predicted, which greatly affects the regulation of the strawberry greenhouse environment temperature.

The invention relates to a high performance liquid chromatography method for jointly, rapidly and accurately detecting the contents of melanin and inosinic acid in chicken of silky fowl. The method comprises the following steps of: (1) carrying out protease oscillating enzymolysis on a chicken sample for 24 hours, centrifuging to obtain liquid supernatant which serves as inosinic acid to-be-detected solution; (2) orderly washing precipitate which is subjected to enzymolysis and centrifugation with diethyl ether, absolute ethyl alcohol and ultrapure water, removing lipid and impurities, so as to obtain a melanin crude product, oxidizing the crude product into 2, 3, 5-tricarboxylic acid pyrrole under alkaline condition, extracting with diethyl ether, and drying; and (3) dissolving the dry crystal with the to-be-detected inosinic acid solution, loading and detecting the sample on a high performance liquid chromatograph, and quantifying by an external standard method. The high performance liquid chromatography method only needs little sample to simultaneously detect the contents of the melanin and inosinic acid in the chicken sample of silky fowl, moreover, the method is simple in experimental operation steps, saves detection cost, and is beneficial for acceleration of screening excellent silky fowl breed (series) or combination with both outstanding functions and flavor substances.

Provided is an intelligent prediction system for a power battery SOC of an electric vehicle, the intelligent prediction system comprising a battery parameter collection platform and a battery SOC prediction system, wherein the battery parameter collection platform is used for collecting real-time parameters of the voltage, current and temperature of a vehicle power battery pack and an ambient temperature; and the battery SOC prediction system predicts, through the collected real-time parameters, a battery SOC value. The battery SOC is a real-time system which is non-linear, time-delayed, multi-variable coupled and complex, with high demands on real time performance. The intelligent prediction system effectively solves the problem that it is difficult for a conventional prediction device to obtain an ideal effect of battery SOC prediction accuracy.

The invention relates to the technical field of drug analysis, and specifically discloses a method for detecting related substances in rifampicin. The method comprises: preparing a test solution and a reference solution, and detecting by liquid chromatography-mass spectrometry, wherein the liquid chromatography adopts a C18 chromatographic column, and a formic acid aqueous solution with a volume concentration of 0.8‰~1.2‰ is used as a flow Phase A, using acetonitrile as mobile phase B, gradient elution; the mass spectrometer adopts ESI ion source, positive ion detection, SIM mode. The detection method provided by this application has the advantages of simple method, stability, high precision, good reproducibility, etc., and can quickly and accurately detect the impurities N-methylpiperazine and 1-amino-4- The whole operation process is reliable and controllable, suitable for practical application and promotion, and has broad application prospects.