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Novel microbial sensor for detecting chromium ions

A microbial sensor and chromium ion technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of lack of localized strains and limited types of strains, and achieve low production cost, easy portability, and small sample requirements Effect

Active Publication Date: 2013-02-27
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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Problems solved by technology

It has been mentioned in the literature that some representative bacteria that are resistant to chromium or can reduce and attenuate it include: Pseudomonas aeruginosaA2Chr, Bacillus megaterium TKW3, Enterobavter cloacae HO1, and Bacillus firmus, etc. (Ganguli and Tripathi, 2002; Cheung et al., 2006; Sau et al.,2008), this type of bacteria can be used to remove hexavalent chromium pollution, but the types of these strains are still very limited, especially the lack of some localized strains

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  • Novel microbial sensor for detecting chromium ions
  • Novel microbial sensor for detecting chromium ions
  • Novel microbial sensor for detecting chromium ions

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Embodiment Construction

[0024] The following examples are to further illustrate the present invention, rather than limit the present invention.

[0025] 1. Preparation of a novel microbial sensor for detecting chromium ions:

[0026] 1. Selection of bacterial strains: Tolerance experiment of Pallidum GIMT1.002 to chromium ions

[0027] Inoculate Pallidum GIMT1.002 in 10 -7 mol / L~10 -3 mol / L chromium nitrate LB medium, in optical density OD 600 1. Under the condition of shaking at 30°C for 48 hours, the growth curve of the human Pallidum GIMT1.002 strain was determined. like figure 1 As shown, the strain no matter at low concentration (10 -7 mol / L) of chromium nitrate solution or in high concentration (10 -3 mol / L) in the chromium nitrate solution showed a state of continuous growth, about 30 hours into the stationary phase (the solid line in the figure is the growth curve of the strain in the medium without chromium ions). Therefore, it was proved that P. hominis GIMT1.002 is tolerant to chrom...

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Abstract

The invention discloses a novel microbial sensor for detecting chromium ions. The novel microbial sensor comprises a polycarbonate (PC) detection base plate, an alloy coating conduction channel is arranged on the surface of the PC detection base plate, one end of the alloy coating conduction channel is connected with a leading wire, the other end of the alloy coating conduction channel is a detection coating, a layer of PC cover plate is covered above the detection coating, a detection pool which corresponds to the detection coating is arranged on the PC cover plate, the detection coating serves as the bottom surface of the detection pool, a chromium ion induction layer is coated on the surface of the detection coating of the detection pool, the chromium ion induction layer is composed of graphite powders, ochrobactrum anthropi freeze-dried powders and liquid paraffin according to a mass ratio of 45:45:10, and the graphite powders, the ochrobactrum anthropi freeze-dried powders and the liquid paraffin are uniformly mixed and tiled on the surface of the detection coating. The novel microbial sensor for detecting the chromium ions has the advantages that the required quantity of samples is small, the detection time is short, the sensitivity is high, the lower limit of detection is low, the novel microbial sensor is extremely easy to carry about, the real-time monitoring is convenient, most importantly the manufacturing cost is low, the mass production is achieved, and the stability and the biological selectivity are high.

Description

technical field [0001] The invention belongs to the field of microbial microelectrodes, in particular to a novel microbial sensor for detecting chromium ions. Background technique: [0002] Chromium is an element that exists widely in nature, mainly in the form of trivalent chromium and hexavalent chromium. Trivalent chromium participates in the metabolism of sugar and fat in humans and animals, and is an essential trace element for the human body; hexavalent chromium is a clear harmful element that can precipitate certain proteins in human blood, causing anemia, nephritis, neuritis, etc. Long-term exposure to hexavalent chromium can also cause respiratory inflammation and induce lung cancer or cause invasive skin damage. Severe hexavalent chromium poisoning can also cause death. Because chromium is one of the substances widely used in the industry, it is mostly used in steel manufacturing, metal corrosion and electroplating industries, etc. If the chromium-containing wast...

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 郭诗韵许玫英张国霞陈杏娟孙国萍郭俊曾国驱
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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