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Method for detecting alkaloid in tobacco

A detection method and alkaloid technology, applied in the detection field, can solve problems such as complex operation process, few types of alkaloids, poor reproducibility, etc., and achieve the effect of good reproducibility, simple steps and high recovery rate

Inactive Publication Date: 2012-04-04
SHANGHAI HAIDI GARDENING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the complex operation process existing in the detection of alkaloids in tobacco in the prior art, poor reproducibility, low recovery rate, and the technical problems of fewer types of alkaloids measured

Method used

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  • Method for detecting alkaloid in tobacco
  • Method for detecting alkaloid in tobacco
  • Method for detecting alkaloid in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] 1. Preparation of injection solution

[0025] Accurately weigh 0.5g of the sample and place it in a 100ml Erlenmeyer flask with a stopper, then add 2mol / L NaOH aqueous solution, soak and sonicate, then add 10μl of 5mg / ml quinoline as an internal standard, centrifuge at 4000rpm for 10min to take the supernatant, use CH 2 CI 2 Extract 3 times, combine the organic layers, then add a small amount of anhydrous sodium sulfate, refrigerate overnight, and use a rotary evaporator to concentrate the extract to 1ml in a water bath at 45°C to obtain a sample solution;

[0026] 2. Linear regression equation and determination of detection limit

[0027] Accurately measure 24.75μl of nicotine and dissolve it in 1ml of dichloromethane to make 25mg / ml nicotine standard solution;

[0028] Accurately weigh 25 mg of nornicotine and dissolve it in 1 ml of dichloromethane, then measure 80 μl and dissolve it in 1 ml of dichloromethane to prepare a 2 mg / ml nornicotine standard stock solutio...

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Abstract

The invention relates to a method for detecting alkaloid in tobacco. The method comprises steps that: step 1, a tobacco sample is added to an alkaline solution; the mixture is subject to an ultrasonic treatment; an organic solvent containing quinoline is added to the mixture, and the mixture is processed through oscillation extraction; an organic layer is condensed, such that a sample solution is obtained; step 2, the sample solution is detected by using a gas chromatograph-mass spectrometer, such that alkaloid in tobacco is processed through quantitative and qualitative determinations. The detection method provided by the invention is advantaged in simple steps, convenient operation, good reproducibility, high sensitivity, and high recovery rate. With the method provided by the invention, all the components of tobacco alkaloid can be completely extracted. Therefore, the method can be effectively used in analysis and detection of tobacco alkaloid.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to a method for detecting alkaloids in tobacco. Background technique [0002] Alkaloids are the main nitrogen-containing heterocyclic compounds in tobacco leaves, and there are many types, the most important of which is nicotine, which is a unique alkaloid in tobacco. The vast majority of tobacco alkaloids are 2-pyridyl derivatives, mainly in the form of organic acids and inorganic acid salts, and their content will affect the taste and quality of cigarettes. Due to the extremely low content of alkaloids, it cannot be accurately detected by conventional methods. The commonly used analysis methods mainly include gas chromatography, high performance liquid chromatography, capillary electrophoresis and supercritical liquid method. However, these methods have the problems of complex operation process, poor reproducibility, low recovery rate, and fewer types of alkaloids measured. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08
Inventor 丁国才
Owner SHANGHAI HAIDI GARDENING
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